Evaluation of the parameters affecting the loading of anticancer drug Paclitaxel on coated gold nanoparticles for breast cancer treatment
Abstract The purpose of this study is the design and synthesis of gold nanoparticles (GNPs) conjugated with paclitaxel and to investigate the parameters affecting the stability of synthesised nanoparticles with drug delivery capability. Here, synthesised GNPs were coated with polyethylene glycol. Th...
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| Format: | Article |
| Language: | English |
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Wiley
2023-05-01
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| Series: | IET Nanobiotechnology |
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| Online Access: | https://doi.org/10.1049/nbt2.12121 |
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| author | Afrooz kharazmi Neda Attaran |
| author_facet | Afrooz kharazmi Neda Attaran |
| author_sort | Afrooz kharazmi |
| collection | DOAJ |
| description | Abstract The purpose of this study is the design and synthesis of gold nanoparticles (GNPs) conjugated with paclitaxel and to investigate the parameters affecting the stability of synthesised nanoparticles with drug delivery capability. Here, synthesised GNPs were coated with polyethylene glycol. Then these particles were conjugated with paclitaxel under different conditions and the physical and structural characteristics, as well as the factors affecting the loading of paclitaxel on nanoparticles, were evaluated by ultraviolet spectrophotometer, fourier transform infrared spectroscopy, transmission electron microscopy, dynamic light scattering and zeta potential apparatus. It was found that pegylated GNPs have a limited loading capacity at the time of 24 h of incubation and the Paclitaxel loading was observed to be pH dependent. The use of these particles in the treatment of breast cancer (MCF7) was also investigated using the MTT test. It was determined that the survival percentage of MCF7 cells in the presence of paclitaxel‐bound nanoparticles decreases to about 55% at the maximum measured concentration (690 μM). |
| format | Article |
| id | doaj-art-e6ead9556be0444d8b622609bfadfc9e |
| institution | Kabale University |
| issn | 1751-8741 1751-875X |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj-art-e6ead9556be0444d8b622609bfadfc9e2025-08-20T03:26:11ZengWileyIET Nanobiotechnology1751-87411751-875X2023-05-0117323424510.1049/nbt2.12121Evaluation of the parameters affecting the loading of anticancer drug Paclitaxel on coated gold nanoparticles for breast cancer treatmentAfrooz kharazmi0Neda Attaran1Department of Biomedical Engineering Applied Biophotonics Research Center Tehran Science and Research Branch Islamic Azad University Tehran IranDepartment of Medical Nanotechnology Applied Biophotonics Research Center Tehran Science and Research Branch Islamic Azad University Tehran IranAbstract The purpose of this study is the design and synthesis of gold nanoparticles (GNPs) conjugated with paclitaxel and to investigate the parameters affecting the stability of synthesised nanoparticles with drug delivery capability. Here, synthesised GNPs were coated with polyethylene glycol. Then these particles were conjugated with paclitaxel under different conditions and the physical and structural characteristics, as well as the factors affecting the loading of paclitaxel on nanoparticles, were evaluated by ultraviolet spectrophotometer, fourier transform infrared spectroscopy, transmission electron microscopy, dynamic light scattering and zeta potential apparatus. It was found that pegylated GNPs have a limited loading capacity at the time of 24 h of incubation and the Paclitaxel loading was observed to be pH dependent. The use of these particles in the treatment of breast cancer (MCF7) was also investigated using the MTT test. It was determined that the survival percentage of MCF7 cells in the presence of paclitaxel‐bound nanoparticles decreases to about 55% at the maximum measured concentration (690 μM).https://doi.org/10.1049/nbt2.12121cancernanomedicinenanoparticles |
| spellingShingle | Afrooz kharazmi Neda Attaran Evaluation of the parameters affecting the loading of anticancer drug Paclitaxel on coated gold nanoparticles for breast cancer treatment IET Nanobiotechnology cancer nanomedicine nanoparticles |
| title | Evaluation of the parameters affecting the loading of anticancer drug Paclitaxel on coated gold nanoparticles for breast cancer treatment |
| title_full | Evaluation of the parameters affecting the loading of anticancer drug Paclitaxel on coated gold nanoparticles for breast cancer treatment |
| title_fullStr | Evaluation of the parameters affecting the loading of anticancer drug Paclitaxel on coated gold nanoparticles for breast cancer treatment |
| title_full_unstemmed | Evaluation of the parameters affecting the loading of anticancer drug Paclitaxel on coated gold nanoparticles for breast cancer treatment |
| title_short | Evaluation of the parameters affecting the loading of anticancer drug Paclitaxel on coated gold nanoparticles for breast cancer treatment |
| title_sort | evaluation of the parameters affecting the loading of anticancer drug paclitaxel on coated gold nanoparticles for breast cancer treatment |
| topic | cancer nanomedicine nanoparticles |
| url | https://doi.org/10.1049/nbt2.12121 |
| work_keys_str_mv | AT afroozkharazmi evaluationoftheparametersaffectingtheloadingofanticancerdrugpaclitaxeloncoatedgoldnanoparticlesforbreastcancertreatment AT nedaattaran evaluationoftheparametersaffectingtheloadingofanticancerdrugpaclitaxeloncoatedgoldnanoparticlesforbreastcancertreatment |