Extensively Targeted Metabolomics Analysis of Red and Yellow Wolfberries
In the pursuit of a comprehensive understanding of differential metabolites and metabolic pathways between red wolfberry (Lycium barbarum L.) and yellow wolfberry (Lycium barbarum L. var. auranticarpum K.F.Ching), an analytical approach integrating ultra-performance liquid chromatography with tandem...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2024-12-01
|
| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024020267 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850117624620384256 |
|---|---|
| author | Tian LAN Guozhen DUAN Youchao QI Guanghui FAN Chunyan BAI Yede ZHANG |
| author_facet | Tian LAN Guozhen DUAN Youchao QI Guanghui FAN Chunyan BAI Yede ZHANG |
| author_sort | Tian LAN |
| collection | DOAJ |
| description | In the pursuit of a comprehensive understanding of differential metabolites and metabolic pathways between red wolfberry (Lycium barbarum L.) and yellow wolfberry (Lycium barbarum L. var. auranticarpum K.F.Ching), an analytical approach integrating ultra-performance liquid chromatography with tandem high-resolution mass spectrometry was employed, alongside a broadly targeted metabolomics strategy. Through multivariate statistical methods such as principal component analysis, orthogonal partial least squares discriminant analysis, and cluster analysis, under the conditions of fold change ≥2 or ≤0.5, variable importance projection value ≥1, and P<0.05, 202 differential metabolites (148 up-regulated and 54 down-regulated) were detected in red and yellow wolfberries under positive ion mode. A total of 240 differential metabolites (183 up-regulated and 57 down-regulated) were detected in the negative ion mode. Subsequent KEGG pathway enrichment analysis revealed that these differential metabolites were predominantly associated with secondary metabolite, tryptophan, glucosinolate, valine, isoleucine, leucine, and flavonoid biosynthesis pathways. The data suggested that the differential metabolites in Lycium barbarum L. and Lycium barbarum L. var. auranticarpum K.F.Ching were predominantly flavonoids, alkaloids, phenolic acids, amino acids, and their derivatives, with a general trend of up-regulation. These metabolites might be linked to the growth, development, disease resistance, and nutritional value of Lycium barbarum L. var. auranticarpum K.F.Ching, potentially through their involvement in tryptophan metabolism and biosynthesis pathways for valine, leucine, isoleucine, and flavonoids. The objective of this study was to contribute to the existing knowledge base on the nutritional value of these two wolfberry species and to offer a theoretical foundation for the refinement of wolfberry-related products through extensive metabolomics profiling. |
| format | Article |
| id | doaj-art-e7133735ce19479e954bf17e3d4e4834 |
| institution | OA Journals |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2024-12-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-e7133735ce19479e954bf17e3d4e48342025-08-20T02:36:03ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062024-12-01452492010.13386/j.issn1002-0306.20240202672024020267-24Extensively Targeted Metabolomics Analysis of Red and Yellow WolfberriesTian LAN0Guozhen DUAN1Youchao QI2Guanghui FAN3Chunyan BAI4Yede ZHANG5College of Agriculture and Forestry, Qinghai University, Xining 810016, ChinaCollege of Agriculture and Forestry, Qinghai University, Xining 810016, ChinaCollege of Agriculture and Forestry, Qinghai University, Xining 810016, ChinaCollege of Agriculture and Forestry, Qinghai University, Xining 810016, ChinaPing'an District Hospital of Traditional Chinese Medicine of Haidong City of Qinghai Province,Haidong 810699, ChinaQinghai Kunlun Wolfberry Industry Science and Technology Innovation Research Co., Ltd., Dulan 817000, ChinaIn the pursuit of a comprehensive understanding of differential metabolites and metabolic pathways between red wolfberry (Lycium barbarum L.) and yellow wolfberry (Lycium barbarum L. var. auranticarpum K.F.Ching), an analytical approach integrating ultra-performance liquid chromatography with tandem high-resolution mass spectrometry was employed, alongside a broadly targeted metabolomics strategy. Through multivariate statistical methods such as principal component analysis, orthogonal partial least squares discriminant analysis, and cluster analysis, under the conditions of fold change ≥2 or ≤0.5, variable importance projection value ≥1, and P<0.05, 202 differential metabolites (148 up-regulated and 54 down-regulated) were detected in red and yellow wolfberries under positive ion mode. A total of 240 differential metabolites (183 up-regulated and 57 down-regulated) were detected in the negative ion mode. Subsequent KEGG pathway enrichment analysis revealed that these differential metabolites were predominantly associated with secondary metabolite, tryptophan, glucosinolate, valine, isoleucine, leucine, and flavonoid biosynthesis pathways. The data suggested that the differential metabolites in Lycium barbarum L. and Lycium barbarum L. var. auranticarpum K.F.Ching were predominantly flavonoids, alkaloids, phenolic acids, amino acids, and their derivatives, with a general trend of up-regulation. These metabolites might be linked to the growth, development, disease resistance, and nutritional value of Lycium barbarum L. var. auranticarpum K.F.Ching, potentially through their involvement in tryptophan metabolism and biosynthesis pathways for valine, leucine, isoleucine, and flavonoids. The objective of this study was to contribute to the existing knowledge base on the nutritional value of these two wolfberry species and to offer a theoretical foundation for the refinement of wolfberry-related products through extensive metabolomics profiling.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024020267lycium barbarum l.lycium barbarum l. var. auranticarpum k.f.chingmetabolomicsdifferential metabolitesmetabolic pathways |
| spellingShingle | Tian LAN Guozhen DUAN Youchao QI Guanghui FAN Chunyan BAI Yede ZHANG Extensively Targeted Metabolomics Analysis of Red and Yellow Wolfberries Shipin gongye ke-ji lycium barbarum l. lycium barbarum l. var. auranticarpum k.f.ching metabolomics differential metabolites metabolic pathways |
| title | Extensively Targeted Metabolomics Analysis of Red and Yellow Wolfberries |
| title_full | Extensively Targeted Metabolomics Analysis of Red and Yellow Wolfberries |
| title_fullStr | Extensively Targeted Metabolomics Analysis of Red and Yellow Wolfberries |
| title_full_unstemmed | Extensively Targeted Metabolomics Analysis of Red and Yellow Wolfberries |
| title_short | Extensively Targeted Metabolomics Analysis of Red and Yellow Wolfberries |
| title_sort | extensively targeted metabolomics analysis of red and yellow wolfberries |
| topic | lycium barbarum l. lycium barbarum l. var. auranticarpum k.f.ching metabolomics differential metabolites metabolic pathways |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024020267 |
| work_keys_str_mv | AT tianlan extensivelytargetedmetabolomicsanalysisofredandyellowwolfberries AT guozhenduan extensivelytargetedmetabolomicsanalysisofredandyellowwolfberries AT youchaoqi extensivelytargetedmetabolomicsanalysisofredandyellowwolfberries AT guanghuifan extensivelytargetedmetabolomicsanalysisofredandyellowwolfberries AT chunyanbai extensivelytargetedmetabolomicsanalysisofredandyellowwolfberries AT yedezhang extensivelytargetedmetabolomicsanalysisofredandyellowwolfberries |