Murine Nephrotoxic Nephritis as a Model of Chronic Kidney Disease
Using the nonaccelerated murine nephrotoxic nephritis (NTN) as a model of chronic kidney disease (CKD) could provide an easily inducible model that enables a rapid test of treatments. Originally, the NTN model was developed as an acute model of glomerulonephritis, but in this study we evaluate the m...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Nephrology |
| Online Access: | http://dx.doi.org/10.1155/2018/8424502 |
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| author | M. K. E. Ougaard P. H. Kvist H. E. Jensen C. Hess I. Rune H. Søndergaard |
| author_facet | M. K. E. Ougaard P. H. Kvist H. E. Jensen C. Hess I. Rune H. Søndergaard |
| author_sort | M. K. E. Ougaard |
| collection | DOAJ |
| description | Using the nonaccelerated murine nephrotoxic nephritis (NTN) as a model of chronic kidney disease (CKD) could provide an easily inducible model that enables a rapid test of treatments. Originally, the NTN model was developed as an acute model of glomerulonephritis, but in this study we evaluate the model as a CKD model and compare CD1 and C57BL/6 female and male mice. CD1 mice have previously showed an increased susceptibility to CKD in other CKD models. NTN was induced by injecting nephrotoxic serum (NTS) and evaluated by CKD parameters including albuminuria, glomerular filtration rate (GFR), mesangial expansion, and renal fibrosis. Both strains showed significant albuminuria on days 2-3 which remained significant until the last time point on days 36-37 supporting dysfunctional filtration also observed by a significantly declined GFR on days 5-6, 15–17, and 34–37. Both strains showed early progressive mesangial expansion and significant renal fibrosis within three weeks suggesting CKD development. CD1 and C57BL/6 females showed a similar disease progression, but female mice seemed more susceptible to NTS compared to male mice. The presence of albuminuria, GFR decline, mesangial expansion, and fibrosis showed that the NTN model is a relevant CKD model both in C57BL/6 and in CD1 mice. |
| format | Article |
| id | doaj-art-e71daae3131e4b92aa47568fc8f56de9 |
| institution | Kabale University |
| issn | 2090-214X 2090-2158 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Nephrology |
| spelling | doaj-art-e71daae3131e4b92aa47568fc8f56de92025-08-20T03:26:10ZengWileyInternational Journal of Nephrology2090-214X2090-21582018-01-01201810.1155/2018/84245028424502Murine Nephrotoxic Nephritis as a Model of Chronic Kidney DiseaseM. K. E. Ougaard0P. H. Kvist1H. E. Jensen2C. Hess3I. Rune4H. Søndergaard5Department of Diabetes Complications Pharmacology, Novo Nordisk, Maaloev, DenmarkDepartment of Histology and Bioimaging, Novo Nordisk, Maaloev, DenmarkDepartment of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, DenmarkDepartment of Diabetes Complications Pharmacology, Novo Nordisk, Maaloev, DenmarkDepartment of Diabetes Complications Pharmacology, Novo Nordisk, Maaloev, DenmarkDepartment of Diabetes Complications Pharmacology, Novo Nordisk, Maaloev, DenmarkUsing the nonaccelerated murine nephrotoxic nephritis (NTN) as a model of chronic kidney disease (CKD) could provide an easily inducible model that enables a rapid test of treatments. Originally, the NTN model was developed as an acute model of glomerulonephritis, but in this study we evaluate the model as a CKD model and compare CD1 and C57BL/6 female and male mice. CD1 mice have previously showed an increased susceptibility to CKD in other CKD models. NTN was induced by injecting nephrotoxic serum (NTS) and evaluated by CKD parameters including albuminuria, glomerular filtration rate (GFR), mesangial expansion, and renal fibrosis. Both strains showed significant albuminuria on days 2-3 which remained significant until the last time point on days 36-37 supporting dysfunctional filtration also observed by a significantly declined GFR on days 5-6, 15–17, and 34–37. Both strains showed early progressive mesangial expansion and significant renal fibrosis within three weeks suggesting CKD development. CD1 and C57BL/6 females showed a similar disease progression, but female mice seemed more susceptible to NTS compared to male mice. The presence of albuminuria, GFR decline, mesangial expansion, and fibrosis showed that the NTN model is a relevant CKD model both in C57BL/6 and in CD1 mice.http://dx.doi.org/10.1155/2018/8424502 |
| spellingShingle | M. K. E. Ougaard P. H. Kvist H. E. Jensen C. Hess I. Rune H. Søndergaard Murine Nephrotoxic Nephritis as a Model of Chronic Kidney Disease International Journal of Nephrology |
| title | Murine Nephrotoxic Nephritis as a Model of Chronic Kidney Disease |
| title_full | Murine Nephrotoxic Nephritis as a Model of Chronic Kidney Disease |
| title_fullStr | Murine Nephrotoxic Nephritis as a Model of Chronic Kidney Disease |
| title_full_unstemmed | Murine Nephrotoxic Nephritis as a Model of Chronic Kidney Disease |
| title_short | Murine Nephrotoxic Nephritis as a Model of Chronic Kidney Disease |
| title_sort | murine nephrotoxic nephritis as a model of chronic kidney disease |
| url | http://dx.doi.org/10.1155/2018/8424502 |
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