Molecular Characterization of the Porcine Group A Rotavirus NSP2 and NSP5/6 Genes from São Paulo State, Brazil, in 2011/12
Rotaviruses are responsible for the acute diarrhea in various mammalian and avian species. The nonstructural proteins NSP2 and NSP5 are involved in the rotavirus replication and the formation of viroplasm, cytoplasmic inclusion bodies within which new viral particles morphogenesis and viral RNA repl...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/241686 |
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| author | Bruna Rocha Passos Barbosa Nara Thiers Cacciatori Galleti Bernardes Laila Andreia Rodrigues Beserra Fábio Gregori |
| author_facet | Bruna Rocha Passos Barbosa Nara Thiers Cacciatori Galleti Bernardes Laila Andreia Rodrigues Beserra Fábio Gregori |
| author_sort | Bruna Rocha Passos Barbosa |
| collection | DOAJ |
| description | Rotaviruses are responsible for the acute diarrhea in various mammalian and avian species. The nonstructural proteins NSP2 and NSP5 are involved in the rotavirus replication and the formation of viroplasm, cytoplasmic inclusion bodies within which new viral particles morphogenesis and viral RNA replication occur. There are few studies on the genetic diversity of those proteins; thus this study aims at characterizing the diversity of rotavirus based on NSP2 and NSP5 genes in rotaviruses circulating in Brazilian pig farms. For this purpose, 63 fecal samples from pig farms located in six different cities in the São Paulo State, Brazil, were screened by nested RT-PCR. Seven strains had the partial nucleotide sequencing for NSP2, whereas in six, the total sequencing for NSP5. All were characterized as genotype H1 and N1. The nucleotide identity of NSP2 genes ranged from 100% to 86.4% and the amino acid identity from 100% to 91.5%. For NSP5, the nucleotide identity was from 100% to 95.1% and the amino acid identity from 100% to 97.4%. It is concluded that the genotypes of the strains circulating in the region of study are in agreement with those reported in the literature for swine and that there is the possibility of interaction between human and animal rotaviruses. |
| format | Article |
| id | doaj-art-e6f6d072bd8e4a438fd12bd962ab1f19 |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-e6f6d072bd8e4a438fd12bd962ab1f192025-08-20T02:21:10ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/241686241686Molecular Characterization of the Porcine Group A Rotavirus NSP2 and NSP5/6 Genes from São Paulo State, Brazil, in 2011/12Bruna Rocha Passos Barbosa0Nara Thiers Cacciatori Galleti Bernardes1Laila Andreia Rodrigues Beserra2Fábio Gregori3Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SD, BrazilDepartment of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SD, BrazilDepartment of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SD, BrazilDepartment of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SD, BrazilRotaviruses are responsible for the acute diarrhea in various mammalian and avian species. The nonstructural proteins NSP2 and NSP5 are involved in the rotavirus replication and the formation of viroplasm, cytoplasmic inclusion bodies within which new viral particles morphogenesis and viral RNA replication occur. There are few studies on the genetic diversity of those proteins; thus this study aims at characterizing the diversity of rotavirus based on NSP2 and NSP5 genes in rotaviruses circulating in Brazilian pig farms. For this purpose, 63 fecal samples from pig farms located in six different cities in the São Paulo State, Brazil, were screened by nested RT-PCR. Seven strains had the partial nucleotide sequencing for NSP2, whereas in six, the total sequencing for NSP5. All were characterized as genotype H1 and N1. The nucleotide identity of NSP2 genes ranged from 100% to 86.4% and the amino acid identity from 100% to 91.5%. For NSP5, the nucleotide identity was from 100% to 95.1% and the amino acid identity from 100% to 97.4%. It is concluded that the genotypes of the strains circulating in the region of study are in agreement with those reported in the literature for swine and that there is the possibility of interaction between human and animal rotaviruses.http://dx.doi.org/10.1155/2013/241686 |
| spellingShingle | Bruna Rocha Passos Barbosa Nara Thiers Cacciatori Galleti Bernardes Laila Andreia Rodrigues Beserra Fábio Gregori Molecular Characterization of the Porcine Group A Rotavirus NSP2 and NSP5/6 Genes from São Paulo State, Brazil, in 2011/12 The Scientific World Journal |
| title | Molecular Characterization of the Porcine Group A Rotavirus NSP2 and NSP5/6 Genes from São Paulo State, Brazil, in 2011/12 |
| title_full | Molecular Characterization of the Porcine Group A Rotavirus NSP2 and NSP5/6 Genes from São Paulo State, Brazil, in 2011/12 |
| title_fullStr | Molecular Characterization of the Porcine Group A Rotavirus NSP2 and NSP5/6 Genes from São Paulo State, Brazil, in 2011/12 |
| title_full_unstemmed | Molecular Characterization of the Porcine Group A Rotavirus NSP2 and NSP5/6 Genes from São Paulo State, Brazil, in 2011/12 |
| title_short | Molecular Characterization of the Porcine Group A Rotavirus NSP2 and NSP5/6 Genes from São Paulo State, Brazil, in 2011/12 |
| title_sort | molecular characterization of the porcine group a rotavirus nsp2 and nsp5 6 genes from sao paulo state brazil in 2011 12 |
| url | http://dx.doi.org/10.1155/2013/241686 |
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