Characterization of CD4+ T Cell Subsets in Patients with Abdominal Aortic Aneurysms
Background. The mediators produced by CD4+ T lymphocytes are involved in the pathogenesis of aneurysmal lesions in abdominal aortic aneurysm (AAA) patients. The aim of this study was to identify and characterize the CD4+ T cell subsets involved in human AAA. Methods. The CD4+ T cell subsets in 30 hu...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/6967310 |
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| author | Fábio Haach Téo Rômulo Tadeu Dias de Oliveira Liana Villarejos Ronei Luciano Mamoni Albina Altemani Fabio Husemann Menezes Maria Heloisa Souza Lima Blotta |
| author_facet | Fábio Haach Téo Rômulo Tadeu Dias de Oliveira Liana Villarejos Ronei Luciano Mamoni Albina Altemani Fabio Husemann Menezes Maria Heloisa Souza Lima Blotta |
| author_sort | Fábio Haach Téo |
| collection | DOAJ |
| description | Background. The mediators produced by CD4+ T lymphocytes are involved in the pathogenesis of aneurysmal lesions in abdominal aortic aneurysm (AAA) patients. The aim of this study was to identify and characterize the CD4+ T cell subsets involved in human AAA. Methods. The CD4+ T cell subsets in 30 human aneurysmal lesions were determined using flow cytometry (FC) and immunohistochemistry (IHC). The peripheral blood mononuclear cells (PBMCs) from patients with AAA were also analyzed by FC and compared with control subjects. Results. Human aneurysmal lesions contained IFN-γ, IL-12p35, IL-4, IL-23p19, IL-17R, and IL-22 positive cells. PBMCs from AAA patients had higher expression levels of IFN-γ, TNF-α, IL-4, and IL-22 when compared to controls. Conclusions. Our results show the presence of TH1, TH2, TH17, and TH22 subsets in aneurysmal lesions of AAA patients and suggest that these cells may be mainly activated in situ, where they can induce tissue degradation and contribute to the pathogenesis of AAA. |
| format | Article |
| id | doaj-art-e703d51ac1eb4bb38370998e728112f1 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-e703d51ac1eb4bb38370998e728112f12025-08-20T03:26:10ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/69673106967310Characterization of CD4+ T Cell Subsets in Patients with Abdominal Aortic AneurysmsFábio Haach Téo0Rômulo Tadeu Dias de Oliveira1Liana Villarejos2Ronei Luciano Mamoni3Albina Altemani4Fabio Husemann Menezes5Maria Heloisa Souza Lima Blotta6Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, São Paulo 13083-887, BrazilDepartment of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, São Paulo 13083-887, BrazilDepartment of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, São Paulo 13083-887, BrazilDepartment of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, São Paulo 13083-887, BrazilDepartment of Pathology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, São Paulo 13083-887, BrazilDepartment of Surgery, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, São Paulo 13083-887, BrazilDepartment of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, São Paulo 13083-887, BrazilBackground. The mediators produced by CD4+ T lymphocytes are involved in the pathogenesis of aneurysmal lesions in abdominal aortic aneurysm (AAA) patients. The aim of this study was to identify and characterize the CD4+ T cell subsets involved in human AAA. Methods. The CD4+ T cell subsets in 30 human aneurysmal lesions were determined using flow cytometry (FC) and immunohistochemistry (IHC). The peripheral blood mononuclear cells (PBMCs) from patients with AAA were also analyzed by FC and compared with control subjects. Results. Human aneurysmal lesions contained IFN-γ, IL-12p35, IL-4, IL-23p19, IL-17R, and IL-22 positive cells. PBMCs from AAA patients had higher expression levels of IFN-γ, TNF-α, IL-4, and IL-22 when compared to controls. Conclusions. Our results show the presence of TH1, TH2, TH17, and TH22 subsets in aneurysmal lesions of AAA patients and suggest that these cells may be mainly activated in situ, where they can induce tissue degradation and contribute to the pathogenesis of AAA.http://dx.doi.org/10.1155/2018/6967310 |
| spellingShingle | Fábio Haach Téo Rômulo Tadeu Dias de Oliveira Liana Villarejos Ronei Luciano Mamoni Albina Altemani Fabio Husemann Menezes Maria Heloisa Souza Lima Blotta Characterization of CD4+ T Cell Subsets in Patients with Abdominal Aortic Aneurysms Mediators of Inflammation |
| title | Characterization of CD4+ T Cell Subsets in Patients with Abdominal Aortic Aneurysms |
| title_full | Characterization of CD4+ T Cell Subsets in Patients with Abdominal Aortic Aneurysms |
| title_fullStr | Characterization of CD4+ T Cell Subsets in Patients with Abdominal Aortic Aneurysms |
| title_full_unstemmed | Characterization of CD4+ T Cell Subsets in Patients with Abdominal Aortic Aneurysms |
| title_short | Characterization of CD4+ T Cell Subsets in Patients with Abdominal Aortic Aneurysms |
| title_sort | characterization of cd4 t cell subsets in patients with abdominal aortic aneurysms |
| url | http://dx.doi.org/10.1155/2018/6967310 |
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