Machine Learning-Driven Prediction of CRISPR-Cas9 Off-Target Effects and Mechanistic Insights
The precise prediction of off-target effects in CRISPR-Cas9 genome editing is critical for ensuring the safety and efficacy of this powerful tool. This study leverages machine learning techniques to predict off-target cleavage sites and investigate the underlying mechanisms that affect cleavage effi...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2024-10-01
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| Series: | The EuroBiotech Journal |
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| Online Access: | https://doi.org/10.2478/ebtj-2024-0020 |
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| _version_ | 1850194658036023296 |
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| author | Bhardwaj Anuradha Tomar Pradeep Nain Vikrant |
| author_facet | Bhardwaj Anuradha Tomar Pradeep Nain Vikrant |
| author_sort | Bhardwaj Anuradha |
| collection | DOAJ |
| description | The precise prediction of off-target effects in CRISPR-Cas9 genome editing is critical for ensuring the safety and efficacy of this powerful tool. This study leverages machine learning techniques to predict off-target cleavage sites and investigate the underlying mechanisms that affect cleavage efficiencies. By integrating data from Tsai et al. and Kleinsteiver et al., who employed the GUIDE-seq method, we aim to enhance our understanding of the factors influencing CRISPR-Cas9 activity. |
| format | Article |
| id | doaj-art-e723cd04e3354f1f92a28023e9e5ba80 |
| institution | OA Journals |
| issn | 2564-615X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Sciendo |
| record_format | Article |
| series | The EuroBiotech Journal |
| spelling | doaj-art-e723cd04e3354f1f92a28023e9e5ba802025-08-20T02:13:56ZengSciendoThe EuroBiotech Journal2564-615X2024-10-018421322910.2478/ebtj-2024-0020Machine Learning-Driven Prediction of CRISPR-Cas9 Off-Target Effects and Mechanistic InsightsBhardwaj Anuradha0Tomar Pradeep1Nain Vikrant2School of Biotechnology, Gautam Buddha University, Greater Noida, Uttar Pradesh, 201312, IndiaSchool of Information and Communication Technology, Gautam Buddha University, Greater Noida, Uttar Pradesh, 201312, IndiaSchool of Biotechnology, Gautam Buddha University, Greater Noida, Uttar Pradesh, 201312, IndiaThe precise prediction of off-target effects in CRISPR-Cas9 genome editing is critical for ensuring the safety and efficacy of this powerful tool. This study leverages machine learning techniques to predict off-target cleavage sites and investigate the underlying mechanisms that affect cleavage efficiencies. By integrating data from Tsai et al. and Kleinsteiver et al., who employed the GUIDE-seq method, we aim to enhance our understanding of the factors influencing CRISPR-Cas9 activity.https://doi.org/10.2478/ebtj-2024-0020crispr-cas9machine learningon-targetsoff-targetsgenome editing |
| spellingShingle | Bhardwaj Anuradha Tomar Pradeep Nain Vikrant Machine Learning-Driven Prediction of CRISPR-Cas9 Off-Target Effects and Mechanistic Insights The EuroBiotech Journal crispr-cas9 machine learning on-targets off-targets genome editing |
| title | Machine Learning-Driven Prediction of CRISPR-Cas9 Off-Target Effects and Mechanistic Insights |
| title_full | Machine Learning-Driven Prediction of CRISPR-Cas9 Off-Target Effects and Mechanistic Insights |
| title_fullStr | Machine Learning-Driven Prediction of CRISPR-Cas9 Off-Target Effects and Mechanistic Insights |
| title_full_unstemmed | Machine Learning-Driven Prediction of CRISPR-Cas9 Off-Target Effects and Mechanistic Insights |
| title_short | Machine Learning-Driven Prediction of CRISPR-Cas9 Off-Target Effects and Mechanistic Insights |
| title_sort | machine learning driven prediction of crispr cas9 off target effects and mechanistic insights |
| topic | crispr-cas9 machine learning on-targets off-targets genome editing |
| url | https://doi.org/10.2478/ebtj-2024-0020 |
| work_keys_str_mv | AT bhardwajanuradha machinelearningdrivenpredictionofcrisprcas9offtargeteffectsandmechanisticinsights AT tomarpradeep machinelearningdrivenpredictionofcrisprcas9offtargeteffectsandmechanisticinsights AT nainvikrant machinelearningdrivenpredictionofcrisprcas9offtargeteffectsandmechanisticinsights |