Acupoint Injection of Autologous Stromal Vascular Fraction and Allogeneic Adipose-Derived Stem Cells to Treat Hip Dysplasia in Dogs

Stem cells isolated from adipose tissue show great therapeutic potential in veterinary medicine, but some points such as the use of fresh or cultured cells and route of administration need better knowledge. This study aimed to evaluate the effect of autologous stromal vascular fraction (SVF, n=4) or...

Full description

Saved in:
Bibliographic Details
Main Authors: Camila Marx, Maiele Dornelles Silveira, Isabel Selbach, Ariel Silveira da Silva, Luisa Maria Gomes de Macedo Braga, Melissa Camassola, Nance Beyer Nardi
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.1155/2014/391274
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849467478465314816
author Camila Marx
Maiele Dornelles Silveira
Isabel Selbach
Ariel Silveira da Silva
Luisa Maria Gomes de Macedo Braga
Melissa Camassola
Nance Beyer Nardi
author_facet Camila Marx
Maiele Dornelles Silveira
Isabel Selbach
Ariel Silveira da Silva
Luisa Maria Gomes de Macedo Braga
Melissa Camassola
Nance Beyer Nardi
author_sort Camila Marx
collection DOAJ
description Stem cells isolated from adipose tissue show great therapeutic potential in veterinary medicine, but some points such as the use of fresh or cultured cells and route of administration need better knowledge. This study aimed to evaluate the effect of autologous stromal vascular fraction (SVF, n=4) or allogeneic cultured adipose-derived stem cells (ASCs, n=5) injected into acupuncture points in dogs with hip dysplasia and weak response to drug therapy. Canine ASCs have proliferation and differentiation potential similar to ASCs from other species. After the first week of treatment, clinical evaluation showed marked improvement compared with baseline results in all patients treated with autologous SVF and three of the dogs treated with allogeneic ASCs. On days 15 and 30, all dogs showed improvement in range of motion, lameness at trot, and pain on manipulation of the joints, except for one ASC-treated patient. Positive results were more clearly seen in the SVF-treated group. These results show that autologous SVF or allogeneic ASCs can be safely used in acupoint injection for treating hip dysplasia in dogs and represent an important therapeutic alternative for this type of pathology. Further studies are necessary to assess a possible advantage of SVF cells in treating joint diseases.
format Article
id doaj-art-e72d1d4c8b4f41bc9ea097868f2ede2f
institution Kabale University
issn 1687-966X
1687-9678
language English
publishDate 2014-01-01
publisher Wiley
record_format Article
series Stem Cells International
spelling doaj-art-e72d1d4c8b4f41bc9ea097868f2ede2f2025-08-20T03:26:11ZengWileyStem Cells International1687-966X1687-96782014-01-01201410.1155/2014/391274391274Acupoint Injection of Autologous Stromal Vascular Fraction and Allogeneic Adipose-Derived Stem Cells to Treat Hip Dysplasia in DogsCamila Marx0Maiele Dornelles Silveira1Isabel Selbach2Ariel Silveira da Silva3Luisa Maria Gomes de Macedo Braga4Melissa Camassola5Nance Beyer Nardi6Laboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, 92425-900 Canoas, RS, BrazilLaboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, 92425-900 Canoas, RS, BrazilClinivetis Clinica Veterinaria, 93310-500 Novo Hamburgo, RS, BrazilLaboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, 92425-900 Canoas, RS, BrazilCenter for Experimental Biological Models, Pontificia Universidade Catolica do Rio Grande do Sul, 90619-900 Porto Alegre, RS, BrazilLaboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, 92425-900 Canoas, RS, BrazilLaboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, 92425-900 Canoas, RS, BrazilStem cells isolated from adipose tissue show great therapeutic potential in veterinary medicine, but some points such as the use of fresh or cultured cells and route of administration need better knowledge. This study aimed to evaluate the effect of autologous stromal vascular fraction (SVF, n=4) or allogeneic cultured adipose-derived stem cells (ASCs, n=5) injected into acupuncture points in dogs with hip dysplasia and weak response to drug therapy. Canine ASCs have proliferation and differentiation potential similar to ASCs from other species. After the first week of treatment, clinical evaluation showed marked improvement compared with baseline results in all patients treated with autologous SVF and three of the dogs treated with allogeneic ASCs. On days 15 and 30, all dogs showed improvement in range of motion, lameness at trot, and pain on manipulation of the joints, except for one ASC-treated patient. Positive results were more clearly seen in the SVF-treated group. These results show that autologous SVF or allogeneic ASCs can be safely used in acupoint injection for treating hip dysplasia in dogs and represent an important therapeutic alternative for this type of pathology. Further studies are necessary to assess a possible advantage of SVF cells in treating joint diseases.http://dx.doi.org/10.1155/2014/391274
spellingShingle Camila Marx
Maiele Dornelles Silveira
Isabel Selbach
Ariel Silveira da Silva
Luisa Maria Gomes de Macedo Braga
Melissa Camassola
Nance Beyer Nardi
Acupoint Injection of Autologous Stromal Vascular Fraction and Allogeneic Adipose-Derived Stem Cells to Treat Hip Dysplasia in Dogs
Stem Cells International
title Acupoint Injection of Autologous Stromal Vascular Fraction and Allogeneic Adipose-Derived Stem Cells to Treat Hip Dysplasia in Dogs
title_full Acupoint Injection of Autologous Stromal Vascular Fraction and Allogeneic Adipose-Derived Stem Cells to Treat Hip Dysplasia in Dogs
title_fullStr Acupoint Injection of Autologous Stromal Vascular Fraction and Allogeneic Adipose-Derived Stem Cells to Treat Hip Dysplasia in Dogs
title_full_unstemmed Acupoint Injection of Autologous Stromal Vascular Fraction and Allogeneic Adipose-Derived Stem Cells to Treat Hip Dysplasia in Dogs
title_short Acupoint Injection of Autologous Stromal Vascular Fraction and Allogeneic Adipose-Derived Stem Cells to Treat Hip Dysplasia in Dogs
title_sort acupoint injection of autologous stromal vascular fraction and allogeneic adipose derived stem cells to treat hip dysplasia in dogs
url http://dx.doi.org/10.1155/2014/391274
work_keys_str_mv AT camilamarx acupointinjectionofautologousstromalvascularfractionandallogeneicadiposederivedstemcellstotreathipdysplasiaindogs
AT maieledornellessilveira acupointinjectionofautologousstromalvascularfractionandallogeneicadiposederivedstemcellstotreathipdysplasiaindogs
AT isabelselbach acupointinjectionofautologousstromalvascularfractionandallogeneicadiposederivedstemcellstotreathipdysplasiaindogs
AT arielsilveiradasilva acupointinjectionofautologousstromalvascularfractionandallogeneicadiposederivedstemcellstotreathipdysplasiaindogs
AT luisamariagomesdemacedobraga acupointinjectionofautologousstromalvascularfractionandallogeneicadiposederivedstemcellstotreathipdysplasiaindogs
AT melissacamassola acupointinjectionofautologousstromalvascularfractionandallogeneicadiposederivedstemcellstotreathipdysplasiaindogs
AT nancebeyernardi acupointinjectionofautologousstromalvascularfractionandallogeneicadiposederivedstemcellstotreathipdysplasiaindogs