Construction of high-yield inositol-1-phosphate synthase recombinant Escherichia coli and optimization of fermentation conditions for myo-inositol production
In this study, the recombinant plasmid pETDuet-INO1 was constructed by introducing the expression gene INO1 of inositol-1-phosphate synthase (IPS) (a key enzyme in inositol synthesis pathway) cloned from Saccharomyces cerevisiae SC288 by gene engineering technology into plasmid pETDuet-1. The recomb...
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Editorial Department of China Brewing
2024-12-01
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| Series: | Zhongguo niangzao |
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| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-12-143.pdf |
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| author | YI Minghua, REN Guoping, HU Qiong, QIAN Mengmeng |
| author_facet | YI Minghua, REN Guoping, HU Qiong, QIAN Mengmeng |
| author_sort | YI Minghua, REN Guoping, HU Qiong, QIAN Mengmeng |
| collection | DOAJ |
| description | In this study, the recombinant plasmid pETDuet-INO1 was constructed by introducing the expression gene INO1 of inositol-1-phosphate synthase (IPS) (a key enzyme in inositol synthesis pathway) cloned from Saccharomyces cerevisiae SC288 by gene engineering technology into plasmid pETDuet-1. The recombinant strain Escherichia coli BL21/pETDuet-INO1 was constructed by introducing the recombinant plasmid pETDuet- INO1 into E. coli BL21 to achieve efficient expression of IPS in E. coli. The fermentation conditions for myo-inositol production were optimized by single factor experiments and orthogonal experiments. The results showed that the recombinant E. coli BL21/pETDuet-INO1 was successfully constructed, and the optimal fermentation conditions for myo-inositol production were as follows: initial glucose addition 12 g/L, induction temperature 28 ℃, and inoculum 3%. The yield of myo-inositol was (0.81±0.007) g/L after induction 24 h under these conditions, which was 60.39% higher than that before optimization. |
| format | Article |
| id | doaj-art-e6ed7a996aa840d7ad00d7deef5ffb53 |
| institution | OA Journals |
| issn | 0254-5071 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Editorial Department of China Brewing |
| record_format | Article |
| series | Zhongguo niangzao |
| spelling | doaj-art-e6ed7a996aa840d7ad00d7deef5ffb532025-08-20T02:11:05ZengEditorial Department of China BrewingZhongguo niangzao0254-50712024-12-01431214314810.11882/j.issn.0254-5071.2024.12.021Construction of high-yield inositol-1-phosphate synthase recombinant Escherichia coli and optimization of fermentation conditions for myo-inositol productionYI Minghua, REN Guoping, HU Qiong, QIAN Mengmeng01. Department of Health and Tourism, Hangzhou Wanxiang Polytechnic, Hangzhou 310014, China; ;2. Research Center of Good Hangzhou, Hangzhou Wanxiang Polytechnic, Hangzhou 310014, ChinaIn this study, the recombinant plasmid pETDuet-INO1 was constructed by introducing the expression gene INO1 of inositol-1-phosphate synthase (IPS) (a key enzyme in inositol synthesis pathway) cloned from Saccharomyces cerevisiae SC288 by gene engineering technology into plasmid pETDuet-1. The recombinant strain Escherichia coli BL21/pETDuet-INO1 was constructed by introducing the recombinant plasmid pETDuet- INO1 into E. coli BL21 to achieve efficient expression of IPS in E. coli. The fermentation conditions for myo-inositol production were optimized by single factor experiments and orthogonal experiments. The results showed that the recombinant E. coli BL21/pETDuet-INO1 was successfully constructed, and the optimal fermentation conditions for myo-inositol production were as follows: initial glucose addition 12 g/L, induction temperature 28 ℃, and inoculum 3%. The yield of myo-inositol was (0.81±0.007) g/L after induction 24 h under these conditions, which was 60.39% higher than that before optimization.https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-12-143.pdfescherichia coli|inositol-1-phosphate synthase|genetic engineering|myo-inositol|orthogonal experiment|fermentation condition |
| spellingShingle | YI Minghua, REN Guoping, HU Qiong, QIAN Mengmeng Construction of high-yield inositol-1-phosphate synthase recombinant Escherichia coli and optimization of fermentation conditions for myo-inositol production Zhongguo niangzao escherichia coli|inositol-1-phosphate synthase|genetic engineering|myo-inositol|orthogonal experiment|fermentation condition |
| title | Construction of high-yield inositol-1-phosphate synthase recombinant Escherichia coli and optimization of fermentation conditions for myo-inositol production |
| title_full | Construction of high-yield inositol-1-phosphate synthase recombinant Escherichia coli and optimization of fermentation conditions for myo-inositol production |
| title_fullStr | Construction of high-yield inositol-1-phosphate synthase recombinant Escherichia coli and optimization of fermentation conditions for myo-inositol production |
| title_full_unstemmed | Construction of high-yield inositol-1-phosphate synthase recombinant Escherichia coli and optimization of fermentation conditions for myo-inositol production |
| title_short | Construction of high-yield inositol-1-phosphate synthase recombinant Escherichia coli and optimization of fermentation conditions for myo-inositol production |
| title_sort | construction of high yield inositol 1 phosphate synthase recombinant escherichia coli and optimization of fermentation conditions for myo inositol production |
| topic | escherichia coli|inositol-1-phosphate synthase|genetic engineering|myo-inositol|orthogonal experiment|fermentation condition |
| url | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-12-143.pdf |
| work_keys_str_mv | AT yiminghuarenguopinghuqiongqianmengmeng constructionofhighyieldinositol1phosphatesynthaserecombinantescherichiacoliandoptimizationoffermentationconditionsformyoinositolproduction |