Role of caveolae in smooth muscle cells-mediated enhanced vasoconstriction of interlobar renal artery to angiotensin Ⅱ in SHR rats
Objective To investigate the role of caveolae in higher vasoconstriction of interlobar renal artery(IRA) of spontaneously hypertensive rat(SHR) to angiotensin Ⅱ(Ang Ⅱ).Methods Isometric force recording system was used to detect the vascular function.Methyl-beta-cyclodextrin(MCD) was employed to redu...
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Editorial Department of Journal of Clinical Nephrology
2016-01-01
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| Series: | Linchuang shenzangbing zazhi |
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| author | ZHANG Hai-jun ZHANG Xi XIE Xiao-ping CHANG Yao-ming BAO Jun-xiang |
| author_facet | ZHANG Hai-jun ZHANG Xi XIE Xiao-ping CHANG Yao-ming BAO Jun-xiang |
| author_sort | ZHANG Hai-jun |
| collection | DOAJ |
| description | Objective To investigate the role of caveolae in higher vasoconstriction of interlobar renal artery(IRA) of spontaneously hypertensive rat(SHR) to angiotensin Ⅱ(Ang Ⅱ).Methods Isometric force recording system was used to detect the vascular function.Methyl-beta-cyclodextrin(MCD) was employed to reduce the caveolae abundance.Real-time polymerase chain reaction(RTPCR) and Western blotting were conducted to examine the expression of mRNA and protein of angiotensin type 1 receptor(AT1R).Immumoprecipitation followed by Western blotting was carried out to test the binding of caveolin-1 and AT1 R.Results As compared to Wistar Kyoto(WKY) rats,the constriction of IRA to Ang Ⅱ was increased by 32.7%in SHR(P<0.05).Meanwhile,the abundance of caveolae on vascular smooth muscle cells(VSMCs) was enhanced significantly(P<0.05).After reducing the caveolae by MCD,the intensity and sensitivity of vasoconstriction to Ang Ⅱ in IRA of SHR were partly and entirely reversed,respectively.Moreover,AT1 R protein expression was higher in IRA of SHR group than in WKY group in which the AT1 R protein expression was lowered in part by MCD incubation.In IRA of both WKY group and SHR group,Ang Ⅱ could promote the binding of AT1 R to caveolin-1(P<0.05),which was by a larger extent in SHR group than in WKY group(P<0.05).After treatment with MCD,the binding of the two proteins in IRA of SHR group was restored to the level of WKY rats.Conclusions Caveolae regulates the enhanced intensity and sensitivity of vasoconstriction to Ang Ⅱ in IRA of SHR by raising AT1 R protein content and binding of AT1 R to caveolin-1. |
| format | Article |
| id | doaj-art-e7515da79aa94a2fae82718e3a68d660 |
| institution | OA Journals |
| issn | 1671-2390 |
| language | zho |
| publishDate | 2016-01-01 |
| publisher | Editorial Department of Journal of Clinical Nephrology |
| record_format | Article |
| series | Linchuang shenzangbing zazhi |
| spelling | doaj-art-e7515da79aa94a2fae82718e3a68d6602025-08-20T02:28:51ZzhoEditorial Department of Journal of Clinical NephrologyLinchuang shenzangbing zazhi1671-23902016-01-0162563157918354Role of caveolae in smooth muscle cells-mediated enhanced vasoconstriction of interlobar renal artery to angiotensin Ⅱ in SHR ratsZHANG Hai-junZHANG XiXIE Xiao-pingCHANG Yao-mingBAO Jun-xiangObjective To investigate the role of caveolae in higher vasoconstriction of interlobar renal artery(IRA) of spontaneously hypertensive rat(SHR) to angiotensin Ⅱ(Ang Ⅱ).Methods Isometric force recording system was used to detect the vascular function.Methyl-beta-cyclodextrin(MCD) was employed to reduce the caveolae abundance.Real-time polymerase chain reaction(RTPCR) and Western blotting were conducted to examine the expression of mRNA and protein of angiotensin type 1 receptor(AT1R).Immumoprecipitation followed by Western blotting was carried out to test the binding of caveolin-1 and AT1 R.Results As compared to Wistar Kyoto(WKY) rats,the constriction of IRA to Ang Ⅱ was increased by 32.7%in SHR(P<0.05).Meanwhile,the abundance of caveolae on vascular smooth muscle cells(VSMCs) was enhanced significantly(P<0.05).After reducing the caveolae by MCD,the intensity and sensitivity of vasoconstriction to Ang Ⅱ in IRA of SHR were partly and entirely reversed,respectively.Moreover,AT1 R protein expression was higher in IRA of SHR group than in WKY group in which the AT1 R protein expression was lowered in part by MCD incubation.In IRA of both WKY group and SHR group,Ang Ⅱ could promote the binding of AT1 R to caveolin-1(P<0.05),which was by a larger extent in SHR group than in WKY group(P<0.05).After treatment with MCD,the binding of the two proteins in IRA of SHR group was restored to the level of WKY rats.Conclusions Caveolae regulates the enhanced intensity and sensitivity of vasoconstriction to Ang Ⅱ in IRA of SHR by raising AT1 R protein content and binding of AT1 R to caveolin-1.http://www.lcszb.com/thesisDetails?columnId=57918354&Fpath=home&index=0Spontaneously hypertensive ratCaveolaeRenal arteryVasoconstrictionAngiotensin Ⅱ |
| spellingShingle | ZHANG Hai-jun ZHANG Xi XIE Xiao-ping CHANG Yao-ming BAO Jun-xiang Role of caveolae in smooth muscle cells-mediated enhanced vasoconstriction of interlobar renal artery to angiotensin Ⅱ in SHR rats Linchuang shenzangbing zazhi Spontaneously hypertensive rat Caveolae Renal artery Vasoconstriction Angiotensin Ⅱ |
| title | Role of caveolae in smooth muscle cells-mediated enhanced vasoconstriction of interlobar renal artery to angiotensin Ⅱ in SHR rats |
| title_full | Role of caveolae in smooth muscle cells-mediated enhanced vasoconstriction of interlobar renal artery to angiotensin Ⅱ in SHR rats |
| title_fullStr | Role of caveolae in smooth muscle cells-mediated enhanced vasoconstriction of interlobar renal artery to angiotensin Ⅱ in SHR rats |
| title_full_unstemmed | Role of caveolae in smooth muscle cells-mediated enhanced vasoconstriction of interlobar renal artery to angiotensin Ⅱ in SHR rats |
| title_short | Role of caveolae in smooth muscle cells-mediated enhanced vasoconstriction of interlobar renal artery to angiotensin Ⅱ in SHR rats |
| title_sort | role of caveolae in smooth muscle cells mediated enhanced vasoconstriction of interlobar renal artery to angiotensin ii in shr rats |
| topic | Spontaneously hypertensive rat Caveolae Renal artery Vasoconstriction Angiotensin Ⅱ |
| url | http://www.lcszb.com/thesisDetails?columnId=57918354&Fpath=home&index=0 |
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