The role of learned song in the evolution and speciation of Eastern and Spotted towhees.

Oscine songbirds learn vocalizations that function in mate attraction and territory defense; sexual selection pressures on these learned songs could thus accelerate speciation. The Eastern and Spotted towhees are recently diverged sister species that now have partially overlapping ranges with eviden...

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Main Authors: Ximena León Du'Mottuchi, Nicole Creanza
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-06-01
Series:PLoS Computational Biology
Online Access:https://doi.org/10.1371/journal.pcbi.1013135
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author Ximena León Du'Mottuchi
Nicole Creanza
author_facet Ximena León Du'Mottuchi
Nicole Creanza
author_sort Ximena León Du'Mottuchi
collection DOAJ
description Oscine songbirds learn vocalizations that function in mate attraction and territory defense; sexual selection pressures on these learned songs could thus accelerate speciation. The Eastern and Spotted towhees are recently diverged sister species that now have partially overlapping ranges with evidence of some hybridization. Widespread community-science recordings of these species, including songs within their zone of overlap and from potential hybrids, enable us to investigate whether song differentiation might facilitate their reproductive isolation. Here, we quantify 16 song features to analyze geographic variation in Spotted and Eastern towhee songs and assess species-level differences. We then use several machine learning models to measure how accurately their songs can be classified by species. While no single song feature reliably distinguishes the two species, machine learning models classified songs with relatively high accuracy (random forest: 89.5%, deep learning: 90%, gradient boosting machine: 88%, convolutional neural network: 88%); interestingly, species classification was less accurate in their zone of overlap. Finally, our analysis of the limited publicly available genetic data from each species supports the hypothesis that the species are reproductively isolated. Together, our results suggest that small variations in multiple features may contribute to these sister species' ability to recognize their species-specific songs.
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spelling doaj-art-e72bc1b87e63460f931edb3cc7ffed932025-08-20T03:28:54ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582025-06-01216e101313510.1371/journal.pcbi.1013135The role of learned song in the evolution and speciation of Eastern and Spotted towhees.Ximena León Du'MottuchiNicole CreanzaOscine songbirds learn vocalizations that function in mate attraction and territory defense; sexual selection pressures on these learned songs could thus accelerate speciation. The Eastern and Spotted towhees are recently diverged sister species that now have partially overlapping ranges with evidence of some hybridization. Widespread community-science recordings of these species, including songs within their zone of overlap and from potential hybrids, enable us to investigate whether song differentiation might facilitate their reproductive isolation. Here, we quantify 16 song features to analyze geographic variation in Spotted and Eastern towhee songs and assess species-level differences. We then use several machine learning models to measure how accurately their songs can be classified by species. While no single song feature reliably distinguishes the two species, machine learning models classified songs with relatively high accuracy (random forest: 89.5%, deep learning: 90%, gradient boosting machine: 88%, convolutional neural network: 88%); interestingly, species classification was less accurate in their zone of overlap. Finally, our analysis of the limited publicly available genetic data from each species supports the hypothesis that the species are reproductively isolated. Together, our results suggest that small variations in multiple features may contribute to these sister species' ability to recognize their species-specific songs.https://doi.org/10.1371/journal.pcbi.1013135
spellingShingle Ximena León Du'Mottuchi
Nicole Creanza
The role of learned song in the evolution and speciation of Eastern and Spotted towhees.
PLoS Computational Biology
title The role of learned song in the evolution and speciation of Eastern and Spotted towhees.
title_full The role of learned song in the evolution and speciation of Eastern and Spotted towhees.
title_fullStr The role of learned song in the evolution and speciation of Eastern and Spotted towhees.
title_full_unstemmed The role of learned song in the evolution and speciation of Eastern and Spotted towhees.
title_short The role of learned song in the evolution and speciation of Eastern and Spotted towhees.
title_sort role of learned song in the evolution and speciation of eastern and spotted towhees
url https://doi.org/10.1371/journal.pcbi.1013135
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