Rosmarinic acid attenuated inflammation and apoptosis in folic acid-induced renal injury: Role of FoxO3/ NFκB pathway
Objective(s): Rosmarinic acid (RA) is a herbal compound with various antioxidant and anti-inflammatory effects. This study aimed to explore the anti-inflammatory and anti-apoptotic properties of RA in folic acid-induced renal injury.Materials and Methods: Thirty-six male C57/BL6 mice were randomly d...
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Mashhad University of Medical Sciences
2025-03-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_25026_3334f128e248335e04dfedc90a2de742.pdf |
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| author | Maryam Mottaghi Fatemeh Heidari Tahereh Komeili Movahhed Akram Eidi Azam Moslehi |
| author_facet | Maryam Mottaghi Fatemeh Heidari Tahereh Komeili Movahhed Akram Eidi Azam Moslehi |
| author_sort | Maryam Mottaghi |
| collection | DOAJ |
| description | Objective(s): Rosmarinic acid (RA) is a herbal compound with various antioxidant and anti-inflammatory effects. This study aimed to explore the anti-inflammatory and anti-apoptotic properties of RA in folic acid-induced renal injury.Materials and Methods: Thirty-six male C57/BL6 mice were randomly divided into six groups (N=6): Control (received normal saline), NaHCO3 (received NaHCO3 as folic acid solvent), FA (received folic acid (FA)(IP) to induce renal injury), RA (received 100 mg/kg RA), RA50-FA (received 50 mg/kg RA solution after folic acid injection), and RA100-FA (received 100 mg/kg RA after folic acid injection). For ten days, the treatment groups received RA by gavage. The effects of RA were assessed using H & E staining, biochemical tests, western blotting, and ELISA in the kidney tissues of the mice. Real-time RT-PCR was also performed to evaluate the expression changes of renal genes.Results: Our data showed that treatment by RA led to the over-expression of FoxO3 (P<0.05) and decrease in NFκB levels (P<0.01 and P<0.05) and expression of TNFα (P<0.05) and IL6 (P<0.001 and P<0.01). Other evaluations showed a decrease in p53 (P<0.01 and P<0.001), Bax/Bcl-2 ratio expression (P<0.01 and P<0.05), and Caspase-3 level (P<0.01 and P<0.05) compared to the folic acid group. Histological and biochemical results also confirmed the attenuation of tissue damage.Conclusion: This study revealed that RA’s positive effects on folic acid-induced renal injury might result from the involvement of the FoxO3/NFκB pathway, thereby suppressing inflammation and apoptosis. |
| format | Article |
| id | doaj-art-e73fa9d6e9404e468022f24e759c4cba |
| institution | Kabale University |
| issn | 2008-3866 2008-3874 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj-art-e73fa9d6e9404e468022f24e759c4cba2025-08-20T03:56:45ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742025-03-0128331632210.22038/ijbms.2024.80551.1743625026Rosmarinic acid attenuated inflammation and apoptosis in folic acid-induced renal injury: Role of FoxO3/ NFκB pathwayMaryam Mottaghi0Fatemeh Heidari1Tahereh Komeili Movahhed2Akram Eidi3Azam Moslehi4Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranCellular and Molecular Research Center, Qom University of Medical Sciences, Qom, IranCellular and Molecular Research Center, Qom University of Medical Sciences, Qom, IranDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranCellular and Molecular Research Center, Qom University of Medical Sciences, Qom, IranObjective(s): Rosmarinic acid (RA) is a herbal compound with various antioxidant and anti-inflammatory effects. This study aimed to explore the anti-inflammatory and anti-apoptotic properties of RA in folic acid-induced renal injury.Materials and Methods: Thirty-six male C57/BL6 mice were randomly divided into six groups (N=6): Control (received normal saline), NaHCO3 (received NaHCO3 as folic acid solvent), FA (received folic acid (FA)(IP) to induce renal injury), RA (received 100 mg/kg RA), RA50-FA (received 50 mg/kg RA solution after folic acid injection), and RA100-FA (received 100 mg/kg RA after folic acid injection). For ten days, the treatment groups received RA by gavage. The effects of RA were assessed using H & E staining, biochemical tests, western blotting, and ELISA in the kidney tissues of the mice. Real-time RT-PCR was also performed to evaluate the expression changes of renal genes.Results: Our data showed that treatment by RA led to the over-expression of FoxO3 (P<0.05) and decrease in NFκB levels (P<0.01 and P<0.05) and expression of TNFα (P<0.05) and IL6 (P<0.001 and P<0.01). Other evaluations showed a decrease in p53 (P<0.01 and P<0.001), Bax/Bcl-2 ratio expression (P<0.01 and P<0.05), and Caspase-3 level (P<0.01 and P<0.05) compared to the folic acid group. Histological and biochemical results also confirmed the attenuation of tissue damage.Conclusion: This study revealed that RA’s positive effects on folic acid-induced renal injury might result from the involvement of the FoxO3/NFκB pathway, thereby suppressing inflammation and apoptosis.https://ijbms.mums.ac.ir/article_25026_3334f128e248335e04dfedc90a2de742.pdfapoptosisfolic acidinflammationkidney injuryrosmarinic acid |
| spellingShingle | Maryam Mottaghi Fatemeh Heidari Tahereh Komeili Movahhed Akram Eidi Azam Moslehi Rosmarinic acid attenuated inflammation and apoptosis in folic acid-induced renal injury: Role of FoxO3/ NFκB pathway Iranian Journal of Basic Medical Sciences apoptosis folic acid inflammation kidney injury rosmarinic acid |
| title | Rosmarinic acid attenuated inflammation and apoptosis in folic acid-induced renal injury: Role of FoxO3/ NFκB pathway |
| title_full | Rosmarinic acid attenuated inflammation and apoptosis in folic acid-induced renal injury: Role of FoxO3/ NFκB pathway |
| title_fullStr | Rosmarinic acid attenuated inflammation and apoptosis in folic acid-induced renal injury: Role of FoxO3/ NFκB pathway |
| title_full_unstemmed | Rosmarinic acid attenuated inflammation and apoptosis in folic acid-induced renal injury: Role of FoxO3/ NFκB pathway |
| title_short | Rosmarinic acid attenuated inflammation and apoptosis in folic acid-induced renal injury: Role of FoxO3/ NFκB pathway |
| title_sort | rosmarinic acid attenuated inflammation and apoptosis in folic acid induced renal injury role of foxo3 nfκb pathway |
| topic | apoptosis folic acid inflammation kidney injury rosmarinic acid |
| url | https://ijbms.mums.ac.ir/article_25026_3334f128e248335e04dfedc90a2de742.pdf |
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