Natural variation of OsWRKY23 drives difference in nitrate use efficiency between indica and japonica rice
Abstract Between the two major rice subspecies, indica varieties generally exhibit higher nitrate (NO3 ‒) uptake and nitrogen (N)-use efficiency (NUE) than japonica varieties. Introducing efficient NO3 ‒ utilization alleles from indica into japonica could improve NUE, and at the same time uncover un...
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| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56752-7 |
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| author | Siyu Zhang Zhe Ji Wu Jiao Chengbo Shen Yaojun Qin Yunzhi Huang Menghan Huang Shuming Kang Xuan Liu Shunqi Li Zulong Mo Ying Yu Bingyu Jiang Yanan Tian Longfei Wang Qingxin Song Shaokui Wang Shan Li |
| author_facet | Siyu Zhang Zhe Ji Wu Jiao Chengbo Shen Yaojun Qin Yunzhi Huang Menghan Huang Shuming Kang Xuan Liu Shunqi Li Zulong Mo Ying Yu Bingyu Jiang Yanan Tian Longfei Wang Qingxin Song Shaokui Wang Shan Li |
| author_sort | Siyu Zhang |
| collection | DOAJ |
| description | Abstract Between the two major rice subspecies, indica varieties generally exhibit higher nitrate (NO3 ‒) uptake and nitrogen (N)-use efficiency (NUE) than japonica varieties. Introducing efficient NO3 ‒ utilization alleles from indica into japonica could improve NUE, and at the same time uncover unknown regulators of NO3 ‒ metabolism. Here, we identify OsWRKY23 as a key regulator of NO3 ‒ uptake and NUE differences between indica and japonica rice. The OsWRKY23 indica allele exhibits reduced transcriptional activation of a negative regulator of auxin accumulation, DULL NITROGEN RESPONSE1 (DNR1). The resultant increase in auxin level improves NO3 ‒ uptake and assimilation, which ultimately enhances grain yield. Geographical and evolutionary analyses reveal overlapping distribution of OsWRKY23 indica and DNR1 indica , particularly in low-fertility soils, suggesting their involvement in the adaptation to low N conditions to improve NUE and grain yield. Incorporating the OsWRKY23-DNR1 module from indica rice represents a promising strategy to enhance japonica NUE, which is crucial for sustainable agriculture. |
| format | Article |
| id | doaj-art-e70c8ace12b64203bfb790db2e0948e3 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e70c8ace12b64203bfb790db2e0948e32025-02-09T12:44:24ZengNature PortfolioNature Communications2041-17232025-02-0116111310.1038/s41467-025-56752-7Natural variation of OsWRKY23 drives difference in nitrate use efficiency between indica and japonica riceSiyu Zhang0Zhe Ji1Wu Jiao2Chengbo Shen3Yaojun Qin4Yunzhi Huang5Menghan Huang6Shuming Kang7Xuan Liu8Shunqi Li9Zulong Mo10Ying Yu11Bingyu Jiang12Yanan Tian13Longfei Wang14Qingxin Song15Shaokui Wang16Shan Li17State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural UniversityDepartment of Biology, University of OxfordState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural UniversityState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural UniversityAbstract Between the two major rice subspecies, indica varieties generally exhibit higher nitrate (NO3 ‒) uptake and nitrogen (N)-use efficiency (NUE) than japonica varieties. Introducing efficient NO3 ‒ utilization alleles from indica into japonica could improve NUE, and at the same time uncover unknown regulators of NO3 ‒ metabolism. Here, we identify OsWRKY23 as a key regulator of NO3 ‒ uptake and NUE differences between indica and japonica rice. The OsWRKY23 indica allele exhibits reduced transcriptional activation of a negative regulator of auxin accumulation, DULL NITROGEN RESPONSE1 (DNR1). The resultant increase in auxin level improves NO3 ‒ uptake and assimilation, which ultimately enhances grain yield. Geographical and evolutionary analyses reveal overlapping distribution of OsWRKY23 indica and DNR1 indica , particularly in low-fertility soils, suggesting their involvement in the adaptation to low N conditions to improve NUE and grain yield. Incorporating the OsWRKY23-DNR1 module from indica rice represents a promising strategy to enhance japonica NUE, which is crucial for sustainable agriculture.https://doi.org/10.1038/s41467-025-56752-7 |
| spellingShingle | Siyu Zhang Zhe Ji Wu Jiao Chengbo Shen Yaojun Qin Yunzhi Huang Menghan Huang Shuming Kang Xuan Liu Shunqi Li Zulong Mo Ying Yu Bingyu Jiang Yanan Tian Longfei Wang Qingxin Song Shaokui Wang Shan Li Natural variation of OsWRKY23 drives difference in nitrate use efficiency between indica and japonica rice Nature Communications |
| title | Natural variation of OsWRKY23 drives difference in nitrate use efficiency between indica and japonica rice |
| title_full | Natural variation of OsWRKY23 drives difference in nitrate use efficiency between indica and japonica rice |
| title_fullStr | Natural variation of OsWRKY23 drives difference in nitrate use efficiency between indica and japonica rice |
| title_full_unstemmed | Natural variation of OsWRKY23 drives difference in nitrate use efficiency between indica and japonica rice |
| title_short | Natural variation of OsWRKY23 drives difference in nitrate use efficiency between indica and japonica rice |
| title_sort | natural variation of oswrky23 drives difference in nitrate use efficiency between indica and japonica rice |
| url | https://doi.org/10.1038/s41467-025-56752-7 |
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