RSCUcaller: an R package for analyzing differences in relative synonymous codon usage (RSCU)
Abstract Background Synonymous codon usage bias, a significant factor in gene expression and genome evolution, was extensively studied in genomics and molecular biology. Although the genetic code is universal, significant variations in synonymous codon usage have been observed among and within organ...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-05-01
|
| Series: | BMC Bioinformatics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12859-025-06166-5 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849687982303346688 |
|---|---|
| author | Mateusz Maździarz Sebastian Zając Łukasz Paukszto Jakub Sawicki |
| author_facet | Mateusz Maździarz Sebastian Zając Łukasz Paukszto Jakub Sawicki |
| author_sort | Mateusz Maździarz |
| collection | DOAJ |
| description | Abstract Background Synonymous codon usage bias, a significant factor in gene expression and genome evolution, was extensively studied in genomics and molecular biology. Although the genetic code is universal, significant variations in synonymous codon usage have been observed among and within organisms. This bias was linked to various factors, including gene expression levels, tRNA abundance, protein structure, and environmental adaptation. Relative Synonymous Codon Usage (RSCU), a normalized measure, was used to quantify this bias. By analyzing RSCU values, researchers uncovered patterns and trends related to the underlying mechanisms driving codon usage bias. Results We present an R package named RSCUcaller designed for the analysis of coding nucleotide sequences at the level of relative synonymous codon usage (RSCU). The package enables both visualization of data and the performance of advanced statistical analyses. RSCUcaller accepts as input a multi-fasta file containing coding sequences (CDS) and an accompanying description table. Alternatively, the user may provide separate fasta files for each sequence along with the corresponding table. The program merges the provided sequences and calculates RSCU values for each. Implemented visualization features include creating heatmaps and dendrograms based on these heatmaps. Furthermore, the package allows for the presentation of data in the form of histograms. The calculated RSCU values can also be used to create matrices that can be subjected to further analysis by the user. RSCUcaller offers the functionality of correlation analysis between any two organisms. Additionally, to compare the frequency of amino acid occurrence between different groups of sequences, statistical tests have been implemented. Conclusions RSCUcaller enabled comparative RSCU analysis between coding sequences of different organisms or individuals of the same species. It facilitated visualization and statistical analysis among codons and user-defined groups. The RSCUcaller package is available at https://github.com/Mordziarz/RSCUcaller under the GPL-3 license. |
| format | Article |
| id | doaj-art-e753927504ec4fed8b0e74638c721863 |
| institution | DOAJ |
| issn | 1471-2105 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Bioinformatics |
| spelling | doaj-art-e753927504ec4fed8b0e74638c7218632025-08-20T03:22:11ZengBMCBMC Bioinformatics1471-21052025-05-0126111210.1186/s12859-025-06166-5RSCUcaller: an R package for analyzing differences in relative synonymous codon usage (RSCU)Mateusz Maździarz0Sebastian Zając1Łukasz Paukszto2Jakub Sawicki3Department of Botany and Evolutionary Ecology, University of Warmia and Mazury in OlsztynDecision Analysis and Support Unit, SGH Warsaw School of EconomicsDepartment of Botany and Evolutionary Ecology, University of Warmia and Mazury in OlsztynDepartment of Botany and Evolutionary Ecology, University of Warmia and Mazury in OlsztynAbstract Background Synonymous codon usage bias, a significant factor in gene expression and genome evolution, was extensively studied in genomics and molecular biology. Although the genetic code is universal, significant variations in synonymous codon usage have been observed among and within organisms. This bias was linked to various factors, including gene expression levels, tRNA abundance, protein structure, and environmental adaptation. Relative Synonymous Codon Usage (RSCU), a normalized measure, was used to quantify this bias. By analyzing RSCU values, researchers uncovered patterns and trends related to the underlying mechanisms driving codon usage bias. Results We present an R package named RSCUcaller designed for the analysis of coding nucleotide sequences at the level of relative synonymous codon usage (RSCU). The package enables both visualization of data and the performance of advanced statistical analyses. RSCUcaller accepts as input a multi-fasta file containing coding sequences (CDS) and an accompanying description table. Alternatively, the user may provide separate fasta files for each sequence along with the corresponding table. The program merges the provided sequences and calculates RSCU values for each. Implemented visualization features include creating heatmaps and dendrograms based on these heatmaps. Furthermore, the package allows for the presentation of data in the form of histograms. The calculated RSCU values can also be used to create matrices that can be subjected to further analysis by the user. RSCUcaller offers the functionality of correlation analysis between any two organisms. Additionally, to compare the frequency of amino acid occurrence between different groups of sequences, statistical tests have been implemented. Conclusions RSCUcaller enabled comparative RSCU analysis between coding sequences of different organisms or individuals of the same species. It facilitated visualization and statistical analysis among codons and user-defined groups. The RSCUcaller package is available at https://github.com/Mordziarz/RSCUcaller under the GPL-3 license.https://doi.org/10.1186/s12859-025-06166-5Codon usagePhylogeneticsDiversityCodons |
| spellingShingle | Mateusz Maździarz Sebastian Zając Łukasz Paukszto Jakub Sawicki RSCUcaller: an R package for analyzing differences in relative synonymous codon usage (RSCU) BMC Bioinformatics Codon usage Phylogenetics Diversity Codons |
| title | RSCUcaller: an R package for analyzing differences in relative synonymous codon usage (RSCU) |
| title_full | RSCUcaller: an R package for analyzing differences in relative synonymous codon usage (RSCU) |
| title_fullStr | RSCUcaller: an R package for analyzing differences in relative synonymous codon usage (RSCU) |
| title_full_unstemmed | RSCUcaller: an R package for analyzing differences in relative synonymous codon usage (RSCU) |
| title_short | RSCUcaller: an R package for analyzing differences in relative synonymous codon usage (RSCU) |
| title_sort | rscucaller an r package for analyzing differences in relative synonymous codon usage rscu |
| topic | Codon usage Phylogenetics Diversity Codons |
| url | https://doi.org/10.1186/s12859-025-06166-5 |
| work_keys_str_mv | AT mateuszmazdziarz rscucalleranrpackageforanalyzingdifferencesinrelativesynonymouscodonusagerscu AT sebastianzajac rscucalleranrpackageforanalyzingdifferencesinrelativesynonymouscodonusagerscu AT łukaszpaukszto rscucalleranrpackageforanalyzingdifferencesinrelativesynonymouscodonusagerscu AT jakubsawicki rscucalleranrpackageforanalyzingdifferencesinrelativesynonymouscodonusagerscu |