Molecular and pathological detection of jaagsiekte sheep retrovirus in lung tissues of sheep
Ovine pulmonary adenocarcinoma (OPA) is a disease of sheep that is caused by a Betaretrovirus named exogenous Jaagsiekte sheep retrovirus (exJSRV). This virus causes oncogenic transformation in lungs, and symptoms develop related to the growing tumors. Disease develops slowly with a long incubation...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kafkas University, Faculty of Veterinary Medicine
2024-11-01
|
| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=3149 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850108130297380864 |
|---|---|
| author | Nuvit COSKUN Volkan YILMAZ Emin KARAKURT Enver BEYTUT Hilmi NUHOGLU Mehmet Ozkan TIMURKAN |
| author_facet | Nuvit COSKUN Volkan YILMAZ Emin KARAKURT Enver BEYTUT Hilmi NUHOGLU Mehmet Ozkan TIMURKAN |
| author_sort | Nuvit COSKUN |
| collection | DOAJ |
| description | Ovine pulmonary adenocarcinoma (OPA) is a disease of sheep that is caused by a
Betaretrovirus named exogenous Jaagsiekte sheep retrovirus (exJSRV). This virus causes
oncogenic transformation in lungs, and symptoms develop related to the growing
tumors. Disease develops slowly with a long incubation time ranging 2-4 years. Currently
there is no serological test to evaluate the presence in the flock and also disease can
mostly be diagnosed post mortem. The aim of this study is to determine characterization
and the molecular presence exJSRV types in circulation. In this study lung tissues of
25 suspected cases were investigated. Initial diagnosis is made by histopathological
(HP) and immunohistochemical (IHC) methods. Hematoxylin & Eosin (H&E) staining
was used for examining histopathological changes. Anti JSRV capsid antibody was
used with Streptavidin-Biotin peroxidase method. Slides were examined under light
microscope and photographs were taken. All 25 cases were diagnosed as OPA with
these methods. Lung tissues embedded in paraffin were used as material for nucleic acid
extraction. Envelope gene of JSRV nucleic acid was chosen for investigating with reverse
transcription polymerase chain reaction (RT-PCR). Since paraffinized tissue blocks
were used, sensitivity was not high and only 10/25 tissues were deemed positive. Positive
amplicons were sent to sequencing. A phylogenetic tree was constructed after analyzing
the sequences. Also predicted amino acid sequences were analyzed. In conclusion we
found both type 1 and type 2 exJSRV have been circulating in the region and changes in
amino acids were detected which could lead to possible differentiation in pathogenesis. |
| format | Article |
| id | doaj-art-e76de02b83e24fdbbd0d9056fcb9e925 |
| institution | OA Journals |
| issn | 1309-2251 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj-art-e76de02b83e24fdbbd0d9056fcb9e9252025-08-20T02:38:26ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512024-11-0130680981410.9775/kvfd.2024.327023149Molecular and pathological detection of jaagsiekte sheep retrovirus in lung tissues of sheepNuvit COSKUN0Volkan YILMAZ1Emin KARAKURT2Enver BEYTUT3Hilmi NUHOGLU4Mehmet Ozkan TIMURKAN5Kafkas University, Faculty of Veterinary Medicine, Department of Virology, TR-36300 Kars-TÜRKİYEKafkas University, Faculty of Veterinary Medicine, Department of Virology, TR-36300 Kars-TÜRKİYEKafkas University Faculty of Veterinary Medicine, Department of Pathology, TR-36300 Kars-TÜRKİYEKafkas University Faculty of Veterinary Medicine, Department of Pathology, TR-36300 Kars-TÜRKİYEKafkas University Faculty of Veterinary Medicine, Department of Pathology, TR-36300 Kars-TÜRKİYEAtatürk University, Faculty of Veterinary Medicine, Department of Virology, TR-25200 Erzurum-TÜRKİYEOvine pulmonary adenocarcinoma (OPA) is a disease of sheep that is caused by a Betaretrovirus named exogenous Jaagsiekte sheep retrovirus (exJSRV). This virus causes oncogenic transformation in lungs, and symptoms develop related to the growing tumors. Disease develops slowly with a long incubation time ranging 2-4 years. Currently there is no serological test to evaluate the presence in the flock and also disease can mostly be diagnosed post mortem. The aim of this study is to determine characterization and the molecular presence exJSRV types in circulation. In this study lung tissues of 25 suspected cases were investigated. Initial diagnosis is made by histopathological (HP) and immunohistochemical (IHC) methods. Hematoxylin & Eosin (H&E) staining was used for examining histopathological changes. Anti JSRV capsid antibody was used with Streptavidin-Biotin peroxidase method. Slides were examined under light microscope and photographs were taken. All 25 cases were diagnosed as OPA with these methods. Lung tissues embedded in paraffin were used as material for nucleic acid extraction. Envelope gene of JSRV nucleic acid was chosen for investigating with reverse transcription polymerase chain reaction (RT-PCR). Since paraffinized tissue blocks were used, sensitivity was not high and only 10/25 tissues were deemed positive. Positive amplicons were sent to sequencing. A phylogenetic tree was constructed after analyzing the sequences. Also predicted amino acid sequences were analyzed. In conclusion we found both type 1 and type 2 exJSRV have been circulating in the region and changes in amino acids were detected which could lead to possible differentiation in pathogenesis.https://vetdergikafkas.org/pdf.php?id=3149histopathologyimmunohistochemistryjaagsiekte sheep retrovirusphylogenetic analysispolymerase chain reactionsheep |
| spellingShingle | Nuvit COSKUN Volkan YILMAZ Emin KARAKURT Enver BEYTUT Hilmi NUHOGLU Mehmet Ozkan TIMURKAN Molecular and pathological detection of jaagsiekte sheep retrovirus in lung tissues of sheep Kafkas Universitesi Veteriner Fakültesi Dergisi histopathology immunohistochemistry jaagsiekte sheep retrovirus phylogenetic analysis polymerase chain reaction sheep |
| title | Molecular and pathological detection of jaagsiekte sheep retrovirus in lung tissues of sheep |
| title_full | Molecular and pathological detection of jaagsiekte sheep retrovirus in lung tissues of sheep |
| title_fullStr | Molecular and pathological detection of jaagsiekte sheep retrovirus in lung tissues of sheep |
| title_full_unstemmed | Molecular and pathological detection of jaagsiekte sheep retrovirus in lung tissues of sheep |
| title_short | Molecular and pathological detection of jaagsiekte sheep retrovirus in lung tissues of sheep |
| title_sort | molecular and pathological detection of jaagsiekte sheep retrovirus in lung tissues of sheep |
| topic | histopathology immunohistochemistry jaagsiekte sheep retrovirus phylogenetic analysis polymerase chain reaction sheep |
| url | https://vetdergikafkas.org/pdf.php?id=3149 |
| work_keys_str_mv | AT nuvitcoskun molecularandpathologicaldetectionofjaagsiektesheepretrovirusinlungtissuesofsheep AT volkanyilmaz molecularandpathologicaldetectionofjaagsiektesheepretrovirusinlungtissuesofsheep AT eminkarakurt molecularandpathologicaldetectionofjaagsiektesheepretrovirusinlungtissuesofsheep AT enverbeytut molecularandpathologicaldetectionofjaagsiektesheepretrovirusinlungtissuesofsheep AT hilminuhoglu molecularandpathologicaldetectionofjaagsiektesheepretrovirusinlungtissuesofsheep AT mehmetozkantimurkan molecularandpathologicaldetectionofjaagsiektesheepretrovirusinlungtissuesofsheep |