Effects of arabinoxylan on BDNF/TrkB/p-CREB signaling pathway in the prefrontal cortex and intestinal microbiome in post-stroke depressed rats
Abstract Aim To explore the effects of arabinoxylan on the BDNF/TrkB/p-CREB signaling pathway in the prefrontal cortex of post-stroke depressed rats, and to explore its neuronal protective effects through the microbial-gut-brain axis in the regulation of this pathway. Methods The rat model of post-s...
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| Format: | Article |
| Language: | English |
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BMC
2025-07-01
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| Series: | BMC Neuroscience |
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| Online Access: | https://doi.org/10.1186/s12868-025-00964-6 |
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| author | Bin-yu Bi Lin Lin Liu Huang Jun Zhou Wei-juan Yan Ling Huang Jie Wang Xue-bin Li |
| author_facet | Bin-yu Bi Lin Lin Liu Huang Jun Zhou Wei-juan Yan Ling Huang Jie Wang Xue-bin Li |
| author_sort | Bin-yu Bi |
| collection | DOAJ |
| description | Abstract Aim To explore the effects of arabinoxylan on the BDNF/TrkB/p-CREB signaling pathway in the prefrontal cortex of post-stroke depressed rats, and to explore its neuronal protective effects through the microbial-gut-brain axis in the regulation of this pathway. Methods The rat model of post-stroke depression (PSD) was established by middle cerebral artery occlusion (MCAO) combined with chronic unpredictable mild stimulation (CUMS). They were randomly divided into 5 groups (blank control, post-stroke depression, arabinoxylan, fluoxetine hydrochloride, fluoxetine hydrochloride combined arabinoxylan). The rats were treated differently for 28 days according to their grouping. Body mass, sugar and water consumption experiments and open-field experiments were used to evaluate the behavior of rats. The pathological changes were observed by H&E staining. The expression levels of amine neurotransmitters were detected by ELISA. The expression levels of BDNF mRNA and BDNF, TrkB and p-CREB were detected by RT-PCR and Western blot. The analysis of intestinal metagenomics was conducted by 16 S rDNA sequencing. Results Compared with the post-stroke depression group, the body weight, activity and sugar water consumption rate of the arabinoxylan group were increased. The expression levels of 5-HT in the prefrontal cortex, colon and serum levels of 5-HT, DA and NE were increased. The expression levels of BDNF mRNA and BDNF, TrkB and P-CREB in the prefrontal cortex were also upregulated. The number of neurons in the prefrontal cortex increased; Colon mucosal injury and inflammatory cell infiltration decreased, the intestinal microbial diversity increased; The relative abundance of probiotics such as bifidobacterium, Christensenia, Dubosiella New York and ruminococcus increased. The relative abundance of Prevotella NK3B31 group was reduced. The level of 5-HT in the prefrontal cortex was negatively correlated with the abundance of Prevotellaceae NK3B31 group. Conclusion Arabinoxylan improved depressive-like behavior in rats and its neuroprotective role was achieved by promoting the growth of intestinal probiotics, improving the intestinal barrier, affecting the BDNF/TrkB/p-CREB signaling pathway, and increasing the expression levels of monoamine neurotransmitters 5-HT, DA and NE. |
| format | Article |
| id | doaj-art-e6f368f3f67e4890bac733dae1777753 |
| institution | Kabale University |
| issn | 1471-2202 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neuroscience |
| spelling | doaj-art-e6f368f3f67e4890bac733dae17777532025-08-20T03:42:26ZengBMCBMC Neuroscience1471-22022025-07-0126111410.1186/s12868-025-00964-6Effects of arabinoxylan on BDNF/TrkB/p-CREB signaling pathway in the prefrontal cortex and intestinal microbiome in post-stroke depressed ratsBin-yu Bi0Lin Lin1Liu Huang2Jun Zhou3Wei-juan Yan4Ling Huang5Jie Wang6Xue-bin Li7Youjiang Medical University for NationalitiesYoujiang Medical University for NationalitiesYoujiang Medical University for NationalitiesYoujiang Medical University for NationalitiesYoujiang Medical University for NationalitiesYoujiang Medical University for NationalitiesYoujiang Medical University for Nationalities Affiliated HospitalYoujiang Medical University for Nationalities Affiliated HospitalAbstract Aim To explore the effects of arabinoxylan on the BDNF/TrkB/p-CREB signaling pathway in the prefrontal cortex of post-stroke depressed rats, and to explore its neuronal protective effects through the microbial-gut-brain axis in the regulation of this pathway. Methods The rat model of post-stroke depression (PSD) was established by middle cerebral artery occlusion (MCAO) combined with chronic unpredictable mild stimulation (CUMS). They were randomly divided into 5 groups (blank control, post-stroke depression, arabinoxylan, fluoxetine hydrochloride, fluoxetine hydrochloride combined arabinoxylan). The rats were treated differently for 28 days according to their grouping. Body mass, sugar and water consumption experiments and open-field experiments were used to evaluate the behavior of rats. The pathological changes were observed by H&E staining. The expression levels of amine neurotransmitters were detected by ELISA. The expression levels of BDNF mRNA and BDNF, TrkB and p-CREB were detected by RT-PCR and Western blot. The analysis of intestinal metagenomics was conducted by 16 S rDNA sequencing. Results Compared with the post-stroke depression group, the body weight, activity and sugar water consumption rate of the arabinoxylan group were increased. The expression levels of 5-HT in the prefrontal cortex, colon and serum levels of 5-HT, DA and NE were increased. The expression levels of BDNF mRNA and BDNF, TrkB and P-CREB in the prefrontal cortex were also upregulated. The number of neurons in the prefrontal cortex increased; Colon mucosal injury and inflammatory cell infiltration decreased, the intestinal microbial diversity increased; The relative abundance of probiotics such as bifidobacterium, Christensenia, Dubosiella New York and ruminococcus increased. The relative abundance of Prevotella NK3B31 group was reduced. The level of 5-HT in the prefrontal cortex was negatively correlated with the abundance of Prevotellaceae NK3B31 group. Conclusion Arabinoxylan improved depressive-like behavior in rats and its neuroprotective role was achieved by promoting the growth of intestinal probiotics, improving the intestinal barrier, affecting the BDNF/TrkB/p-CREB signaling pathway, and increasing the expression levels of monoamine neurotransmitters 5-HT, DA and NE.https://doi.org/10.1186/s12868-025-00964-6Post-stroke depressionArabinoxylanBDNF/TrkB/p-CREB signaling pathwayGut microbesMonoamine neurotransmittersBehavior |
| spellingShingle | Bin-yu Bi Lin Lin Liu Huang Jun Zhou Wei-juan Yan Ling Huang Jie Wang Xue-bin Li Effects of arabinoxylan on BDNF/TrkB/p-CREB signaling pathway in the prefrontal cortex and intestinal microbiome in post-stroke depressed rats BMC Neuroscience Post-stroke depression Arabinoxylan BDNF/TrkB/p-CREB signaling pathway Gut microbes Monoamine neurotransmitters Behavior |
| title | Effects of arabinoxylan on BDNF/TrkB/p-CREB signaling pathway in the prefrontal cortex and intestinal microbiome in post-stroke depressed rats |
| title_full | Effects of arabinoxylan on BDNF/TrkB/p-CREB signaling pathway in the prefrontal cortex and intestinal microbiome in post-stroke depressed rats |
| title_fullStr | Effects of arabinoxylan on BDNF/TrkB/p-CREB signaling pathway in the prefrontal cortex and intestinal microbiome in post-stroke depressed rats |
| title_full_unstemmed | Effects of arabinoxylan on BDNF/TrkB/p-CREB signaling pathway in the prefrontal cortex and intestinal microbiome in post-stroke depressed rats |
| title_short | Effects of arabinoxylan on BDNF/TrkB/p-CREB signaling pathway in the prefrontal cortex and intestinal microbiome in post-stroke depressed rats |
| title_sort | effects of arabinoxylan on bdnf trkb p creb signaling pathway in the prefrontal cortex and intestinal microbiome in post stroke depressed rats |
| topic | Post-stroke depression Arabinoxylan BDNF/TrkB/p-CREB signaling pathway Gut microbes Monoamine neurotransmitters Behavior |
| url | https://doi.org/10.1186/s12868-025-00964-6 |
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