Study of Variation of <i>ACOX1</i> Gene Among Different Horse Breeds Maintained in Iran
The <i>ACOX1</i> gene is vital for fatty acid metabolism and is linked to environmental stress and physical exertion adaptation. The p.Asp237Ser variant (rs782885985) in <i>ACOX1</i> is associated with increased enzyme activity and reactive oxygen species (ROS) levels. This s...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/14/24/3566 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850240437061681152 |
|---|---|
| author | Shayan Boozarjomehri Amnieh Ali Hassanpour Sina Moghaddam Fatemeh Sakhaee Katarzyna Ropka-Molik |
| author_facet | Shayan Boozarjomehri Amnieh Ali Hassanpour Sina Moghaddam Fatemeh Sakhaee Katarzyna Ropka-Molik |
| author_sort | Shayan Boozarjomehri Amnieh |
| collection | DOAJ |
| description | The <i>ACOX1</i> gene is vital for fatty acid metabolism and is linked to environmental stress and physical exertion adaptation. The p.Asp237Ser variant (rs782885985) in <i>ACOX1</i> is associated with increased enzyme activity and reactive oxygen species (ROS) levels. This study examined the <i>ACOX1</i> polymorphism across six horse breeds in Iran: Arabian, Thoroughbred, KWPN, Caspian, Kurdish, and Turkmen. The goal was to identify differences in <i>ACOX1</i> genotype distribution, potentially serving as genetic markers under selection pressure related to breed-specific traits. In a sample of 324 horses, genomic DNA was analyzed using PCR-RFLP, revealing three genotypes (TT, TG, GG). The GG genotype was most common in Kurdish and Arabian horses (86% and 70%, respectively), while the TT genotype was prevalent in Turkmen (24%) and Thoroughbred horses (23%). The T allele’s frequency in Thoroughbred and Turkmen horses suggests that ACOX1 may be under selection pressure for phenotypic traits. Differences in genotype distribution were confirmed among breeds, with no sex-based association. The study concludes that <i>ACOX1</i> is a potential genetic marker for horse performance and adaptability, emphasizing the importance of genetic diversity in breeding programs. |
| format | Article |
| id | doaj-art-e76124ea666245b9ab6eb39a6586f5e4 |
| institution | OA Journals |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-e76124ea666245b9ab6eb39a6586f5e42025-08-20T02:00:51ZengMDPI AGAnimals2076-26152024-12-011424356610.3390/ani14243566Study of Variation of <i>ACOX1</i> Gene Among Different Horse Breeds Maintained in IranShayan Boozarjomehri Amnieh0Ali Hassanpour1Sina Moghaddam2Fatemeh Sakhaee3Katarzyna Ropka-Molik4Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz 5159115705, IranDepartment of Clinical Science, Tabriz Medical Sciences, Islamic Azad University, Tabriz 5159115705, IranDepartment of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran 1417935840, IranDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran 1963737611, IranDepartment of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandThe <i>ACOX1</i> gene is vital for fatty acid metabolism and is linked to environmental stress and physical exertion adaptation. The p.Asp237Ser variant (rs782885985) in <i>ACOX1</i> is associated with increased enzyme activity and reactive oxygen species (ROS) levels. This study examined the <i>ACOX1</i> polymorphism across six horse breeds in Iran: Arabian, Thoroughbred, KWPN, Caspian, Kurdish, and Turkmen. The goal was to identify differences in <i>ACOX1</i> genotype distribution, potentially serving as genetic markers under selection pressure related to breed-specific traits. In a sample of 324 horses, genomic DNA was analyzed using PCR-RFLP, revealing three genotypes (TT, TG, GG). The GG genotype was most common in Kurdish and Arabian horses (86% and 70%, respectively), while the TT genotype was prevalent in Turkmen (24%) and Thoroughbred horses (23%). The T allele’s frequency in Thoroughbred and Turkmen horses suggests that ACOX1 may be under selection pressure for phenotypic traits. Differences in genotype distribution were confirmed among breeds, with no sex-based association. The study concludes that <i>ACOX1</i> is a potential genetic marker for horse performance and adaptability, emphasizing the importance of genetic diversity in breeding programs.https://www.mdpi.com/2076-2615/14/24/3566Arabian horsesThoroughbredKWPNCaspianKurdishTurkmen |
| spellingShingle | Shayan Boozarjomehri Amnieh Ali Hassanpour Sina Moghaddam Fatemeh Sakhaee Katarzyna Ropka-Molik Study of Variation of <i>ACOX1</i> Gene Among Different Horse Breeds Maintained in Iran Animals Arabian horses Thoroughbred KWPN Caspian Kurdish Turkmen |
| title | Study of Variation of <i>ACOX1</i> Gene Among Different Horse Breeds Maintained in Iran |
| title_full | Study of Variation of <i>ACOX1</i> Gene Among Different Horse Breeds Maintained in Iran |
| title_fullStr | Study of Variation of <i>ACOX1</i> Gene Among Different Horse Breeds Maintained in Iran |
| title_full_unstemmed | Study of Variation of <i>ACOX1</i> Gene Among Different Horse Breeds Maintained in Iran |
| title_short | Study of Variation of <i>ACOX1</i> Gene Among Different Horse Breeds Maintained in Iran |
| title_sort | study of variation of i acox1 i gene among different horse breeds maintained in iran |
| topic | Arabian horses Thoroughbred KWPN Caspian Kurdish Turkmen |
| url | https://www.mdpi.com/2076-2615/14/24/3566 |
| work_keys_str_mv | AT shayanboozarjomehriamnieh studyofvariationofiacox1igeneamongdifferenthorsebreedsmaintainediniran AT alihassanpour studyofvariationofiacox1igeneamongdifferenthorsebreedsmaintainediniran AT sinamoghaddam studyofvariationofiacox1igeneamongdifferenthorsebreedsmaintainediniran AT fatemehsakhaee studyofvariationofiacox1igeneamongdifferenthorsebreedsmaintainediniran AT katarzynaropkamolik studyofvariationofiacox1igeneamongdifferenthorsebreedsmaintainediniran |