Catechol Siderophores from a Mangrove-Derived Bacteria <i>Serratia marcescens</i> F2-2 and Their Cytotoxic Activity

<i>Serratia marcescens</i> is a common Gram-negative and facultative anaerobic bacillus that produces serratiochelins with several bioactivities. In this study, four catechol siderphores (<b>1</b>–<b>4</b>), including two new ones named serratiochelins E (<b>...

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Main Authors: Gang Zhang, Xunming Wang, Xingwang Zhang, Lin Ye, Longyang Ke, Shimin Fan, Xuan Hong, Guoqiang Li, Bingye Yang, Lianzhong Luo
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Marine Drugs
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Online Access:https://www.mdpi.com/1660-3397/23/6/241
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Summary:<i>Serratia marcescens</i> is a common Gram-negative and facultative anaerobic bacillus that produces serratiochelins with several bioactivities. In this study, four catechol siderphores (<b>1</b>–<b>4</b>), including two new ones named serratiochelins E (<b>1</b>) and F (<b>2</b>), were obtained from the fermentation of a mangrove-derived bacterium, <i>S</i>. <i>marcescens</i> F2-2. The structures were elucidated with various spectroscopic methods such as NMR and HR-ESI-MS. Absolute and geometric configurations of the new compounds were established by employing quantum NMR calculations in conjunction with DP4+ probability analysis, ECD calculations, and the advanced Marfey’s method. The bioactivity test showed that serratiochelin B (<b>3</b>) displayed weak but selective cytotoxicity against HepG2 cancer cells with an IC<sub>50</sub> of 50.6 μmol/L and could trigger apoptosis through both Bcl-2/Bax/caspase-3 and Fas/FasL/caspase-8 signaling pathways. These findings deepen the understanding of siderophores of <i>S. marcescens</i> and provide a lead for research on anti-liver cancer drugs.
ISSN:1660-3397