Oryzalin-induced polyploidy in Vanda limbata (Blume): Phenotypic assessment
Background: Vanda limbata is a natural orchid species found on Java Island, commonly known as V. limbata 'Jawa.' Enhancing plant vigor is essential to increase its potential as an ornamental plant, with one promising approach being induced polyploidy using chemical mutagens such as oryzal...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Biochemistry and Molecular Biology
2025-01-01
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| Series: | Acta Biochimica Indonesiana |
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| Online Access: | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/197 |
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| _version_ | 1825199619874750464 |
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| author | Murni Dwiati Wahyu Nur Hasam Agus Hery Susanto |
| author_facet | Murni Dwiati Wahyu Nur Hasam Agus Hery Susanto |
| author_sort | Murni Dwiati |
| collection | DOAJ |
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Background: Vanda limbata is a natural orchid species found on Java Island, commonly known as V. limbata 'Jawa.' Enhancing plant vigor is essential to increase its potential as an ornamental plant, with one promising approach being induced polyploidy using chemical mutagens such as oryzalin.
Objective: This study aimed to evaluate the effect of oryzalin on inducing polyploidy in V. limbata cultured in vitro.
Method: Oryzalin was applied at concentrations of 0, 25, 50, 75, and 100 µM, with five replications for each treatment, resulting in 25 experimental units. Several morpho-physiological and anatomical traits were measured as indicators of polyploidy.
Results: The results demonstrated that oryzalin at 100 µM was the most effective concentration for inducing polyploidy in V. limbata cultured in vitro. This was particularly evident in traits such as reduced leaf length, increased leaf width, enhanced adventitious shoot formation, and enlarged stomatal width.
Conclusion: Oryzalin, when applied at appropriate concentrations, can be effectively used to induce polyploidy in V. limbate.
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| format | Article |
| id | doaj-art-e73f8196bf274257ac05d1f0287e8657 |
| institution | Kabale University |
| issn | 2654-6108 2654-3222 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Indonesian Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | Acta Biochimica Indonesiana |
| spelling | doaj-art-e73f8196bf274257ac05d1f0287e86572025-02-08T03:04:39ZengIndonesian Society for Biochemistry and Molecular BiologyActa Biochimica Indonesiana2654-61082654-32222025-01-018110.32889/actabioina.197Oryzalin-induced polyploidy in Vanda limbata (Blume): Phenotypic assessmentMurni Dwiati0Wahyu Nur Hasam1Agus Hery Susanto2Faculty of Biology Universitas Jenderal Soediman, Jl. dr. Suparno No. 63 Purwokerto 53122, IndonesiaFaculty of Biology Universitas Jenderal Soediman, Jl. dr. Suparno No. 63 Purwokerto 53122, IndonesiaFaculty of Biology Universitas Jenderal Soediman, Jl. dr. Suparno No. 63 Purwokerto 53122, Indonesia Background: Vanda limbata is a natural orchid species found on Java Island, commonly known as V. limbata 'Jawa.' Enhancing plant vigor is essential to increase its potential as an ornamental plant, with one promising approach being induced polyploidy using chemical mutagens such as oryzalin. Objective: This study aimed to evaluate the effect of oryzalin on inducing polyploidy in V. limbata cultured in vitro. Method: Oryzalin was applied at concentrations of 0, 25, 50, 75, and 100 µM, with five replications for each treatment, resulting in 25 experimental units. Several morpho-physiological and anatomical traits were measured as indicators of polyploidy. Results: The results demonstrated that oryzalin at 100 µM was the most effective concentration for inducing polyploidy in V. limbata cultured in vitro. This was particularly evident in traits such as reduced leaf length, increased leaf width, enhanced adventitious shoot formation, and enlarged stomatal width. Conclusion: Oryzalin, when applied at appropriate concentrations, can be effectively used to induce polyploidy in V. limbate. https://pbbmi.org/newjurnal/index.php/actabioina/article/view/197in vitro cultureoryzalinpolyploidizationVanda limbata |
| spellingShingle | Murni Dwiati Wahyu Nur Hasam Agus Hery Susanto Oryzalin-induced polyploidy in Vanda limbata (Blume): Phenotypic assessment Acta Biochimica Indonesiana in vitro culture oryzalin polyploidization Vanda limbata |
| title | Oryzalin-induced polyploidy in Vanda limbata (Blume): Phenotypic assessment |
| title_full | Oryzalin-induced polyploidy in Vanda limbata (Blume): Phenotypic assessment |
| title_fullStr | Oryzalin-induced polyploidy in Vanda limbata (Blume): Phenotypic assessment |
| title_full_unstemmed | Oryzalin-induced polyploidy in Vanda limbata (Blume): Phenotypic assessment |
| title_short | Oryzalin-induced polyploidy in Vanda limbata (Blume): Phenotypic assessment |
| title_sort | oryzalin induced polyploidy in vanda limbata blume phenotypic assessment |
| topic | in vitro culture oryzalin polyploidization Vanda limbata |
| url | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/197 |
| work_keys_str_mv | AT murnidwiati oryzalininducedpolyploidyinvandalimbatablumephenotypicassessment AT wahyunurhasam oryzalininducedpolyploidyinvandalimbatablumephenotypicassessment AT agusherysusanto oryzalininducedpolyploidyinvandalimbatablumephenotypicassessment |