Associations of first-trimester TMAO and its precursors with gestational diabetes mellitus: a pilot prospective cohort study

AimsWe aimed to provide a comprehensive understanding of the associations between Trimethylamine-N-oxide (TMAO), its precursor and gestational diabetes mellitus (GDM).MethodsIn this prospective study, 940 women were included in a Chinese single -center pregnant cohort. First trimester plasma concent...

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Main Authors: Geng-Dong Chen, Ting-Ting Pang, Peng-Sheng Li, Shao-Xin Ye, Xiao-Yan Gou, Hai-Yan Wang, Dong-Xin Lin, Da-Zhi Fan, Lu-Sha Deng, Li-Juan Wang, Zi-Xing Zhou
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Nutrition
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Online Access:https://www.frontiersin.org/articles/10.3389/fnut.2025.1587863/full
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author Geng-Dong Chen
Geng-Dong Chen
Geng-Dong Chen
Ting-Ting Pang
Peng-Sheng Li
Peng-Sheng Li
Peng-Sheng Li
Shao-Xin Ye
Xiao-Yan Gou
Hai-Yan Wang
Dong-Xin Lin
Dong-Xin Lin
Dong-Xin Lin
Da-Zhi Fan
Da-Zhi Fan
Da-Zhi Fan
Lu-Sha Deng
Li-Juan Wang
Zi-Xing Zhou
Zi-Xing Zhou
Zi-Xing Zhou
author_facet Geng-Dong Chen
Geng-Dong Chen
Geng-Dong Chen
Ting-Ting Pang
Peng-Sheng Li
Peng-Sheng Li
Peng-Sheng Li
Shao-Xin Ye
Xiao-Yan Gou
Hai-Yan Wang
Dong-Xin Lin
Dong-Xin Lin
Dong-Xin Lin
Da-Zhi Fan
Da-Zhi Fan
Da-Zhi Fan
Lu-Sha Deng
Li-Juan Wang
Zi-Xing Zhou
Zi-Xing Zhou
Zi-Xing Zhou
author_sort Geng-Dong Chen
collection DOAJ
description AimsWe aimed to provide a comprehensive understanding of the associations between Trimethylamine-N-oxide (TMAO), its precursor and gestational diabetes mellitus (GDM).MethodsIn this prospective study, 940 women were included in a Chinese single -center pregnant cohort. First trimester plasma concentrations of TMAO and its precursors (betaine, choline, L-carnitine, and trimethylamine) were measured using UPLC-ESI-MS/MS. GDM and specific abnormal glucose levels (fasting glucose; one-hour post-load glucose, 1-h PG; two-hour post load glucose, 2-h PG; and 1-h PG ≥ 8.6 mmol/L) were assessed through oral glucose tolerance tests. First-trimester plasma concentrations of TMAO and its precursors were divided into quartile groups (bottom, Q1; middle, Q2 and Q3; top, Q4).ResultsAmong the subjects, 167 (17.8%) were found to have GDM. After adjusting for potential covariates, the lower groups (Q1) of L-carnitine were associated with a higher risk of GDM compared to the reference group (middle quartiles). The OR (95% CI, p) was1.56 (1.04, 2.35, p = 0.032) for L-carnitine. Specifically, the associations were mainly derived from L-carnitine and abnormal 1-h PG. The ORs (95% CI, p) were 2.00 (1.24, 3.24, p = 0.005).ConclusionLow plasma levels (bottom vs. middle quartiles) of L-carnitine the first-trimester pregnancy were associated with a higher risk of GDM and abnormal 1-h PG in Chinese pregnant women.
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spelling doaj-art-e766d485b16f4a42943dcd15cea7be962025-08-20T02:07:41ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2025-06-011210.3389/fnut.2025.15878631587863Associations of first-trimester TMAO and its precursors with gestational diabetes mellitus: a pilot prospective cohort studyGeng-Dong Chen0Geng-Dong Chen1Geng-Dong Chen2Ting-Ting Pang3Peng-Sheng Li4Peng-Sheng Li5Peng-Sheng Li6Shao-Xin Ye7Xiao-Yan Gou8Hai-Yan Wang9Dong-Xin Lin10Dong-Xin Lin11Dong-Xin Lin12Da-Zhi Fan13Da-Zhi Fan14Da-Zhi Fan15Lu-Sha Deng16Li-Juan Wang17Zi-Xing Zhou18Zi-Xing Zhou19Zi-Xing Zhou20Foshan Institute of Fetal Medicine, Foshan Women and Children Hospital Affiliated to Guangdong Medical University, Foshan, Guangdong, ChinaFoshan Key Laboratory of Stem Cell and Maternal-Child Health, Foshan Women and Children Hospital, Foshan, ChinaStem Cell Clinical Research Center, Foshan Women and Children Hospital, Foshan, ChinaDepartment of Medical Records, Foshan Women and Children Hospital Affiliated to Guangdong Medical University, Foshan, Guangdong, ChinaFoshan Institute of Fetal Medicine, Foshan Women and Children Hospital Affiliated to Guangdong Medical University, Foshan, Guangdong, ChinaFoshan Key Laboratory of Stem Cell and Maternal-Child Health, Foshan Women and Children Hospital, Foshan, ChinaStem Cell Clinical Research Center, Foshan Women and Children Hospital, Foshan, ChinaFoshan Institute of Fetal Medicine, Foshan Women and Children Hospital Affiliated to Guangdong Medical University, Foshan, Guangdong, ChinaFoshan Institute of Fetal Medicine, Foshan Women and Children Hospital Affiliated to Guangdong Medical University, Foshan, Guangdong, ChinaFoshan Institute of Fetal Medicine, Foshan Women and Children Hospital Affiliated to Guangdong Medical University, Foshan, Guangdong, ChinaFoshan Institute of Fetal Medicine, Foshan Women and Children Hospital Affiliated to Guangdong Medical University, Foshan, Guangdong, ChinaFoshan Key Laboratory of Stem Cell and Maternal-Child Health, Foshan Women and Children Hospital, Foshan, ChinaStem Cell Clinical Research Center, Foshan Women and Children Hospital, Foshan, ChinaFoshan Institute of Fetal Medicine, Foshan Women and Children Hospital Affiliated to Guangdong Medical University, Foshan, Guangdong, ChinaFoshan Key Laboratory of Stem Cell and Maternal-Child Health, Foshan Women and Children Hospital, Foshan, ChinaStem Cell Clinical Research Center, Foshan Women and Children Hospital, Foshan, ChinaDepartment of Medical Records, Foshan Women and Children Hospital Affiliated to Guangdong Medical University, Foshan, Guangdong, ChinaDepartment of Obstetrics, Foshan Women and Children Hospital Affiliated to Guangdong Medical University, Foshan, Guangdong, ChinaFoshan Institute of Fetal Medicine, Foshan Women and Children Hospital Affiliated to Guangdong Medical University, Foshan, Guangdong, ChinaFoshan Key Laboratory of Stem Cell and Maternal-Child Health, Foshan Women and Children Hospital, Foshan, ChinaStem Cell Clinical Research Center, Foshan Women and Children Hospital, Foshan, ChinaAimsWe aimed to provide a comprehensive understanding of the associations between Trimethylamine-N-oxide (TMAO), its precursor and gestational diabetes mellitus (GDM).MethodsIn this prospective study, 940 women were included in a Chinese single -center pregnant cohort. First trimester plasma concentrations of TMAO and its precursors (betaine, choline, L-carnitine, and trimethylamine) were measured using UPLC-ESI-MS/MS. GDM and specific abnormal glucose levels (fasting glucose; one-hour post-load glucose, 1-h PG; two-hour post load glucose, 2-h PG; and 1-h PG ≥ 8.6 mmol/L) were assessed through oral glucose tolerance tests. First-trimester plasma concentrations of TMAO and its precursors were divided into quartile groups (bottom, Q1; middle, Q2 and Q3; top, Q4).ResultsAmong the subjects, 167 (17.8%) were found to have GDM. After adjusting for potential covariates, the lower groups (Q1) of L-carnitine were associated with a higher risk of GDM compared to the reference group (middle quartiles). The OR (95% CI, p) was1.56 (1.04, 2.35, p = 0.032) for L-carnitine. Specifically, the associations were mainly derived from L-carnitine and abnormal 1-h PG. The ORs (95% CI, p) were 2.00 (1.24, 3.24, p = 0.005).ConclusionLow plasma levels (bottom vs. middle quartiles) of L-carnitine the first-trimester pregnancy were associated with a higher risk of GDM and abnormal 1-h PG in Chinese pregnant women.https://www.frontiersin.org/articles/10.3389/fnut.2025.1587863/fulltrimethylamine N-oxide (TMAO)TMAO precursorsL-carnitinegestational diabetes mellituspregnant women
spellingShingle Geng-Dong Chen
Geng-Dong Chen
Geng-Dong Chen
Ting-Ting Pang
Peng-Sheng Li
Peng-Sheng Li
Peng-Sheng Li
Shao-Xin Ye
Xiao-Yan Gou
Hai-Yan Wang
Dong-Xin Lin
Dong-Xin Lin
Dong-Xin Lin
Da-Zhi Fan
Da-Zhi Fan
Da-Zhi Fan
Lu-Sha Deng
Li-Juan Wang
Zi-Xing Zhou
Zi-Xing Zhou
Zi-Xing Zhou
Associations of first-trimester TMAO and its precursors with gestational diabetes mellitus: a pilot prospective cohort study
Frontiers in Nutrition
trimethylamine N-oxide (TMAO)
TMAO precursors
L-carnitine
gestational diabetes mellitus
pregnant women
title Associations of first-trimester TMAO and its precursors with gestational diabetes mellitus: a pilot prospective cohort study
title_full Associations of first-trimester TMAO and its precursors with gestational diabetes mellitus: a pilot prospective cohort study
title_fullStr Associations of first-trimester TMAO and its precursors with gestational diabetes mellitus: a pilot prospective cohort study
title_full_unstemmed Associations of first-trimester TMAO and its precursors with gestational diabetes mellitus: a pilot prospective cohort study
title_short Associations of first-trimester TMAO and its precursors with gestational diabetes mellitus: a pilot prospective cohort study
title_sort associations of first trimester tmao and its precursors with gestational diabetes mellitus a pilot prospective cohort study
topic trimethylamine N-oxide (TMAO)
TMAO precursors
L-carnitine
gestational diabetes mellitus
pregnant women
url https://www.frontiersin.org/articles/10.3389/fnut.2025.1587863/full
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