Optimization of Starch Saccharification Process and Structural Characterization of Starch Using Low Transglycosidation Activity Glucoamylase GluM3
This paper discussed the optimization of the corn starch saccharification process using the glucoamylase GluM3, and explored its impact on the structure of starch granules to further promote the industrial application of GluM3 in starch sugar production. The study first determined the optimal saccha...
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The editorial department of Science and Technology of Food Industry
2025-02-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024010041 |
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| author | Haiyue FAN Zhao YU Zhengjie ZHANG Ming LI Yingguo BAI Feng YU Zhongyuan LI |
| author_facet | Haiyue FAN Zhao YU Zhengjie ZHANG Ming LI Yingguo BAI Feng YU Zhongyuan LI |
| author_sort | Haiyue FAN |
| collection | DOAJ |
| description | This paper discussed the optimization of the corn starch saccharification process using the glucoamylase GluM3, and explored its impact on the structure of starch granules to further promote the industrial application of GluM3 in starch sugar production. The study first determined the optimal saccharification process for low transglycosylation activity glucoamylase GluM3 through single-factor experiments and response surface experiments. Structural characterization of starch granules was conducted using methods such as scanning electron microscopy, X-ray diffraction, and infrared spectroscopy. The results showed that the optimal saccharification conditions were a liquefaction DE value of 16%, temperature at 66 ℃, pH at 3.7, time at 3 h, enzyme dosage at 450 U/g with pullulanase dosage at 1.25 U/g. After optimization, the glucose content (DX value) in corn starch saccharification liquid reached 92.68%. After saccharification reaction, corn starch changed from smooth intact particles to fragmented debris state. There was a certain degree of damage to internal crystalline structure during saccharification. A decrease in short-range order degree after saccharification reaction was observed. Furthermore, various maltodextrins were produced after corn starch enzymatic hydrolysis reaction while maltodextrins were further converted into glucose by GluM3 with a proportion as high as 99.34%. This study establishes the optimal saccharification process for low transglycosylation activity GluM3 on corn starch and investigates changes in microstructure and physical properties during the saccharification process, providing theoretical basis for subsequent industrial applications of GluM3. |
| format | Article |
| id | doaj-art-e6f063e37ac54052a5f3695af7ae7e7f |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-e6f063e37ac54052a5f3695af7ae7e7f2025-01-21T07:24:08ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-02-0146320421210.13386/j.issn1002-0306.20240100412024010041-3Optimization of Starch Saccharification Process and Structural Characterization of Starch Using Low Transglycosidation Activity Glucoamylase GluM3Haiyue FAN0Zhao YU1Zhengjie ZHANG2Ming LI3Yingguo BAI4Feng YU5Zhongyuan LI6College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaCollege of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaCollege of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaCollege of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaBeijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaBaiyin Sino Biotechnology Co., Ltd., Baiyin 730900, ChinaCollege of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaThis paper discussed the optimization of the corn starch saccharification process using the glucoamylase GluM3, and explored its impact on the structure of starch granules to further promote the industrial application of GluM3 in starch sugar production. The study first determined the optimal saccharification process for low transglycosylation activity glucoamylase GluM3 through single-factor experiments and response surface experiments. Structural characterization of starch granules was conducted using methods such as scanning electron microscopy, X-ray diffraction, and infrared spectroscopy. The results showed that the optimal saccharification conditions were a liquefaction DE value of 16%, temperature at 66 ℃, pH at 3.7, time at 3 h, enzyme dosage at 450 U/g with pullulanase dosage at 1.25 U/g. After optimization, the glucose content (DX value) in corn starch saccharification liquid reached 92.68%. After saccharification reaction, corn starch changed from smooth intact particles to fragmented debris state. There was a certain degree of damage to internal crystalline structure during saccharification. A decrease in short-range order degree after saccharification reaction was observed. Furthermore, various maltodextrins were produced after corn starch enzymatic hydrolysis reaction while maltodextrins were further converted into glucose by GluM3 with a proportion as high as 99.34%. This study establishes the optimal saccharification process for low transglycosylation activity GluM3 on corn starch and investigates changes in microstructure and physical properties during the saccharification process, providing theoretical basis for subsequent industrial applications of GluM3.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024010041corn starchglucoamylaseoptimization of saccharification processstructural characterization |
| spellingShingle | Haiyue FAN Zhao YU Zhengjie ZHANG Ming LI Yingguo BAI Feng YU Zhongyuan LI Optimization of Starch Saccharification Process and Structural Characterization of Starch Using Low Transglycosidation Activity Glucoamylase GluM3 Shipin gongye ke-ji corn starch glucoamylase optimization of saccharification process structural characterization |
| title | Optimization of Starch Saccharification Process and Structural Characterization of Starch Using Low Transglycosidation Activity Glucoamylase GluM3 |
| title_full | Optimization of Starch Saccharification Process and Structural Characterization of Starch Using Low Transglycosidation Activity Glucoamylase GluM3 |
| title_fullStr | Optimization of Starch Saccharification Process and Structural Characterization of Starch Using Low Transglycosidation Activity Glucoamylase GluM3 |
| title_full_unstemmed | Optimization of Starch Saccharification Process and Structural Characterization of Starch Using Low Transglycosidation Activity Glucoamylase GluM3 |
| title_short | Optimization of Starch Saccharification Process and Structural Characterization of Starch Using Low Transglycosidation Activity Glucoamylase GluM3 |
| title_sort | optimization of starch saccharification process and structural characterization of starch using low transglycosidation activity glucoamylase glum3 |
| topic | corn starch glucoamylase optimization of saccharification process structural characterization |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024010041 |
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