The Isolation, Identification and Characterization of a Wild-Type Strain <i>Pseudomonas aeruginosa</i> PM1012 from the Cloacal Microbiota of a Common Wall Lizard (<i>Podarcis muralis</i> Laurenti, 1768)
<i>Pseudomonas aeruginosa</i> is a ubiquitous environmental Gram-negative bacterium and also an opportunistic pathogen for both humans and animals, causing acute or chronic infections. It has been frequently detected in healthy and diseased reptiles, more commonly in captive ones. Since...
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2024-09-01
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| author | Irina Lazarkevich Stephan Engibarov Simona Mitova Rumyana Eneva Tsvetelina Paunova-Krasteva Dayana Borisova Emiliya Vacheva Nikola Stanchev Steliyana Popova Yana Gocheva Maria Gerginova |
| author_facet | Irina Lazarkevich Stephan Engibarov Simona Mitova Rumyana Eneva Tsvetelina Paunova-Krasteva Dayana Borisova Emiliya Vacheva Nikola Stanchev Steliyana Popova Yana Gocheva Maria Gerginova |
| author_sort | Irina Lazarkevich |
| collection | DOAJ |
| description | <i>Pseudomonas aeruginosa</i> is a ubiquitous environmental Gram-negative bacterium and also an opportunistic pathogen for both humans and animals, causing acute or chronic infections. It has been frequently detected in healthy and diseased reptiles, more commonly in captive ones. Since most studies are primarily on clinical isolates, the pathogenic potential of strains originating from wild animals is poorly explored. We isolated the strain <i>P. aeruginosa</i> PM1012 from the cloacal microbiota of a common wall lizard (<i>Podarcis muralis</i> Laurenti, 1768) from a free-living population. The effect of temperature, pH and salinity on its growth was evaluated. Antibiotic resistance, the expression of several virulence factors as some extracellular enzymes, pyocyanin production and biofilm formation were also assessed. Apart from intrinsic resistance, the newly isolated strain <i>P. aeruginosa</i> PM1012 presented an antibiotic susceptibility profile with a low resistance rate limited to meropenem and intermediate to ceftazidime and aztreonam. Protease, lipase and gelatinase secretion was detected. Strong pyocyanin production was observed in the optimal range of growth conditions. An excellent biofilm-forming capacity was manifested. |
| format | Article |
| id | doaj-art-e7505a6f2b624d73ae6609afaef0ed09 |
| institution | OA Journals |
| issn | 2673-8007 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
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| series | Applied Microbiology |
| spelling | doaj-art-e7505a6f2b624d73ae6609afaef0ed092025-08-20T01:55:58ZengMDPI AGApplied Microbiology2673-80072024-09-01431396141010.3390/applmicrobiol4030096The Isolation, Identification and Characterization of a Wild-Type Strain <i>Pseudomonas aeruginosa</i> PM1012 from the Cloacal Microbiota of a Common Wall Lizard (<i>Podarcis muralis</i> Laurenti, 1768)Irina Lazarkevich0Stephan Engibarov1Simona Mitova2Rumyana Eneva3Tsvetelina Paunova-Krasteva4Dayana Borisova5Emiliya Vacheva6Nikola Stanchev7Steliyana Popova8Yana Gocheva9Maria Gerginova10The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Academician Georgi Bonchev Street, Building. 26, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Academician Georgi Bonchev Street, Building. 26, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Academician Georgi Bonchev Street, Building. 26, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Academician Georgi Bonchev Street, Building. 26, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Academician Georgi Bonchev Street, Building. 26, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Academician Georgi Bonchev Street, Building. 26, 1113 Sofia, BulgariaInstitute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 1 Tsar Osvoboditel Boulevard, 1000 Sofia, BulgariaFaculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Boulevard, 1164 Sofia, BulgariaFaculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Boulevard, 1164 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Academician Georgi Bonchev Street, Building. 26, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Academician Georgi Bonchev Street, Building. 26, 1113 Sofia, Bulgaria<i>Pseudomonas aeruginosa</i> is a ubiquitous environmental Gram-negative bacterium and also an opportunistic pathogen for both humans and animals, causing acute or chronic infections. It has been frequently detected in healthy and diseased reptiles, more commonly in captive ones. Since most studies are primarily on clinical isolates, the pathogenic potential of strains originating from wild animals is poorly explored. We isolated the strain <i>P. aeruginosa</i> PM1012 from the cloacal microbiota of a common wall lizard (<i>Podarcis muralis</i> Laurenti, 1768) from a free-living population. The effect of temperature, pH and salinity on its growth was evaluated. Antibiotic resistance, the expression of several virulence factors as some extracellular enzymes, pyocyanin production and biofilm formation were also assessed. Apart from intrinsic resistance, the newly isolated strain <i>P. aeruginosa</i> PM1012 presented an antibiotic susceptibility profile with a low resistance rate limited to meropenem and intermediate to ceftazidime and aztreonam. Protease, lipase and gelatinase secretion was detected. Strong pyocyanin production was observed in the optimal range of growth conditions. An excellent biofilm-forming capacity was manifested.https://www.mdpi.com/2673-8007/4/3/96<i>Pseudomonas aeruginosa</i>antibiotic resistancevirulence factorspyocyaninbiofilm formationreptile cloacal microbiota |
| spellingShingle | Irina Lazarkevich Stephan Engibarov Simona Mitova Rumyana Eneva Tsvetelina Paunova-Krasteva Dayana Borisova Emiliya Vacheva Nikola Stanchev Steliyana Popova Yana Gocheva Maria Gerginova The Isolation, Identification and Characterization of a Wild-Type Strain <i>Pseudomonas aeruginosa</i> PM1012 from the Cloacal Microbiota of a Common Wall Lizard (<i>Podarcis muralis</i> Laurenti, 1768) Applied Microbiology <i>Pseudomonas aeruginosa</i> antibiotic resistance virulence factors pyocyanin biofilm formation reptile cloacal microbiota |
| title | The Isolation, Identification and Characterization of a Wild-Type Strain <i>Pseudomonas aeruginosa</i> PM1012 from the Cloacal Microbiota of a Common Wall Lizard (<i>Podarcis muralis</i> Laurenti, 1768) |
| title_full | The Isolation, Identification and Characterization of a Wild-Type Strain <i>Pseudomonas aeruginosa</i> PM1012 from the Cloacal Microbiota of a Common Wall Lizard (<i>Podarcis muralis</i> Laurenti, 1768) |
| title_fullStr | The Isolation, Identification and Characterization of a Wild-Type Strain <i>Pseudomonas aeruginosa</i> PM1012 from the Cloacal Microbiota of a Common Wall Lizard (<i>Podarcis muralis</i> Laurenti, 1768) |
| title_full_unstemmed | The Isolation, Identification and Characterization of a Wild-Type Strain <i>Pseudomonas aeruginosa</i> PM1012 from the Cloacal Microbiota of a Common Wall Lizard (<i>Podarcis muralis</i> Laurenti, 1768) |
| title_short | The Isolation, Identification and Characterization of a Wild-Type Strain <i>Pseudomonas aeruginosa</i> PM1012 from the Cloacal Microbiota of a Common Wall Lizard (<i>Podarcis muralis</i> Laurenti, 1768) |
| title_sort | isolation identification and characterization of a wild type strain i pseudomonas aeruginosa i pm1012 from the cloacal microbiota of a common wall lizard i podarcis muralis i laurenti 1768 |
| topic | <i>Pseudomonas aeruginosa</i> antibiotic resistance virulence factors pyocyanin biofilm formation reptile cloacal microbiota |
| url | https://www.mdpi.com/2673-8007/4/3/96 |
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