Biofabricating Three-Dimensional Bacterial Cellulose Composites Using Waste-Derived Scaffolds

Microorganisms metabolising low-value carbon sources can produce a diverse range of bio-based and biodegradable materials compatible with circular economy principles. One such material is bacterial cellulose (BC), which can be obtained in high purity through the fermentation of sweetened tea by a Sy...

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Main Authors: Jula Kniep, Manu Thundathil, Kurosch Rezwan, Ali Reza Nazmi
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Applied Sciences
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Online Access:https://www.mdpi.com/2076-3417/15/12/6396
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author Jula Kniep
Manu Thundathil
Kurosch Rezwan
Ali Reza Nazmi
author_facet Jula Kniep
Manu Thundathil
Kurosch Rezwan
Ali Reza Nazmi
author_sort Jula Kniep
collection DOAJ
description Microorganisms metabolising low-value carbon sources can produce a diverse range of bio-based and biodegradable materials compatible with circular economy principles. One such material is bacterial cellulose (BC), which can be obtained in high purity through the fermentation of sweetened tea by a Symbiotic Culture of Bacteria and Yeast (SCOBY). In recent years, there has been a growing research interest in SCOBYs as a promising solution for sustainable material design. In this work, we have explored a novel method to grow SCOBYs vertically using a waste-based scaffold system. Waste sheep wool and cotton fabric were soaked in a SCOBY infusion to serve as scaffolds, carrying the infusion and facilitating vertical growth through capillary forces. Remarkably, vertical membrane growth up to 5 cm above the liquid–air interface (LAI) was observed after just one week. Membranes with different microstructures were found in sheep wool and cotton, randomly oriented between the scaffold fibre, resulting in a high surface area. This study demonstrated that vertical growth in scaffolds is possible, proving the concept of a new method of growing composite materials with potential high-value applications in biomedicine, energy storage, or filtration.
format Article
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issn 2076-3417
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publishDate 2025-06-01
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spelling doaj-art-e71419e4e4134a048a2b239ed0eaaf322025-08-20T02:24:39ZengMDPI AGApplied Sciences2076-34172025-06-011512639610.3390/app15126396Biofabricating Three-Dimensional Bacterial Cellulose Composites Using Waste-Derived ScaffoldsJula Kniep0Manu Thundathil1Kurosch Rezwan2Ali Reza Nazmi3Advanced Ceramics, Universität Bremen, 28359 Bremen, GermanySchool of Product Design, University of Canterbury, Private Bag 4800, Christchurch 8140, New ZealandMAPEX—Center for Materials and Processes, University of Bremen, 28334 Bremen, GermanySchool of Product Design, University of Canterbury, Private Bag 4800, Christchurch 8140, New ZealandMicroorganisms metabolising low-value carbon sources can produce a diverse range of bio-based and biodegradable materials compatible with circular economy principles. One such material is bacterial cellulose (BC), which can be obtained in high purity through the fermentation of sweetened tea by a Symbiotic Culture of Bacteria and Yeast (SCOBY). In recent years, there has been a growing research interest in SCOBYs as a promising solution for sustainable material design. In this work, we have explored a novel method to grow SCOBYs vertically using a waste-based scaffold system. Waste sheep wool and cotton fabric were soaked in a SCOBY infusion to serve as scaffolds, carrying the infusion and facilitating vertical growth through capillary forces. Remarkably, vertical membrane growth up to 5 cm above the liquid–air interface (LAI) was observed after just one week. Membranes with different microstructures were found in sheep wool and cotton, randomly oriented between the scaffold fibre, resulting in a high surface area. This study demonstrated that vertical growth in scaffolds is possible, proving the concept of a new method of growing composite materials with potential high-value applications in biomedicine, energy storage, or filtration.https://www.mdpi.com/2076-3417/15/12/6396bacterial celluloseSymbiotic Culture of Bacteria and YeastSCOBYthree-dimensional growthlocal waste streamsbiomaterial scaffold
spellingShingle Jula Kniep
Manu Thundathil
Kurosch Rezwan
Ali Reza Nazmi
Biofabricating Three-Dimensional Bacterial Cellulose Composites Using Waste-Derived Scaffolds
Applied Sciences
bacterial cellulose
Symbiotic Culture of Bacteria and Yeast
SCOBY
three-dimensional growth
local waste streams
biomaterial scaffold
title Biofabricating Three-Dimensional Bacterial Cellulose Composites Using Waste-Derived Scaffolds
title_full Biofabricating Three-Dimensional Bacterial Cellulose Composites Using Waste-Derived Scaffolds
title_fullStr Biofabricating Three-Dimensional Bacterial Cellulose Composites Using Waste-Derived Scaffolds
title_full_unstemmed Biofabricating Three-Dimensional Bacterial Cellulose Composites Using Waste-Derived Scaffolds
title_short Biofabricating Three-Dimensional Bacterial Cellulose Composites Using Waste-Derived Scaffolds
title_sort biofabricating three dimensional bacterial cellulose composites using waste derived scaffolds
topic bacterial cellulose
Symbiotic Culture of Bacteria and Yeast
SCOBY
three-dimensional growth
local waste streams
biomaterial scaffold
url https://www.mdpi.com/2076-3417/15/12/6396
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AT manuthundathil biofabricatingthreedimensionalbacterialcellulosecompositesusingwastederivedscaffolds
AT kuroschrezwan biofabricatingthreedimensionalbacterialcellulosecompositesusingwastederivedscaffolds
AT alirezanazmi biofabricatingthreedimensionalbacterialcellulosecompositesusingwastederivedscaffolds