A cyanobacterial sigma factor F controls biofilm-promoting genes through intra- and intercellular pathways
Cyanobacteria frequently constitute integral components of microbial communities known as phototrophic biofilms, which are widespread in various environments. Moreover, assemblages of these organisms, which serve as an expression platform, simplify harvesting the biomass, thereby holding significant...
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| Format: | Article |
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Elsevier
2024-12-01
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| Series: | Biofilm |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S259020752400042X |
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| author | Shiran Suban Sapir Yemini Anna Shor Hiba Waldman Ben-Asher Orly Yaron Sarit Karako-Lampert Eleonora Sendersky Susan S. Golden Rakefet Schwarz |
| author_facet | Shiran Suban Sapir Yemini Anna Shor Hiba Waldman Ben-Asher Orly Yaron Sarit Karako-Lampert Eleonora Sendersky Susan S. Golden Rakefet Schwarz |
| author_sort | Shiran Suban |
| collection | DOAJ |
| description | Cyanobacteria frequently constitute integral components of microbial communities known as phototrophic biofilms, which are widespread in various environments. Moreover, assemblages of these organisms, which serve as an expression platform, simplify harvesting the biomass, thereby holding significant industrial relevance. Previous studies of the model cyanobacterium Synechococcus elongatus PCC 7942 revealed that its planktonic growth habit results from a biofilm-suppression mechanism that depends on an extracellular inhibitor, an observation that opens the door to investigating cyanobacterial intercellular communication. Here, we demonstrate that the RNA polymerase sigma factor SigF1, is required for this biofilm-suppression mechanism whereas the S. elongatus paralog SigF2 is not involved in biofilm regulation. Comprehensive transcriptome analyses identified distinct regulons under the control of each of these sigma factors. sigF1 inactivation substantially lowers transcription of genes that code for the primary pilus subunit and consequently prevents pilus assembly. Moreover, additional data demonstrate absence of the biofilm inhibitor from conditioned medium of the sigF1 mutant, further validating involvement of the pilus assembly complex in secretion of the biofilm inhibitor. Consequently, expression is significantly upregulated for the ebfG-operon that encodes matrix components and the genes that encode the corresponding secretion system, which are repressed by the biofilm inhibitor in the wild type. Thus, this study uncovers a basic regulatory component of cyanobacterial intercellular communication, a field that is in its infancy. Elevated expression of biofilm-promoting genes in a sigF1 mutant supports an additional layer of regulation by SigF1 that operates via an intracellular mechanism. |
| format | Article |
| id | doaj-art-e73aad4fd80149e1b477673ce2ad9aac |
| institution | DOAJ |
| issn | 2590-2075 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj-art-e73aad4fd80149e1b477673ce2ad9aac2025-08-20T02:50:27ZengElsevierBiofilm2590-20752024-12-01810021710.1016/j.bioflm.2024.100217A cyanobacterial sigma factor F controls biofilm-promoting genes through intra- and intercellular pathwaysShiran Suban0Sapir Yemini1Anna Shor2Hiba Waldman Ben-Asher3Orly Yaron4Sarit Karako-Lampert5Eleonora Sendersky6Susan S. Golden7Rakefet Schwarz8The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, IsraelThe Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, IsraelThe Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, IsraelThe Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, IsraelThe Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, IsraelThe Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, IsraelThe Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, IsraelDivision of Biological Sciences, University of California, San Diego, La Jolla, CA, 92093, USA; Center for Circadian Biology, University of California, San Diego, La Jolla, CA, 92093, USAThe Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, Israel; Corresponding author.Cyanobacteria frequently constitute integral components of microbial communities known as phototrophic biofilms, which are widespread in various environments. Moreover, assemblages of these organisms, which serve as an expression platform, simplify harvesting the biomass, thereby holding significant industrial relevance. Previous studies of the model cyanobacterium Synechococcus elongatus PCC 7942 revealed that its planktonic growth habit results from a biofilm-suppression mechanism that depends on an extracellular inhibitor, an observation that opens the door to investigating cyanobacterial intercellular communication. Here, we demonstrate that the RNA polymerase sigma factor SigF1, is required for this biofilm-suppression mechanism whereas the S. elongatus paralog SigF2 is not involved in biofilm regulation. Comprehensive transcriptome analyses identified distinct regulons under the control of each of these sigma factors. sigF1 inactivation substantially lowers transcription of genes that code for the primary pilus subunit and consequently prevents pilus assembly. Moreover, additional data demonstrate absence of the biofilm inhibitor from conditioned medium of the sigF1 mutant, further validating involvement of the pilus assembly complex in secretion of the biofilm inhibitor. Consequently, expression is significantly upregulated for the ebfG-operon that encodes matrix components and the genes that encode the corresponding secretion system, which are repressed by the biofilm inhibitor in the wild type. Thus, this study uncovers a basic regulatory component of cyanobacterial intercellular communication, a field that is in its infancy. Elevated expression of biofilm-promoting genes in a sigF1 mutant supports an additional layer of regulation by SigF1 that operates via an intracellular mechanism.http://www.sciencedirect.com/science/article/pii/S259020752400042X |
| spellingShingle | Shiran Suban Sapir Yemini Anna Shor Hiba Waldman Ben-Asher Orly Yaron Sarit Karako-Lampert Eleonora Sendersky Susan S. Golden Rakefet Schwarz A cyanobacterial sigma factor F controls biofilm-promoting genes through intra- and intercellular pathways Biofilm |
| title | A cyanobacterial sigma factor F controls biofilm-promoting genes through intra- and intercellular pathways |
| title_full | A cyanobacterial sigma factor F controls biofilm-promoting genes through intra- and intercellular pathways |
| title_fullStr | A cyanobacterial sigma factor F controls biofilm-promoting genes through intra- and intercellular pathways |
| title_full_unstemmed | A cyanobacterial sigma factor F controls biofilm-promoting genes through intra- and intercellular pathways |
| title_short | A cyanobacterial sigma factor F controls biofilm-promoting genes through intra- and intercellular pathways |
| title_sort | cyanobacterial sigma factor f controls biofilm promoting genes through intra and intercellular pathways |
| url | http://www.sciencedirect.com/science/article/pii/S259020752400042X |
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