Molecular and pathological detection of jaagsiekte sheep retrovirus in lung tissues of sheep
Ovine pulmonary adenocarcinoma (OPA) is a disease of sheep that is caused by a Betaretrovirus named exogenous Jaagsiekte sheep retrovirus (exJSRV). This virus causes oncogenic transformation in lungs, and symptoms develop related to the growing tumors. Disease develops slowly with a long incubation...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Kafkas University, Faculty of Veterinary Medicine
2024-11-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=3149 |
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| Summary: | Ovine pulmonary adenocarcinoma (OPA) is a disease of sheep that is caused by a
Betaretrovirus named exogenous Jaagsiekte sheep retrovirus (exJSRV). This virus causes
oncogenic transformation in lungs, and symptoms develop related to the growing
tumors. Disease develops slowly with a long incubation time ranging 2-4 years. Currently
there is no serological test to evaluate the presence in the flock and also disease can
mostly be diagnosed post mortem. The aim of this study is to determine characterization
and the molecular presence exJSRV types in circulation. In this study lung tissues of
25 suspected cases were investigated. Initial diagnosis is made by histopathological
(HP) and immunohistochemical (IHC) methods. Hematoxylin & Eosin (H&E) staining
was used for examining histopathological changes. Anti JSRV capsid antibody was
used with Streptavidin-Biotin peroxidase method. Slides were examined under light
microscope and photographs were taken. All 25 cases were diagnosed as OPA with
these methods. Lung tissues embedded in paraffin were used as material for nucleic acid
extraction. Envelope gene of JSRV nucleic acid was chosen for investigating with reverse
transcription polymerase chain reaction (RT-PCR). Since paraffinized tissue blocks
were used, sensitivity was not high and only 10/25 tissues were deemed positive. Positive
amplicons were sent to sequencing. A phylogenetic tree was constructed after analyzing
the sequences. Also predicted amino acid sequences were analyzed. In conclusion we
found both type 1 and type 2 exJSRV have been circulating in the region and changes in
amino acids were detected which could lead to possible differentiation in pathogenesis. |
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| ISSN: | 1309-2251 |