Increased Antioxidant Performance of Lignin by Biodegradation Obtained from an Extract of the Mushroom <i>Pleurotus eryngii</i>
The aim of this study was to evaluate the antioxidant properties of the products derived from the biodegradation of lignin by the ligninolytic enzymes present in an aqueous extract of the mushroom <i>P. eryngii</i>. A mixture obtained after the incubation of lignin for 18 h with <i>...
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MDPI AG
2024-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/23/5575 |
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| author | Tania Petraglia Tiziana Latronico Antonietta Pepe Aniello Crescenzi Grazia Maria Liuzzi Rocco Rossano |
| author_facet | Tania Petraglia Tiziana Latronico Antonietta Pepe Aniello Crescenzi Grazia Maria Liuzzi Rocco Rossano |
| author_sort | Tania Petraglia |
| collection | DOAJ |
| description | The aim of this study was to evaluate the antioxidant properties of the products derived from the biodegradation of lignin by the ligninolytic enzymes present in an aqueous extract of the mushroom <i>P. eryngii</i>. A mixture obtained after the incubation of lignin for 18 h with <i>P. eryngii</i> extract was tested in vitro for its total polyphenol content, reducing power, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl (OH) radical-scavenging activities. The results showed that the enzymatic treatment of lignin enhanced its antioxidant performance. The biocompatibility of the products of lignin biodegradation and their ability to scavenge reactive oxygen species (ROS) were also tested on the astrocytic cell line DI-TNC1. The results obtained indicated that a lignin mixture incubated for 18 h does not affect cell viability or inhibit the H<sub>2</sub>O<sub>2</sub>-induced ROS production. These results suggest that the enzymatic degradation of lignin represents an efficient and ecofriendly approach to obtain lignin derivatives potentially useful for antioxidant applications. |
| format | Article |
| id | doaj-art-e6e7f597096c422aac53f11ef7827458 |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-e6e7f597096c422aac53f11ef78274582025-08-20T02:50:33ZengMDPI AGMolecules1420-30492024-11-012923557510.3390/molecules29235575Increased Antioxidant Performance of Lignin by Biodegradation Obtained from an Extract of the Mushroom <i>Pleurotus eryngii</i>Tania Petraglia0Tiziana Latronico1Antonietta Pepe2Aniello Crescenzi3Grazia Maria Liuzzi4Rocco Rossano5Department of Basic and Applied Sciences, University of Basilicata, 85100 Potenza, ItalyDepartment of Biosciences, Biotechnologies and Environment, University of Bari “Aldo Moro”, 70126 Bari, ItalyDepartment of Basic and Applied Sciences, University of Basilicata, 85100 Potenza, ItalyDepartment of Agricultural, Forestry, Food and Environmental, University of Basilicata, 85100 Potenza, ItalyDepartment of Biosciences, Biotechnologies and Environment, University of Bari “Aldo Moro”, 70126 Bari, ItalyDepartment of Basic and Applied Sciences, University of Basilicata, 85100 Potenza, ItalyThe aim of this study was to evaluate the antioxidant properties of the products derived from the biodegradation of lignin by the ligninolytic enzymes present in an aqueous extract of the mushroom <i>P. eryngii</i>. A mixture obtained after the incubation of lignin for 18 h with <i>P. eryngii</i> extract was tested in vitro for its total polyphenol content, reducing power, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl (OH) radical-scavenging activities. The results showed that the enzymatic treatment of lignin enhanced its antioxidant performance. The biocompatibility of the products of lignin biodegradation and their ability to scavenge reactive oxygen species (ROS) were also tested on the astrocytic cell line DI-TNC1. The results obtained indicated that a lignin mixture incubated for 18 h does not affect cell viability or inhibit the H<sub>2</sub>O<sub>2</sub>-induced ROS production. These results suggest that the enzymatic degradation of lignin represents an efficient and ecofriendly approach to obtain lignin derivatives potentially useful for antioxidant applications.https://www.mdpi.com/1420-3049/29/23/5575ligninantioxidantslaccasesbiodegradationROSDI-TNC1 cell line |
| spellingShingle | Tania Petraglia Tiziana Latronico Antonietta Pepe Aniello Crescenzi Grazia Maria Liuzzi Rocco Rossano Increased Antioxidant Performance of Lignin by Biodegradation Obtained from an Extract of the Mushroom <i>Pleurotus eryngii</i> Molecules lignin antioxidants laccases biodegradation ROS DI-TNC1 cell line |
| title | Increased Antioxidant Performance of Lignin by Biodegradation Obtained from an Extract of the Mushroom <i>Pleurotus eryngii</i> |
| title_full | Increased Antioxidant Performance of Lignin by Biodegradation Obtained from an Extract of the Mushroom <i>Pleurotus eryngii</i> |
| title_fullStr | Increased Antioxidant Performance of Lignin by Biodegradation Obtained from an Extract of the Mushroom <i>Pleurotus eryngii</i> |
| title_full_unstemmed | Increased Antioxidant Performance of Lignin by Biodegradation Obtained from an Extract of the Mushroom <i>Pleurotus eryngii</i> |
| title_short | Increased Antioxidant Performance of Lignin by Biodegradation Obtained from an Extract of the Mushroom <i>Pleurotus eryngii</i> |
| title_sort | increased antioxidant performance of lignin by biodegradation obtained from an extract of the mushroom i pleurotus eryngii i |
| topic | lignin antioxidants laccases biodegradation ROS DI-TNC1 cell line |
| url | https://www.mdpi.com/1420-3049/29/23/5575 |
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