Catechol Siderophores from a Mangrove-Derived Bacteria <i>Serratia marcescens</i> F2-2 and Their Cytotoxic Activity
<i>Serratia marcescens</i> is a common Gram-negative and facultative anaerobic bacillus that produces serratiochelins with several bioactivities. In this study, four catechol siderphores (<b>1</b>–<b>4</b>), including two new ones named serratiochelins E (<b>...
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2025-05-01
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| author | Gang Zhang Xunming Wang Xingwang Zhang Lin Ye Longyang Ke Shimin Fan Xuan Hong Guoqiang Li Bingye Yang Lianzhong Luo |
| author_facet | Gang Zhang Xunming Wang Xingwang Zhang Lin Ye Longyang Ke Shimin Fan Xuan Hong Guoqiang Li Bingye Yang Lianzhong Luo |
| author_sort | Gang Zhang |
| collection | DOAJ |
| description | <i>Serratia marcescens</i> is a common Gram-negative and facultative anaerobic bacillus that produces serratiochelins with several bioactivities. In this study, four catechol siderphores (<b>1</b>–<b>4</b>), including two new ones named serratiochelins E (<b>1</b>) and F (<b>2</b>), were obtained from the fermentation of a mangrove-derived bacterium, <i>S</i>. <i>marcescens</i> F2-2. The structures were elucidated with various spectroscopic methods such as NMR and HR-ESI-MS. Absolute and geometric configurations of the new compounds were established by employing quantum NMR calculations in conjunction with DP4+ probability analysis, ECD calculations, and the advanced Marfey’s method. The bioactivity test showed that serratiochelin B (<b>3</b>) displayed weak but selective cytotoxicity against HepG2 cancer cells with an IC<sub>50</sub> of 50.6 μmol/L and could trigger apoptosis through both Bcl-2/Bax/caspase-3 and Fas/FasL/caspase-8 signaling pathways. These findings deepen the understanding of siderophores of <i>S. marcescens</i> and provide a lead for research on anti-liver cancer drugs. |
| format | Article |
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| institution | OA Journals |
| issn | 1660-3397 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Marine Drugs |
| spelling | doaj-art-e774a2f7f72c4eb1999f8b1ad0addc3b2025-08-20T02:21:10ZengMDPI AGMarine Drugs1660-33972025-05-0123624110.3390/md23060241Catechol Siderophores from a Mangrove-Derived Bacteria <i>Serratia marcescens</i> F2-2 and Their Cytotoxic ActivityGang Zhang0Xunming Wang1Xingwang Zhang2Lin Ye3Longyang Ke4Shimin Fan5Xuan Hong6Guoqiang Li7Bingye Yang8Lianzhong Luo9Engineering Research Center of Marine Biopharmaceutical Resource of Fujian Province University, Xiamen Medical College, Xiamen 361023, ChinaEngineering Research Center of Marine Biopharmaceutical Resource of Fujian Province University, Xiamen Medical College, Xiamen 361023, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaEngineering Research Center of Marine Biopharmaceutical Resource of Fujian Province University, Xiamen Medical College, Xiamen 361023, ChinaEngineering Research Center of Marine Biopharmaceutical Resource of Fujian Province University, Xiamen Medical College, Xiamen 361023, ChinaEngineering Research Center of Marine Biopharmaceutical Resource of Fujian Province University, Xiamen Medical College, Xiamen 361023, ChinaXiamen Key Laboratory of Marine Medicinal Natural Product Resources, Xiamen Medical College, Xiamen 361023, ChinaKey Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, ChinaEngineering Research Center of Marine Biopharmaceutical Resource of Fujian Province University, Xiamen Medical College, Xiamen 361023, ChinaEngineering Research Center of Marine Biopharmaceutical Resource of Fujian Province University, Xiamen Medical College, Xiamen 361023, China<i>Serratia marcescens</i> is a common Gram-negative and facultative anaerobic bacillus that produces serratiochelins with several bioactivities. In this study, four catechol siderphores (<b>1</b>–<b>4</b>), including two new ones named serratiochelins E (<b>1</b>) and F (<b>2</b>), were obtained from the fermentation of a mangrove-derived bacterium, <i>S</i>. <i>marcescens</i> F2-2. The structures were elucidated with various spectroscopic methods such as NMR and HR-ESI-MS. Absolute and geometric configurations of the new compounds were established by employing quantum NMR calculations in conjunction with DP4+ probability analysis, ECD calculations, and the advanced Marfey’s method. The bioactivity test showed that serratiochelin B (<b>3</b>) displayed weak but selective cytotoxicity against HepG2 cancer cells with an IC<sub>50</sub> of 50.6 μmol/L and could trigger apoptosis through both Bcl-2/Bax/caspase-3 and Fas/FasL/caspase-8 signaling pathways. These findings deepen the understanding of siderophores of <i>S. marcescens</i> and provide a lead for research on anti-liver cancer drugs.https://www.mdpi.com/1660-3397/23/6/241<i>S. marcescens</i>serratiochelinscytotoxicityapoptosis |
| spellingShingle | Gang Zhang Xunming Wang Xingwang Zhang Lin Ye Longyang Ke Shimin Fan Xuan Hong Guoqiang Li Bingye Yang Lianzhong Luo Catechol Siderophores from a Mangrove-Derived Bacteria <i>Serratia marcescens</i> F2-2 and Their Cytotoxic Activity Marine Drugs <i>S. marcescens</i> serratiochelins cytotoxicity apoptosis |
| title | Catechol Siderophores from a Mangrove-Derived Bacteria <i>Serratia marcescens</i> F2-2 and Their Cytotoxic Activity |
| title_full | Catechol Siderophores from a Mangrove-Derived Bacteria <i>Serratia marcescens</i> F2-2 and Their Cytotoxic Activity |
| title_fullStr | Catechol Siderophores from a Mangrove-Derived Bacteria <i>Serratia marcescens</i> F2-2 and Their Cytotoxic Activity |
| title_full_unstemmed | Catechol Siderophores from a Mangrove-Derived Bacteria <i>Serratia marcescens</i> F2-2 and Their Cytotoxic Activity |
| title_short | Catechol Siderophores from a Mangrove-Derived Bacteria <i>Serratia marcescens</i> F2-2 and Their Cytotoxic Activity |
| title_sort | catechol siderophores from a mangrove derived bacteria i serratia marcescens i f2 2 and their cytotoxic activity |
| topic | <i>S. marcescens</i> serratiochelins cytotoxicity apoptosis |
| url | https://www.mdpi.com/1660-3397/23/6/241 |
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