Proteomic analysis of outer membrane vesicles derived from the type A5 Strain of Mannheimia haemolytica
Mannheimia haemolytica (M. haemolytica) cause mastitis in sheep, acute sepsis in newborn lambs, and co-infections with various pathogens, leading to bovine respiratory disease syndrome (BRDS), these infections have resulted in significant economic losses to both domestic and international farming in...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1578027/full |
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| author | Ke Shang Ke Shang Ke Shang Yuanji Gao Yuanji Gao Jiangbo Du Chang Liu Chang Liu Jinglei Dai Jinglei Dai Junfeng Zhang Junfeng Zhang Yanyan Jia Yanyan Jia Zuhua Yu Zuhua Yu Songbiao Chen Songbiao Chen Zhongyu Liu |
| author_facet | Ke Shang Ke Shang Ke Shang Yuanji Gao Yuanji Gao Jiangbo Du Chang Liu Chang Liu Jinglei Dai Jinglei Dai Junfeng Zhang Junfeng Zhang Yanyan Jia Yanyan Jia Zuhua Yu Zuhua Yu Songbiao Chen Songbiao Chen Zhongyu Liu |
| author_sort | Ke Shang |
| collection | DOAJ |
| description | Mannheimia haemolytica (M. haemolytica) cause mastitis in sheep, acute sepsis in newborn lambs, and co-infections with various pathogens, leading to bovine respiratory disease syndrome (BRDS), these infections have resulted in significant economic losses to both domestic and international farming industries. An in-depth understanding of the pathogenic mechanisms of M. haemolytica is crucial for the prevention and control of this disease. Outer membrane vesicles (OMVs) play a vital role in bacterial pathogenesis, serving as key mediators of interactions between Gram-negative bacteria and their hosts. However, the specific role of OMVs in the pathogenic process of M. haemolytica remains poorly understood. To address this, we isolated OMVs from the Mannheimia haemolytica Type A5 strain (MH-5) using ultracentrifugation and subsequently characterized their secretory properties, protein composition, and immunogenicity through electron microscopy, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and cellular experiments. The electron microscopy results indicated that the MH-5 strain secreted OMVs under natural growth conditions. Proteomic and bioinformatics analyses revealed that these OMVs contained 282 proteins, with significant enrichment in proteins related to immunity, iron metabolism, and catalytic activity. Cellular experiments demonstrated that, compared to the control group, the OMVs group exhibited a significant increase in the mRNA expression of IL-1β, IL-6, and TNF-α, with secretion levels increasing in a dose-dependent manner, thereby enhancing the inflammatory response. These findings lay the groundwork for further exploration of the role of OMVs in the pathogenesis of M. haemolytica and provide insights for the development of effective vaccines and antibiotics against this pathogen. |
| format | Article |
| id | doaj-art-e73de5cdafa649b2a966373659bfe5ba |
| institution | OA Journals |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-e73de5cdafa649b2a966373659bfe5ba2025-08-20T02:22:36ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-06-011510.3389/fcimb.2025.15780271578027Proteomic analysis of outer membrane vesicles derived from the type A5 Strain of Mannheimia haemolyticaKe Shang0Ke Shang1Ke Shang2Yuanji Gao3Yuanji Gao4Jiangbo Du5Chang Liu6Chang Liu7Jinglei Dai8Jinglei Dai9Junfeng Zhang10Junfeng Zhang11Yanyan Jia12Yanyan Jia13Zuhua Yu14Zuhua Yu15Songbiao Chen16Songbiao Chen17Zhongyu Liu18The 989th Hospital of the Joint Logistics Support Force of Chinese People’s Liberation Army, Luoyang, ChinaLaboratory of Functional Microbiology and Animal Health, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Henan University of Science and Technology, Luoyang, ChinaLaboratory of Functional Microbiology and Animal Health, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Henan University of Science and Technology, Luoyang, ChinaThe 989th Hospital of the Joint Logistics Support Force of Chinese People’s Liberation Army, Luoyang, ChinaLaboratory of Functional Microbiology and Animal Health, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Henan University of Science and Technology, Luoyang, ChinaLaboratory of Functional Microbiology and Animal Health, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Henan University of Science and Technology, Luoyang, ChinaThe 989th Hospital of the Joint Logistics Support Force of Chinese People’s Liberation Army, Luoyang, ChinaCollege of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, ChinaLaboratory of Functional Microbiology and Animal Health, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Henan University of Science and Technology, Luoyang, ChinaLaboratory of Functional Microbiology and Animal Health, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Henan University of Science and Technology, Luoyang, ChinaLaboratory of Functional Microbiology and Animal Health, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaLuoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Henan University of Science and Technology, Luoyang, ChinaThe 989th Hospital of the Joint Logistics Support Force of Chinese People’s Liberation Army, Luoyang, ChinaMannheimia haemolytica (M. haemolytica) cause mastitis in sheep, acute sepsis in newborn lambs, and co-infections with various pathogens, leading to bovine respiratory disease syndrome (BRDS), these infections have resulted in significant economic losses to both domestic and international farming industries. An in-depth understanding of the pathogenic mechanisms of M. haemolytica is crucial for the prevention and control of this disease. Outer membrane vesicles (OMVs) play a vital role in bacterial pathogenesis, serving as key mediators of interactions between Gram-negative bacteria and their hosts. However, the specific role of OMVs in the pathogenic process of M. haemolytica remains poorly understood. To address this, we isolated OMVs from the Mannheimia haemolytica Type A5 strain (MH-5) using ultracentrifugation and subsequently characterized their secretory properties, protein composition, and immunogenicity through electron microscopy, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and cellular experiments. The electron microscopy results indicated that the MH-5 strain secreted OMVs under natural growth conditions. Proteomic and bioinformatics analyses revealed that these OMVs contained 282 proteins, with significant enrichment in proteins related to immunity, iron metabolism, and catalytic activity. Cellular experiments demonstrated that, compared to the control group, the OMVs group exhibited a significant increase in the mRNA expression of IL-1β, IL-6, and TNF-α, with secretion levels increasing in a dose-dependent manner, thereby enhancing the inflammatory response. These findings lay the groundwork for further exploration of the role of OMVs in the pathogenesis of M. haemolytica and provide insights for the development of effective vaccines and antibiotics against this pathogen.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1578027/fullMannheimia haemolytica type A5outer membrane vesiclesproteomicsinflammatory responsein vitro test |
| spellingShingle | Ke Shang Ke Shang Ke Shang Yuanji Gao Yuanji Gao Jiangbo Du Chang Liu Chang Liu Jinglei Dai Jinglei Dai Junfeng Zhang Junfeng Zhang Yanyan Jia Yanyan Jia Zuhua Yu Zuhua Yu Songbiao Chen Songbiao Chen Zhongyu Liu Proteomic analysis of outer membrane vesicles derived from the type A5 Strain of Mannheimia haemolytica Frontiers in Cellular and Infection Microbiology Mannheimia haemolytica type A5 outer membrane vesicles proteomics inflammatory response in vitro test |
| title | Proteomic analysis of outer membrane vesicles derived from the type A5 Strain of Mannheimia haemolytica |
| title_full | Proteomic analysis of outer membrane vesicles derived from the type A5 Strain of Mannheimia haemolytica |
| title_fullStr | Proteomic analysis of outer membrane vesicles derived from the type A5 Strain of Mannheimia haemolytica |
| title_full_unstemmed | Proteomic analysis of outer membrane vesicles derived from the type A5 Strain of Mannheimia haemolytica |
| title_short | Proteomic analysis of outer membrane vesicles derived from the type A5 Strain of Mannheimia haemolytica |
| title_sort | proteomic analysis of outer membrane vesicles derived from the type a5 strain of mannheimia haemolytica |
| topic | Mannheimia haemolytica type A5 outer membrane vesicles proteomics inflammatory response in vitro test |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1578027/full |
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