Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory module.

Plant cell growth involves coordination of numerous processes and signaling cascades among the different cellular compartments to concomitantly enlarge the protoplast and the surrounding cell wall. The cell wall integrity-sensing process involves the extracellular LRX (LRR-Extensin) proteins that bi...

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Main Authors: Shibu Gupta, Amandine Guérin, Aline Herger, Xiaoyu Hou, Myriam Schaufelberger, Romain Roulard, Anouck Diet, Stefan Roffler, Valérie Lefebvre, Thomas Wicker, Jérôme Pelloux, Christoph Ringli
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2024-01-01
Series:PLoS Genetics
Online Access:https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1011087&type=printable
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author Shibu Gupta
Amandine Guérin
Aline Herger
Xiaoyu Hou
Myriam Schaufelberger
Romain Roulard
Anouck Diet
Stefan Roffler
Valérie Lefebvre
Thomas Wicker
Jérôme Pelloux
Christoph Ringli
author_facet Shibu Gupta
Amandine Guérin
Aline Herger
Xiaoyu Hou
Myriam Schaufelberger
Romain Roulard
Anouck Diet
Stefan Roffler
Valérie Lefebvre
Thomas Wicker
Jérôme Pelloux
Christoph Ringli
author_sort Shibu Gupta
collection DOAJ
description Plant cell growth involves coordination of numerous processes and signaling cascades among the different cellular compartments to concomitantly enlarge the protoplast and the surrounding cell wall. The cell wall integrity-sensing process involves the extracellular LRX (LRR-Extensin) proteins that bind RALF (Rapid ALkalinization Factor) peptide hormones and, in vegetative tissues, interact with the transmembrane receptor kinase FERONIA (FER). This LRX/RALF/FER signaling module influences cell wall composition and regulates cell growth. The numerous proteins involved in or influenced by this module are beginning to be characterized. In a genetic screen, mutations in Apyrase 7 (APY7) were identified to suppress growth defects observed in lrx1 and fer mutants. APY7 encodes a Golgi-localized NTP-diphosphohydrolase, but opposed to other apyrases of Arabidopsis, APY7 revealed to be a negative regulator of cell growth. APY7 modulates the growth-inhibiting effect of RALF1, influences the cell wall architecture and -composition, and alters the pH of the extracellular matrix, all of which affect cell growth. Together, this study reveals a function of APY7 in cell wall formation and cell growth that is connected to growth processes influenced by the LRX/RALF/FER signaling module.
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issn 1553-7390
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language English
publishDate 2024-01-01
publisher Public Library of Science (PLoS)
record_format Article
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spelling doaj-art-e721381661134373b4b30c127d963e4d2025-08-20T02:11:38ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042024-01-01201e101108710.1371/journal.pgen.1011087Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory module.Shibu GuptaAmandine GuérinAline HergerXiaoyu HouMyriam SchaufelbergerRomain RoulardAnouck DietStefan RofflerValérie LefebvreThomas WickerJérôme PellouxChristoph RingliPlant cell growth involves coordination of numerous processes and signaling cascades among the different cellular compartments to concomitantly enlarge the protoplast and the surrounding cell wall. The cell wall integrity-sensing process involves the extracellular LRX (LRR-Extensin) proteins that bind RALF (Rapid ALkalinization Factor) peptide hormones and, in vegetative tissues, interact with the transmembrane receptor kinase FERONIA (FER). This LRX/RALF/FER signaling module influences cell wall composition and regulates cell growth. The numerous proteins involved in or influenced by this module are beginning to be characterized. In a genetic screen, mutations in Apyrase 7 (APY7) were identified to suppress growth defects observed in lrx1 and fer mutants. APY7 encodes a Golgi-localized NTP-diphosphohydrolase, but opposed to other apyrases of Arabidopsis, APY7 revealed to be a negative regulator of cell growth. APY7 modulates the growth-inhibiting effect of RALF1, influences the cell wall architecture and -composition, and alters the pH of the extracellular matrix, all of which affect cell growth. Together, this study reveals a function of APY7 in cell wall formation and cell growth that is connected to growth processes influenced by the LRX/RALF/FER signaling module.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1011087&type=printable
spellingShingle Shibu Gupta
Amandine Guérin
Aline Herger
Xiaoyu Hou
Myriam Schaufelberger
Romain Roulard
Anouck Diet
Stefan Roffler
Valérie Lefebvre
Thomas Wicker
Jérôme Pelloux
Christoph Ringli
Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory module.
PLoS Genetics
title Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory module.
title_full Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory module.
title_fullStr Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory module.
title_full_unstemmed Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory module.
title_short Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory module.
title_sort growth inhibiting effects of the unconventional plant apyrase 7 of arabidopsis thaliana influences the lrx ralf fer growth regulatory module
url https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1011087&type=printable
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