Genome-wide identification and characterization of Dof gene family in Salvia miltiorrhiza
Salvia miltiorrhiza is an important medicinal plant, and its main secondary metabolites are tanshinones and phenolic acids. Dof transcription factors play an irreplaceable role in regulating plant growth and secondary metabolism. However, the characteristics of SmDof genes in S. miltiorrhiza have no...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Maximum Academic Press
2024-01-01
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| Series: | Ornamental Plant Research |
| Subjects: | |
| Online Access: | https://www.maxapress.com/article/doi/10.48130/opr-0024-0030 |
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| Summary: | Salvia miltiorrhiza is an important medicinal plant, and its main secondary metabolites are tanshinones and phenolic acids. Dof transcription factors play an irreplaceable role in regulating plant growth and secondary metabolism. However, the characteristics of SmDof genes in S. miltiorrhiza have not yet been studied. Based on the whole genome data of S. miltiorrhiza, the SmDofs family has been systematically explored. A total of 31 Dof members have been identified in S. miltiorrhiza, and they are clustered into five subgroups according to their evolutionary relationships. Co-expression network results indicated that two SmDof genes (SmDof12 and SmDof29) might be involved in modulating the biosynthesis of phenolic acids or tanshinones. To investigate this hypothesis, dual luciferase experiments was introduced to examine the downstream target gene of SmDof12 and SmDof29. Subsequently, it was validated that SmDof12 inhibited the transcription of the SmGGPPS promoter, and SmDof29 significantly activated the SmPAL promoter. The present studies offers important data about the underlying function of SmDof12 and SmDof29 involved in the biosynthesis of phenolic acid or tanshinone, and provides valuable insights into further research of the SmDof gene families in S. miltiorrhiza. |
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| ISSN: | 2769-2094 |