Neutralizing-antibody response to SARS-CoV-2 for 12 months after the COVID-19 workplace outbreaks in Japan.

This study aimed to elucidate the 12-month durability of neutralizing antibodies (NAbs) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in patients infected during the 2020 workplace outbreaks of coronavirus disease 2019 (COVID-19) in Japan. We followed 33 Japanese patients infe...

Full description

Saved in:
Bibliographic Details
Main Authors: Azumi Ishizaki, Xiuqiong Bi, Quynh Thi Nguyen, Tomomi Maeno, Akinori Hara, Hiroyuki Nakamura, Sanae Kuramoto, Koichi Nishi, Hiroyasu Ooe, Hiroshi Ichimura
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2022-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0273712&type=printable
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850023518571331584
author Azumi Ishizaki
Xiuqiong Bi
Quynh Thi Nguyen
Tomomi Maeno
Akinori Hara
Hiroyuki Nakamura
Sanae Kuramoto
Koichi Nishi
Hiroyasu Ooe
Hiroshi Ichimura
author_facet Azumi Ishizaki
Xiuqiong Bi
Quynh Thi Nguyen
Tomomi Maeno
Akinori Hara
Hiroyuki Nakamura
Sanae Kuramoto
Koichi Nishi
Hiroyasu Ooe
Hiroshi Ichimura
author_sort Azumi Ishizaki
collection DOAJ
description This study aimed to elucidate the 12-month durability of neutralizing antibodies (NAbs) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in patients infected during the 2020 workplace outbreaks of coronavirus disease 2019 (COVID-19) in Japan. We followed 33 Japanese patients infected with SARS-CoV-2 in April 2020 for 12 months (12M). Patients were tested for NAbs and for antibodies against the SARS-CoV-2 nucleocapsid (anti-NC-Ab) and antibodies against the spike receptor-binding domain (anti-RBD-Ab). Tests were performed at 2M, 6M, and 12M after the primary infection (api) with commercially available test kits. In 90.9% (30/33) of patients, NAbs persisted for 12M api, though the median titers significantly declined from 78.7% (interquartile range [IQR]: 73.0-85.0%) at 2M, to 59.8% (IQR: 51.2-77.9) at 6M (P = 0.008), and to 56.2% (IQR: 39.6-74.4) at 12M (P<0.001). An exponential decay model showed that the NAb level reached undetectable concentrations at 35.5 months api (95% confidence interval: 26.5-48.0 months). Additionally, NAb titers were significantly related to anti-RBD-Ab titers (rho = 0.736, P<0.001), but not to anti-NC-Ab titers. In most patients convalescing from COVID-19, NAbs persisted for 12M api. This result suggested that patients need a booster vaccination within one year api, even though NAbs could be detected for over two years api. Anti-RBD-Ab titers could be used as a surrogate marker for predicting residual NAb levels.
format Article
id doaj-art-e70f8568baae47b5bc5267b1143b03e3
institution DOAJ
issn 1932-6203
language English
publishDate 2022-01-01
publisher Public Library of Science (PLoS)
record_format Article
series PLoS ONE
spelling doaj-art-e70f8568baae47b5bc5267b1143b03e32025-08-20T03:01:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01178e027371210.1371/journal.pone.0273712Neutralizing-antibody response to SARS-CoV-2 for 12 months after the COVID-19 workplace outbreaks in Japan.Azumi IshizakiXiuqiong BiQuynh Thi NguyenTomomi MaenoAkinori HaraHiroyuki NakamuraSanae KuramotoKoichi NishiHiroyasu OoeHiroshi IchimuraThis study aimed to elucidate the 12-month durability of neutralizing antibodies (NAbs) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in patients infected during the 2020 workplace outbreaks of coronavirus disease 2019 (COVID-19) in Japan. We followed 33 Japanese patients infected with SARS-CoV-2 in April 2020 for 12 months (12M). Patients were tested for NAbs and for antibodies against the SARS-CoV-2 nucleocapsid (anti-NC-Ab) and antibodies against the spike receptor-binding domain (anti-RBD-Ab). Tests were performed at 2M, 6M, and 12M after the primary infection (api) with commercially available test kits. In 90.9% (30/33) of patients, NAbs persisted for 12M api, though the median titers significantly declined from 78.7% (interquartile range [IQR]: 73.0-85.0%) at 2M, to 59.8% (IQR: 51.2-77.9) at 6M (P = 0.008), and to 56.2% (IQR: 39.6-74.4) at 12M (P<0.001). An exponential decay model showed that the NAb level reached undetectable concentrations at 35.5 months api (95% confidence interval: 26.5-48.0 months). Additionally, NAb titers were significantly related to anti-RBD-Ab titers (rho = 0.736, P<0.001), but not to anti-NC-Ab titers. In most patients convalescing from COVID-19, NAbs persisted for 12M api. This result suggested that patients need a booster vaccination within one year api, even though NAbs could be detected for over two years api. Anti-RBD-Ab titers could be used as a surrogate marker for predicting residual NAb levels.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0273712&type=printable
spellingShingle Azumi Ishizaki
Xiuqiong Bi
Quynh Thi Nguyen
Tomomi Maeno
Akinori Hara
Hiroyuki Nakamura
Sanae Kuramoto
Koichi Nishi
Hiroyasu Ooe
Hiroshi Ichimura
Neutralizing-antibody response to SARS-CoV-2 for 12 months after the COVID-19 workplace outbreaks in Japan.
PLoS ONE
title Neutralizing-antibody response to SARS-CoV-2 for 12 months after the COVID-19 workplace outbreaks in Japan.
title_full Neutralizing-antibody response to SARS-CoV-2 for 12 months after the COVID-19 workplace outbreaks in Japan.
title_fullStr Neutralizing-antibody response to SARS-CoV-2 for 12 months after the COVID-19 workplace outbreaks in Japan.
title_full_unstemmed Neutralizing-antibody response to SARS-CoV-2 for 12 months after the COVID-19 workplace outbreaks in Japan.
title_short Neutralizing-antibody response to SARS-CoV-2 for 12 months after the COVID-19 workplace outbreaks in Japan.
title_sort neutralizing antibody response to sars cov 2 for 12 months after the covid 19 workplace outbreaks in japan
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0273712&type=printable
work_keys_str_mv AT azumiishizaki neutralizingantibodyresponsetosarscov2for12monthsafterthecovid19workplaceoutbreaksinjapan
AT xiuqiongbi neutralizingantibodyresponsetosarscov2for12monthsafterthecovid19workplaceoutbreaksinjapan
AT quynhthinguyen neutralizingantibodyresponsetosarscov2for12monthsafterthecovid19workplaceoutbreaksinjapan
AT tomomimaeno neutralizingantibodyresponsetosarscov2for12monthsafterthecovid19workplaceoutbreaksinjapan
AT akinorihara neutralizingantibodyresponsetosarscov2for12monthsafterthecovid19workplaceoutbreaksinjapan
AT hiroyukinakamura neutralizingantibodyresponsetosarscov2for12monthsafterthecovid19workplaceoutbreaksinjapan
AT sanaekuramoto neutralizingantibodyresponsetosarscov2for12monthsafterthecovid19workplaceoutbreaksinjapan
AT koichinishi neutralizingantibodyresponsetosarscov2for12monthsafterthecovid19workplaceoutbreaksinjapan
AT hiroyasuooe neutralizingantibodyresponsetosarscov2for12monthsafterthecovid19workplaceoutbreaksinjapan
AT hiroshiichimura neutralizingantibodyresponsetosarscov2for12monthsafterthecovid19workplaceoutbreaksinjapan