Clinical significance of FOXN3 expression in Indian breast cancer patients
Abstract Globally, breast cancer is the most common cancer to affect women. There are different molecular and pathological factors that are involved in the uncontrolled proliferation of breast cancer cells. FOXN3 that is member of Forkhead box family proteins is well recognized for having a crucial...
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Nature Portfolio
2025-04-01
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| Online Access: | https://doi.org/10.1038/s41598-025-98090-0 |
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| author | Sheersh Massey Mohammad Aasif Khan Safia Obaidur Rab Syeda Maryam Husain Asifa Khan Sadaf Zoya Mallik Saad Mustafa Rahul Kumar Maria Habib S. V. S. Deo Syed Akhtar Husain |
| author_facet | Sheersh Massey Mohammad Aasif Khan Safia Obaidur Rab Syeda Maryam Husain Asifa Khan Sadaf Zoya Mallik Saad Mustafa Rahul Kumar Maria Habib S. V. S. Deo Syed Akhtar Husain |
| author_sort | Sheersh Massey |
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| description | Abstract Globally, breast cancer is the most common cancer to affect women. There are different molecular and pathological factors that are involved in the uncontrolled proliferation of breast cancer cells. FOXN3 that is member of Forkhead box family proteins is well recognized for having a crucial role in different biological processes and is reported to be dysregulated in various malignancies. The studies to evaluate the significance of the FOXN3 gene in progression of breast cancer are still under progress. We in the current study aim to examine the FOXN3 gene expression in Indian breast cancer patients and find its clinical relevance. FOXN3 expression analysis using RT-PCR, immunohistochemistry, and western blotting was performed in tumor and normal tissue collected from 142 sporadic breast cancer patients. To identify the genetic aberrations in FOXN3 gene automated DNA sequencing was done. FOXN3 expression study revealed the elevated expression of FOXN3 mRNA in 61.26% of the cases whereas FOXN3 protein was seen to be overexpressed in 59.15% cases. Further, it was found that the elevated expression of FOXN3 mRNA was significantly correlated with the post-menopausal (p = 0.003) status and positive lymph node status (p = 0.049) of the patients. The FOXN3 protein expression also exhibited the significant association with menopausal status (p = 0.008), lymph node status (p = 0.001) and clinical stage (p = 0.018) of the patients. However, we did not find any mutation in the DNA binding domain of the FOXN3 gene in the Indian breast cancer cases. Our findings indicates that overexpression of FOXN3 gene in Indian breast cancer cases can have a potential role in breast cancer progression especially in advanced clinical stages. |
| format | Article |
| id | doaj-art-e73d5cde643b444f8d22e943653f71d9 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-e73d5cde643b444f8d22e943653f71d92025-08-20T02:24:29ZengNature PortfolioScientific Reports2045-23222025-04-0115111110.1038/s41598-025-98090-0Clinical significance of FOXN3 expression in Indian breast cancer patientsSheersh Massey0Mohammad Aasif Khan1Safia Obaidur Rab2Syeda Maryam Husain3Asifa Khan4Sadaf5Zoya Mallik6Saad Mustafa7Rahul Kumar8Maria Habib9S. V. S. Deo10Syed Akhtar Husain11Human Genetics Laboratory, Department of Biosciences, Jamia Millia IslamiaThe University of Texas Health Science Center at San AntonioDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid UniversityAl-Falah School of Medical Science and Research CentreMolecular, Cell and Cancer Biology Department, UMass Chan Medical SchoolMedical Biotechnology Laboratory, Department of Biotechnology, Jamia Millia IslamiaHuman Genetics Laboratory, Department of Biosciences, Jamia Millia IslamiaDepartment of Geriatric Medicine, All India Institute of Medical Sciences (AIIMS)Department of Biotechnology GITAM School of Science, GITAMDDU KAUSHAL Kendra, Jamia Millia IslamiaDepartment of Surgical Oncology BRA-IRCH, All India Institute of Medical Sciences (AIIMS)Human Genetics Laboratory, Department of Biosciences, Jamia Millia IslamiaAbstract Globally, breast cancer is the most common cancer to affect women. There are different molecular and pathological factors that are involved in the uncontrolled proliferation of breast cancer cells. FOXN3 that is member of Forkhead box family proteins is well recognized for having a crucial role in different biological processes and is reported to be dysregulated in various malignancies. The studies to evaluate the significance of the FOXN3 gene in progression of breast cancer are still under progress. We in the current study aim to examine the FOXN3 gene expression in Indian breast cancer patients and find its clinical relevance. FOXN3 expression analysis using RT-PCR, immunohistochemistry, and western blotting was performed in tumor and normal tissue collected from 142 sporadic breast cancer patients. To identify the genetic aberrations in FOXN3 gene automated DNA sequencing was done. FOXN3 expression study revealed the elevated expression of FOXN3 mRNA in 61.26% of the cases whereas FOXN3 protein was seen to be overexpressed in 59.15% cases. Further, it was found that the elevated expression of FOXN3 mRNA was significantly correlated with the post-menopausal (p = 0.003) status and positive lymph node status (p = 0.049) of the patients. The FOXN3 protein expression also exhibited the significant association with menopausal status (p = 0.008), lymph node status (p = 0.001) and clinical stage (p = 0.018) of the patients. However, we did not find any mutation in the DNA binding domain of the FOXN3 gene in the Indian breast cancer cases. Our findings indicates that overexpression of FOXN3 gene in Indian breast cancer cases can have a potential role in breast cancer progression especially in advanced clinical stages.https://doi.org/10.1038/s41598-025-98090-0Breast cancerFOXN3mRNAExpression |
| spellingShingle | Sheersh Massey Mohammad Aasif Khan Safia Obaidur Rab Syeda Maryam Husain Asifa Khan Sadaf Zoya Mallik Saad Mustafa Rahul Kumar Maria Habib S. V. S. Deo Syed Akhtar Husain Clinical significance of FOXN3 expression in Indian breast cancer patients Scientific Reports Breast cancer FOXN3 mRNA Expression |
| title | Clinical significance of FOXN3 expression in Indian breast cancer patients |
| title_full | Clinical significance of FOXN3 expression in Indian breast cancer patients |
| title_fullStr | Clinical significance of FOXN3 expression in Indian breast cancer patients |
| title_full_unstemmed | Clinical significance of FOXN3 expression in Indian breast cancer patients |
| title_short | Clinical significance of FOXN3 expression in Indian breast cancer patients |
| title_sort | clinical significance of foxn3 expression in indian breast cancer patients |
| topic | Breast cancer FOXN3 mRNA Expression |
| url | https://doi.org/10.1038/s41598-025-98090-0 |
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