Construction of high-yield inositol-1-phosphate synthase recombinant Escherichia coli and optimization of fermentation conditions for myo-inositol production
In this study, the recombinant plasmid pETDuet-INO1 was constructed by introducing the expression gene INO1 of inositol-1-phosphate synthase (IPS) (a key enzyme in inositol synthesis pathway) cloned from Saccharomyces cerevisiae SC288 by gene engineering technology into plasmid pETDuet-1. The recomb...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Editorial Department of China Brewing
2024-12-01
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| Series: | Zhongguo niangzao |
| Subjects: | |
| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-12-143.pdf |
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| Summary: | In this study, the recombinant plasmid pETDuet-INO1 was constructed by introducing the expression gene INO1 of inositol-1-phosphate synthase (IPS) (a key enzyme in inositol synthesis pathway) cloned from Saccharomyces cerevisiae SC288 by gene engineering technology into plasmid pETDuet-1. The recombinant strain Escherichia coli BL21/pETDuet-INO1 was constructed by introducing the recombinant plasmid pETDuet- INO1 into E. coli BL21 to achieve efficient expression of IPS in E. coli. The fermentation conditions for myo-inositol production were optimized by single factor experiments and orthogonal experiments. The results showed that the recombinant E. coli BL21/pETDuet-INO1 was successfully constructed, and the optimal fermentation conditions for myo-inositol production were as follows: initial glucose addition 12 g/L, induction temperature 28 ℃, and inoculum 3%. The yield of myo-inositol was (0.81±0.007) g/L after induction 24 h under these conditions, which was 60.39% higher than that before optimization. |
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| ISSN: | 0254-5071 |