Mogrosides Protect Against Diabetic Kidney Injury via Inhibiting Macrophage Activation in a Mouse Model

Diabetic kidney injury is an almost unavoidable complication in diabetic patients. The activation of macrophages with high glucose in the patient’s body is a key factor in triggering diabetic kidney disease (DKD). Mogrosides are commonly used sweeteners, but their effects on diabetic kidney injury a...

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Main Authors: Fangyi Jiang, Xiaoli Huang, Man Yan, Jiajun Tan, Xueyun Dong, Xianhai Liu, Jiayuan He, Asmaa Ali, Min Chen, Leilei Zhang, Liang Wu, Pingping Wang
Format: Article
Language:English
Published: Wiley 2025-01-01
Series:Journal of Diabetes Research
Online Access:http://dx.doi.org/10.1155/jdr/5291562
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author Fangyi Jiang
Xiaoli Huang
Man Yan
Jiajun Tan
Xueyun Dong
Xianhai Liu
Jiayuan He
Asmaa Ali
Min Chen
Leilei Zhang
Liang Wu
Pingping Wang
author_facet Fangyi Jiang
Xiaoli Huang
Man Yan
Jiajun Tan
Xueyun Dong
Xianhai Liu
Jiayuan He
Asmaa Ali
Min Chen
Leilei Zhang
Liang Wu
Pingping Wang
author_sort Fangyi Jiang
collection DOAJ
description Diabetic kidney injury is an almost unavoidable complication in diabetic patients. The activation of macrophages with high glucose in the patient’s body is a key factor in triggering diabetic kidney disease (DKD). Mogrosides are commonly used sweeteners, but their effects on diabetic kidney injury are still unclear. This study used THP-1 cell models and diabetic mouse models to examine the impacts and pathways of mogrosides in inhibiting hyperglycemia-activated macrophages and alleviating kidney damage. This study used high glucose (33.3 mmol/L) to induce activation of macrophage-like THP-1 cells for studying the anti-inflammatory mechanism of mogrosides. At the same time, a diabetic mouse model was prepared using a high-fat diet and intraperitoneal injection of streptozotocin in order to further study the effects of mogrosides on alleviating symptoms of DKD. Mogrosides can suppress the activation of macrophages and kidney damage in diabetic mice, and this anti-inflammatory effect seems to be mediated through the NF-κB/NLRP3/Caspase-1 axis in macrophages. Moreover, the metabolomic results revealed that the anti-inflammatory properties of mogrosides were associated with the modulation of glutamate metabolism and glycerophospholipid metabolism in macrophages. Our results indicated that supplementing diabetic patients with mogrosides may help inhibit inflammatory responses and prevent kidney damage.
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spelling doaj-art-e717f5b1af0e4601ab94b82d4317fe242025-08-20T03:15:51ZengWileyJournal of Diabetes Research2314-67532025-01-01202510.1155/jdr/5291562Mogrosides Protect Against Diabetic Kidney Injury via Inhibiting Macrophage Activation in a Mouse ModelFangyi Jiang0Xiaoli Huang1Man Yan2Jiajun Tan3Xueyun Dong4Xianhai Liu5Jiayuan He6Asmaa Ali7Min Chen8Leilei Zhang9Liang Wu10Pingping Wang11Department of Laboratory MedicineDepartment of Laboratory MedicineZhenjiang City Central Blood StationDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineHealth Testing CenterDepartment of Laboratory MedicinePublic Experiment and Service CenterDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineDiabetic kidney injury is an almost unavoidable complication in diabetic patients. The activation of macrophages with high glucose in the patient’s body is a key factor in triggering diabetic kidney disease (DKD). Mogrosides are commonly used sweeteners, but their effects on diabetic kidney injury are still unclear. This study used THP-1 cell models and diabetic mouse models to examine the impacts and pathways of mogrosides in inhibiting hyperglycemia-activated macrophages and alleviating kidney damage. This study used high glucose (33.3 mmol/L) to induce activation of macrophage-like THP-1 cells for studying the anti-inflammatory mechanism of mogrosides. At the same time, a diabetic mouse model was prepared using a high-fat diet and intraperitoneal injection of streptozotocin in order to further study the effects of mogrosides on alleviating symptoms of DKD. Mogrosides can suppress the activation of macrophages and kidney damage in diabetic mice, and this anti-inflammatory effect seems to be mediated through the NF-κB/NLRP3/Caspase-1 axis in macrophages. Moreover, the metabolomic results revealed that the anti-inflammatory properties of mogrosides were associated with the modulation of glutamate metabolism and glycerophospholipid metabolism in macrophages. Our results indicated that supplementing diabetic patients with mogrosides may help inhibit inflammatory responses and prevent kidney damage.http://dx.doi.org/10.1155/jdr/5291562
spellingShingle Fangyi Jiang
Xiaoli Huang
Man Yan
Jiajun Tan
Xueyun Dong
Xianhai Liu
Jiayuan He
Asmaa Ali
Min Chen
Leilei Zhang
Liang Wu
Pingping Wang
Mogrosides Protect Against Diabetic Kidney Injury via Inhibiting Macrophage Activation in a Mouse Model
Journal of Diabetes Research
title Mogrosides Protect Against Diabetic Kidney Injury via Inhibiting Macrophage Activation in a Mouse Model
title_full Mogrosides Protect Against Diabetic Kidney Injury via Inhibiting Macrophage Activation in a Mouse Model
title_fullStr Mogrosides Protect Against Diabetic Kidney Injury via Inhibiting Macrophage Activation in a Mouse Model
title_full_unstemmed Mogrosides Protect Against Diabetic Kidney Injury via Inhibiting Macrophage Activation in a Mouse Model
title_short Mogrosides Protect Against Diabetic Kidney Injury via Inhibiting Macrophage Activation in a Mouse Model
title_sort mogrosides protect against diabetic kidney injury via inhibiting macrophage activation in a mouse model
url http://dx.doi.org/10.1155/jdr/5291562
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