Licorice extract and carbenoxolone protect PC12 cells against serum/glucose deprivation-induced apoptosis through modulation of caspase-3 and PARP activation
Objective: Serum/glucose deprivation in cultured PC12 cells is considered an appropriate model for investigating detailed mechanisms of ischemia-induced brain injury. Here, we aimed to study the anti-apoptotic effects of licorice (Glycyrrhiza glabra L.) root extract and carbenoxolone on PC12 cells c...
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Mashhad University of Medical Sciences
2025-07-01
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| Series: | Avicenna Journal of Phytomedicine |
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| Online Access: | https://ajp.mums.ac.ir/article_25252_88e871836e1c45f0a35cd181990e2a28.pdf |
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| author | Hossein Hosseinzadeh Elham Ramazani Soheyla Bakhshi Zahra Tayarani Najjaran |
| author_facet | Hossein Hosseinzadeh Elham Ramazani Soheyla Bakhshi Zahra Tayarani Najjaran |
| author_sort | Hossein Hosseinzadeh |
| collection | DOAJ |
| description | Objective: Serum/glucose deprivation in cultured PC12 cells is considered an appropriate model for investigating detailed mechanisms of ischemia-induced brain injury. Here, we aimed to study the anti-apoptotic effects of licorice (Glycyrrhiza glabra L.) root extract and carbenoxolone on PC12 cells cultured in the serum/glucose deprivation (SGD) condition.Materials and Methods: Cells were incubated with the different concentrations of the G. glabra methanol extract (5-320 µg/ml) and carbenoxolone (0.5-32 µM) for 2 hr before being deprived of serum/glucose. Protection against cytotoxicity, increase in reactive oxygen species (ROS), and apoptosis was analyzed with resazurin, dichlorofluorescein diacetate (DCFH-DA), and western blot, respectively.Results: Serum/glucose deprivation induced cell death and apoptosis in PC12 cells. Pretreatment with the G. glabra methanol extract at 5-20 µg/ml and carbenoxolone at 0.5-2 µM for 2 hr significantly decreased the cytotoxicity (p<0.05), and pretreatment with the G. glabra methanol extract (5-160 µg/ml) and carbenoxolone (0.5 μM) significantly decreased the ROS content. Pretreatment with the G. glabra methanol extract and carbenoxolone at 5-20 µg/ml significantly prevented from the Poly (ADP-ribose) polymerase (PARP) and caspase-3 cleavage.Conclusion: Taken together, this study confirms the protective and free radical-scavenging potency of licorice extract and carbenoxolone in in vitro model of ischemia. Overall, it seems that pretreatment with the licorice extract and carbenoxolone may potentially slow the progression of brain ischemia. |
| format | Article |
| id | doaj-art-e6f9f6a0b98f4693b79e4a16d1342869 |
| institution | DOAJ |
| issn | 2228-7930 2228-7949 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj-art-e6f9f6a0b98f4693b79e4a16d13428692025-08-20T02:48:05ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492025-07-011541252126310.22038/ajp.2024.2525225252Licorice extract and carbenoxolone protect PC12 cells against serum/glucose deprivation-induced apoptosis through modulation of caspase-3 and PARP activationHossein Hosseinzadeh0Elham Ramazani1Soheyla Bakhshi2Zahra Tayarani Najjaran3Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.Department of Biology, Faculty of Science, Yazd University, Yazd, Iran. Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.Objective: Serum/glucose deprivation in cultured PC12 cells is considered an appropriate model for investigating detailed mechanisms of ischemia-induced brain injury. Here, we aimed to study the anti-apoptotic effects of licorice (Glycyrrhiza glabra L.) root extract and carbenoxolone on PC12 cells cultured in the serum/glucose deprivation (SGD) condition.Materials and Methods: Cells were incubated with the different concentrations of the G. glabra methanol extract (5-320 µg/ml) and carbenoxolone (0.5-32 µM) for 2 hr before being deprived of serum/glucose. Protection against cytotoxicity, increase in reactive oxygen species (ROS), and apoptosis was analyzed with resazurin, dichlorofluorescein diacetate (DCFH-DA), and western blot, respectively.Results: Serum/glucose deprivation induced cell death and apoptosis in PC12 cells. Pretreatment with the G. glabra methanol extract at 5-20 µg/ml and carbenoxolone at 0.5-2 µM for 2 hr significantly decreased the cytotoxicity (p<0.05), and pretreatment with the G. glabra methanol extract (5-160 µg/ml) and carbenoxolone (0.5 μM) significantly decreased the ROS content. Pretreatment with the G. glabra methanol extract and carbenoxolone at 5-20 µg/ml significantly prevented from the Poly (ADP-ribose) polymerase (PARP) and caspase-3 cleavage.Conclusion: Taken together, this study confirms the protective and free radical-scavenging potency of licorice extract and carbenoxolone in in vitro model of ischemia. Overall, it seems that pretreatment with the licorice extract and carbenoxolone may potentially slow the progression of brain ischemia.https://ajp.mums.ac.ir/article_25252_88e871836e1c45f0a35cd181990e2a28.pdflicorice extractcarbenoxoloneapoptosisischemiapc12 cells |
| spellingShingle | Hossein Hosseinzadeh Elham Ramazani Soheyla Bakhshi Zahra Tayarani Najjaran Licorice extract and carbenoxolone protect PC12 cells against serum/glucose deprivation-induced apoptosis through modulation of caspase-3 and PARP activation Avicenna Journal of Phytomedicine licorice extract carbenoxolone apoptosis ischemia pc12 cells |
| title | Licorice extract and carbenoxolone protect PC12 cells against serum/glucose deprivation-induced apoptosis through modulation of caspase-3 and PARP activation |
| title_full | Licorice extract and carbenoxolone protect PC12 cells against serum/glucose deprivation-induced apoptosis through modulation of caspase-3 and PARP activation |
| title_fullStr | Licorice extract and carbenoxolone protect PC12 cells against serum/glucose deprivation-induced apoptosis through modulation of caspase-3 and PARP activation |
| title_full_unstemmed | Licorice extract and carbenoxolone protect PC12 cells against serum/glucose deprivation-induced apoptosis through modulation of caspase-3 and PARP activation |
| title_short | Licorice extract and carbenoxolone protect PC12 cells against serum/glucose deprivation-induced apoptosis through modulation of caspase-3 and PARP activation |
| title_sort | licorice extract and carbenoxolone protect pc12 cells against serum glucose deprivation induced apoptosis through modulation of caspase 3 and parp activation |
| topic | licorice extract carbenoxolone apoptosis ischemia pc12 cells |
| url | https://ajp.mums.ac.ir/article_25252_88e871836e1c45f0a35cd181990e2a28.pdf |
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