Inhibition of IGF-1Rα affects the differentiation fate of rat optic cup-derived retinal stem cells to retinal ganglion cells in vitro
AIM: To explore the impact of insulin-like growth factor-1 receptor α (IGF-1Rα) on the differentiation fate of optic-cup-derived retinal stem cells (OC-RSCs) into retinal ganglion cells (RGCs) in vitro. METHODS: OC-RSCs were isolated from optic cups of rats on embryonic day 12.5, and high-purity OC-...
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Press of International Journal of Ophthalmology (IJO PRESS)
2025-04-01
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| Series: | International Journal of Ophthalmology |
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| Online Access: | http://ies.ijo.cn/en_publish/2025/4/20250403.pdf |
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| author | Qi-You Li Xiao-Ling Tan Hai-Wei Xu Yu-Xiao Zeng Xiao-Yong Huang |
| author_facet | Qi-You Li Xiao-Ling Tan Hai-Wei Xu Yu-Xiao Zeng Xiao-Yong Huang |
| author_sort | Qi-You Li |
| collection | DOAJ |
| description | AIM: To explore the impact of insulin-like growth factor-1 receptor α (IGF-1Rα) on the differentiation fate of optic-cup-derived retinal stem cells (OC-RSCs) into retinal ganglion cells (RGCs) in vitro. METHODS: OC-RSCs were isolated from optic cups of rats on embryonic day 12.5, and high-purity OC-RSCs were obtained by conditioned culture and passage. Differentiation of OC-RSCs into RGCs under different serum concentrations was examined using flow cytometry, and the serum concentration with high interference with differentiation ratio was selected. Furthermore, the effect of blocking IGF-1Rα on the differentiation of OC-RSCs into RGCs was analyzed through immunocytochemistry and Western blotting. RESULTS: Immunohistochemical analysis revealed IGF-1Rα was highly expressed in rat embryos at day 12.5. OC-RSCs were isolated and purified, and high-purity OC-RSCs were obtained. When 2.5% serum was administered, the ratio of differentiated RGCs (Thy-1.1 positive) decreased significantly, and the results of immunoblotting also confirmed the blockade of IGF-1Rα reduced Thy-1.1 protein expression. CONCLUSION: IGF-1Rα blocking can reduce the differentiation of OC-RSCs into RGCs. |
| format | Article |
| id | doaj-art-e7722e54768544b79c06bd8d843d294c |
| institution | Kabale University |
| issn | 2222-3959 2227-4898 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj-art-e7722e54768544b79c06bd8d843d294c2025-08-20T03:42:52ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982025-04-0118458258910.18240/ijo.2025.04.0320250403Inhibition of IGF-1Rα affects the differentiation fate of rat optic cup-derived retinal stem cells to retinal ganglion cells in vitroQi-You Li0Xiao-Ling Tan1Hai-Wei Xu2Yu-Xiao Zeng3Xiao-Yong Huang4Xiao-Yong Huang. Southwest Hospital/Southwest Eye Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China. 1176120922@qq.comDepartment of Frigid Zone Medicine, College of High Altitude Military Medicine, Third Military Medical University (Army Medical University), Chongqing 400038, ChinaSouthwest Hospital/Southwest Eye Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China; Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Chongqing 400038, ChinaSouthwest Hospital/Southwest Eye Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China; Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Chongqing 400038, ChinaSouthwest Hospital/Southwest Eye Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China; Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Chongqing 400038, ChinaAIM: To explore the impact of insulin-like growth factor-1 receptor α (IGF-1Rα) on the differentiation fate of optic-cup-derived retinal stem cells (OC-RSCs) into retinal ganglion cells (RGCs) in vitro. METHODS: OC-RSCs were isolated from optic cups of rats on embryonic day 12.5, and high-purity OC-RSCs were obtained by conditioned culture and passage. Differentiation of OC-RSCs into RGCs under different serum concentrations was examined using flow cytometry, and the serum concentration with high interference with differentiation ratio was selected. Furthermore, the effect of blocking IGF-1Rα on the differentiation of OC-RSCs into RGCs was analyzed through immunocytochemistry and Western blotting. RESULTS: Immunohistochemical analysis revealed IGF-1Rα was highly expressed in rat embryos at day 12.5. OC-RSCs were isolated and purified, and high-purity OC-RSCs were obtained. When 2.5% serum was administered, the ratio of differentiated RGCs (Thy-1.1 positive) decreased significantly, and the results of immunoblotting also confirmed the blockade of IGF-1Rα reduced Thy-1.1 protein expression. CONCLUSION: IGF-1Rα blocking can reduce the differentiation of OC-RSCs into RGCs.http://ies.ijo.cn/en_publish/2025/4/20250403.pdfinsulin-like growth factor-1 receptor αretinal ganglion cellrat optic cupretinal stem cellsdifferentiation fate |
| spellingShingle | Qi-You Li Xiao-Ling Tan Hai-Wei Xu Yu-Xiao Zeng Xiao-Yong Huang Inhibition of IGF-1Rα affects the differentiation fate of rat optic cup-derived retinal stem cells to retinal ganglion cells in vitro International Journal of Ophthalmology insulin-like growth factor-1 receptor α retinal ganglion cell rat optic cup retinal stem cells differentiation fate |
| title | Inhibition of IGF-1Rα affects the differentiation fate of rat optic cup-derived retinal stem cells to retinal ganglion cells in vitro |
| title_full | Inhibition of IGF-1Rα affects the differentiation fate of rat optic cup-derived retinal stem cells to retinal ganglion cells in vitro |
| title_fullStr | Inhibition of IGF-1Rα affects the differentiation fate of rat optic cup-derived retinal stem cells to retinal ganglion cells in vitro |
| title_full_unstemmed | Inhibition of IGF-1Rα affects the differentiation fate of rat optic cup-derived retinal stem cells to retinal ganglion cells in vitro |
| title_short | Inhibition of IGF-1Rα affects the differentiation fate of rat optic cup-derived retinal stem cells to retinal ganglion cells in vitro |
| title_sort | inhibition of igf 1rα affects the differentiation fate of rat optic cup derived retinal stem cells to retinal ganglion cells in vitro |
| topic | insulin-like growth factor-1 receptor α retinal ganglion cell rat optic cup retinal stem cells differentiation fate |
| url | http://ies.ijo.cn/en_publish/2025/4/20250403.pdf |
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