In vivo and In vitro Biocompatibility Study of Fe3O4@ZnO and Fe3O4@SiO2 as Photosensitizer for Targeted Breast Cancer Drug Delivery
The present study aimed to prepare Fe3O4 nanocarriers (NCs) by a thermal treatment method. After the Fe3O4 (Fe) NCs was prepared, zinc oxide and silica nanoparticles were added to it as Photosensitizer. The structure, morphology, and magnetic properties of Fe3O4@ZnO (Fe@Zn) and Fe3O4@SiO2 (Fe@Si) NC...
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University of Tehran
2020-11-01
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| Series: | Journal of Sciences, Islamic Republic of Iran |
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| Online Access: | https://jsciences.ut.ac.ir/article_78695_1e7832e2d656ca2077b1c8b2c6a5ae22.pdf |
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| author | ehsan naderi Mahmoud Naseri Heidar Taimouri Rad Reza Zolfaghari Emameh Gholamreza Farnoosh Ramezan Ali Taheri |
| author_facet | ehsan naderi Mahmoud Naseri Heidar Taimouri Rad Reza Zolfaghari Emameh Gholamreza Farnoosh Ramezan Ali Taheri |
| author_sort | ehsan naderi |
| collection | DOAJ |
| description | The present study aimed to prepare Fe3O4 nanocarriers (NCs) by a thermal treatment method. After the Fe3O4 (Fe) NCs was prepared, zinc oxide and silica nanoparticles were added to it as Photosensitizer. The structure, morphology, and magnetic properties of Fe3O4@ZnO (Fe@Zn) and Fe3O4@SiO2 (Fe@Si) NCs were determined by XRD, FT-IR, FESEM, and VSM. Then, the loading and drug release of Fe, Fe@Zn, and Fe@Si NCs were investigated. The curcumin (CUR) release of Fe@Zn+CUR and Fe@Si+CUR increased from 30% and 26% at pH 7.4 to 53% and 57% at pH 5.5, respectively. The cytotoxicity of Fe@Zn and Fe@Si NCs were determined by MTT assay, hemolysis test, acute toxicity, and lethal dose test. The results showed that Fe@Zn and Fe@Si were appropriate for Photodynamic Therapy (PDT) and in the next step, the effect of Fe@Zn, Fe@Si, Fe@Zn+CUR, and Fe@Si+CUR NCs on MCF-7 cells under visible light were studied. Finally, the ranking of the destruction of cancerous cells of MCF-7 using NCs under visible light was: Fe@Zn+CUR>Fe@Zn>Fe@Si+CUR>Fe@Si. |
| format | Article |
| id | doaj-art-e755d1285ae745a589c9596432d7bc2e |
| institution | OA Journals |
| issn | 1016-1104 2345-6914 |
| language | English |
| publishDate | 2020-11-01 |
| publisher | University of Tehran |
| record_format | Article |
| series | Journal of Sciences, Islamic Republic of Iran |
| spelling | doaj-art-e755d1285ae745a589c9596432d7bc2e2025-08-20T02:25:54ZengUniversity of TehranJournal of Sciences, Islamic Republic of Iran1016-11042345-69142020-11-0131435736810.22059/jsciences.2020.308691.100755978695In vivo and In vitro Biocompatibility Study of Fe3O4@ZnO and Fe3O4@SiO2 as Photosensitizer for Targeted Breast Cancer Drug Deliveryehsan naderi0Mahmoud Naseri1Heidar Taimouri Rad2Reza Zolfaghari Emameh3Gholamreza Farnoosh4Ramezan Ali Taheri5Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Islamic Republic of IranDepartment of Physics, Malayer University, Malayer, Iran.Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Islamic Republic of IranDepartment of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), 14965/161, Tehran, Islamic Republic of IranBiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Islamic Republic of IranNanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Islamic Republic of IranThe present study aimed to prepare Fe3O4 nanocarriers (NCs) by a thermal treatment method. After the Fe3O4 (Fe) NCs was prepared, zinc oxide and silica nanoparticles were added to it as Photosensitizer. The structure, morphology, and magnetic properties of Fe3O4@ZnO (Fe@Zn) and Fe3O4@SiO2 (Fe@Si) NCs were determined by XRD, FT-IR, FESEM, and VSM. Then, the loading and drug release of Fe, Fe@Zn, and Fe@Si NCs were investigated. The curcumin (CUR) release of Fe@Zn+CUR and Fe@Si+CUR increased from 30% and 26% at pH 7.4 to 53% and 57% at pH 5.5, respectively. The cytotoxicity of Fe@Zn and Fe@Si NCs were determined by MTT assay, hemolysis test, acute toxicity, and lethal dose test. The results showed that Fe@Zn and Fe@Si were appropriate for Photodynamic Therapy (PDT) and in the next step, the effect of Fe@Zn, Fe@Si, Fe@Zn+CUR, and Fe@Si+CUR NCs on MCF-7 cells under visible light were studied. Finally, the ranking of the destruction of cancerous cells of MCF-7 using NCs under visible light was: Fe@Zn+CUR>Fe@Zn>Fe@Si+CUR>Fe@Si.https://jsciences.ut.ac.ir/article_78695_1e7832e2d656ca2077b1c8b2c6a5ae22.pdfphotosensitizerdrug deliverycurcumincytotoxicitymagnetic properties |
| spellingShingle | ehsan naderi Mahmoud Naseri Heidar Taimouri Rad Reza Zolfaghari Emameh Gholamreza Farnoosh Ramezan Ali Taheri In vivo and In vitro Biocompatibility Study of Fe3O4@ZnO and Fe3O4@SiO2 as Photosensitizer for Targeted Breast Cancer Drug Delivery Journal of Sciences, Islamic Republic of Iran photosensitizer drug delivery curcumin cytotoxicity magnetic properties |
| title | In vivo and In vitro Biocompatibility Study of Fe3O4@ZnO and Fe3O4@SiO2 as Photosensitizer for Targeted Breast Cancer Drug Delivery |
| title_full | In vivo and In vitro Biocompatibility Study of Fe3O4@ZnO and Fe3O4@SiO2 as Photosensitizer for Targeted Breast Cancer Drug Delivery |
| title_fullStr | In vivo and In vitro Biocompatibility Study of Fe3O4@ZnO and Fe3O4@SiO2 as Photosensitizer for Targeted Breast Cancer Drug Delivery |
| title_full_unstemmed | In vivo and In vitro Biocompatibility Study of Fe3O4@ZnO and Fe3O4@SiO2 as Photosensitizer for Targeted Breast Cancer Drug Delivery |
| title_short | In vivo and In vitro Biocompatibility Study of Fe3O4@ZnO and Fe3O4@SiO2 as Photosensitizer for Targeted Breast Cancer Drug Delivery |
| title_sort | in vivo and in vitro biocompatibility study of fe3o4 zno and fe3o4 sio2 as photosensitizer for targeted breast cancer drug delivery |
| topic | photosensitizer drug delivery curcumin cytotoxicity magnetic properties |
| url | https://jsciences.ut.ac.ir/article_78695_1e7832e2d656ca2077b1c8b2c6a5ae22.pdf |
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