CHITINOLYTIC ENZYMES IN THE RUMEN MICROBIAL ECOSYSTEM
Chitinolytic systems of rumen Clostridia (usually the most active rumen chitinolytic bacteria) and rumen anaerobic fungi were compared in the present study. The chitinolytic enzymes of tested Clostridia strains were represented mainly by extracellular exochitinases, N-acetylglucosaminidases, chitin...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
1998-12-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/17077 |
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| author | J. KOPEČNÝ Blanka HODROVÁ |
| author_facet | J. KOPEČNÝ Blanka HODROVÁ |
| author_sort | J. KOPEČNÝ |
| collection | DOAJ |
| description | Chitinolytic systems of rumen Clostridia (usually the most active rumen chitinolytic bacteria) and rumen anaerobic fungi were compared in the present study. The chitinolytic enzymes of tested Clostridia strains were represented mainly by extracellular exochitinases, N-acetylglucosaminidases, chitin deacetylases and chitosanases. Zymograms of exochitinases revealed that these enzymes were bound in complexes with MW higher than 100 kDa. Final products of chitin degradation were glucosamine, N-acetylglucosamine and chitin oligosaccharides. Fungal chitinases were represented mainly by extracellular endochitinases and chitin deacetylase in both mono- and polycentric fungi. The main activities in cellular fraction were chitosanase and endochitinase. There were no or low activity of N-acetylglucosaminidase activity in all fungal strains tested. In comparison with rumen chitinolytic bacteria there was lower production of exochitinases and no production of N-acetylglucosaminidase in rumen fungi. |
| format | Article |
| id | doaj-art-e6fa52aa3a994075882e4ee8185131cf |
| institution | OA Journals |
| issn | 1854-1941 |
| language | English |
| publishDate | 1998-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-e6fa52aa3a994075882e4ee8185131cf2025-08-20T02:04:48ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19411998-12-01721859310.14720/aas.1998.72.1.1707723471CHITINOLYTIC ENZYMES IN THE RUMEN MICROBIAL ECOSYSTEMJ. KOPEČNÝ0Blanka HODROVÁ1Czech Academy of Sciences, Institute of Animal Physiology and Genetics, Prague 10, Uhříněves, 104 00, Czech RepublicCzech Academy of Sciences, Institute of Animal Physiology and Genetics, Prague 10, Uhříněves, 104 00, Czech RepublicChitinolytic systems of rumen Clostridia (usually the most active rumen chitinolytic bacteria) and rumen anaerobic fungi were compared in the present study. The chitinolytic enzymes of tested Clostridia strains were represented mainly by extracellular exochitinases, N-acetylglucosaminidases, chitin deacetylases and chitosanases. Zymograms of exochitinases revealed that these enzymes were bound in complexes with MW higher than 100 kDa. Final products of chitin degradation were glucosamine, N-acetylglucosamine and chitin oligosaccharides. Fungal chitinases were represented mainly by extracellular endochitinases and chitin deacetylase in both mono- and polycentric fungi. The main activities in cellular fraction were chitosanase and endochitinase. There were no or low activity of N-acetylglucosaminidase activity in all fungal strains tested. In comparison with rumen chitinolytic bacteria there was lower production of exochitinases and no production of N-acetylglucosaminidase in rumen fungi.https://journals.uni-lj.si/aas/article/view/17077microbiologybacteriaclostridiumfungienzymeschitinolysisrumen |
| spellingShingle | J. KOPEČNÝ Blanka HODROVÁ CHITINOLYTIC ENZYMES IN THE RUMEN MICROBIAL ECOSYSTEM Acta Agriculturae Slovenica microbiology bacteria clostridium fungi enzymes chitinolysis rumen |
| title | CHITINOLYTIC ENZYMES IN THE RUMEN MICROBIAL ECOSYSTEM |
| title_full | CHITINOLYTIC ENZYMES IN THE RUMEN MICROBIAL ECOSYSTEM |
| title_fullStr | CHITINOLYTIC ENZYMES IN THE RUMEN MICROBIAL ECOSYSTEM |
| title_full_unstemmed | CHITINOLYTIC ENZYMES IN THE RUMEN MICROBIAL ECOSYSTEM |
| title_short | CHITINOLYTIC ENZYMES IN THE RUMEN MICROBIAL ECOSYSTEM |
| title_sort | chitinolytic enzymes in the rumen microbial ecosystem |
| topic | microbiology bacteria clostridium fungi enzymes chitinolysis rumen |
| url | https://journals.uni-lj.si/aas/article/view/17077 |
| work_keys_str_mv | AT jkopecny chitinolyticenzymesintherumenmicrobialecosystem AT blankahodrova chitinolyticenzymesintherumenmicrobialecosystem |