Protective Effects of Oyster Peptides against Acute Alcoholic Liver Injury and Screening for Bioactive Peptides
We aimed to explore the protective effects of oyster protein-derived peptides against acute alcoholic liver disease (ALD) in mice and to screen for potential bioactive peptides with alcohol-detoxifying and liver-protecting effects. Oyster protein was extracted and hydrolyzed by two different enzymes...
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China Food Publishing Company
2025-06-01
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-11-021.pdf |
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| author | ZHANG Xiuli, CHEN Yajing, ZHENG Zhihong, CAO Wenhong, QIN Xiaoming, LIN Haisheng, CHEN Zhongqin, ZHENG Huina, ZHU Guoping, XIA Xiaoyu, GAO Jialong |
| author_facet | ZHANG Xiuli, CHEN Yajing, ZHENG Zhihong, CAO Wenhong, QIN Xiaoming, LIN Haisheng, CHEN Zhongqin, ZHENG Huina, ZHU Guoping, XIA Xiaoyu, GAO Jialong |
| author_sort | ZHANG Xiuli, CHEN Yajing, ZHENG Zhihong, CAO Wenhong, QIN Xiaoming, LIN Haisheng, CHEN Zhongqin, ZHENG Huina, ZHU Guoping, XIA Xiaoyu, GAO Jialong |
| collection | DOAJ |
| description | We aimed to explore the protective effects of oyster protein-derived peptides against acute alcoholic liver disease (ALD) in mice and to screen for potential bioactive peptides with alcohol-detoxifying and liver-protecting effects. Oyster protein was extracted and hydrolyzed by two different enzymes, neutral protease and alkaline protease, into peptides. The degree of hydrolysis, molecular mass distribution, and amino acid composition of the peptides obtained after different hydrolysis times were determined. Using an acute ALD mouse model, we evaluated the ameliorative effects of these peptides on acute ALD by measuring alanine aminotransferase (ALT), aspartate aminotransferase (AST) in the serum, and alcohol dehydrogenase (ADH), acetaldehyde dehydrogenase (ALDH), superoxide dismutase (SOD), glutathione (GSH), triglyceride (TG), and malondialdehyde (MDA) in the liver, as well as conducting pathological examination of the liver. Furthermore, peptide fractions with significant protective activity against liver injury were identified by mass spectrometry (MS) and screened. The results showed that compared with the model group, the activities of ALT and AST in the serum and the contents of MDA and TG in the liver of the peptide-treated groups was significantly decreased, the activities of SOD, GSH, ADH, and ALDH in the liver was markedly increased, and the pathological state of the liver was obviously ameliorated. Among all tested peptides, the peptides obtained after 2 and 4 h hydrolysis with alkaline protease (A-2 h) and neutral protease (N-4 h), respectively were found to be the most effective. Moreover, MS analysis identified 1 536 peptides in hydrolysate A-2 h and 1 782 peptides in hydrolysate N-4 h. After activity prediction and screening, ten oyster peptides with high activity were obtained. Molecular docking results showed that FAPPR, RFFYR, and FPGQR had strong binding affinity to ADH, indicating their potential for alcohol detoxification and liver protection. Subsequent in vitro experiments confirmed that all three peptides had an activating effect on ADH. In summary, this study provides ideas for the development of oyster-based therapeutic drugs against ALD. |
| format | Article |
| id | doaj-art-e6df6aa7ef4d445f83d94809990faaca |
| institution | DOAJ |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | China Food Publishing Company |
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| series | Shipin Kexue |
| spelling | doaj-art-e6df6aa7ef4d445f83d94809990faaca2025-08-20T02:41:11ZengChina Food Publishing CompanyShipin Kexue1002-66302025-06-01461119820710.7506/spkx1002-6630-20241111-077Protective Effects of Oyster Peptides against Acute Alcoholic Liver Injury and Screening for Bioactive PeptidesZHANG Xiuli, CHEN Yajing, ZHENG Zhihong, CAO Wenhong, QIN Xiaoming, LIN Haisheng, CHEN Zhongqin, ZHENG Huina, ZHU Guoping, XIA Xiaoyu, GAO Jialong0(1. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China;2. Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Guangdong Provincial Universities, Key Laboratory of Deep Processing of Aquatic Products, National Research and Development Branch Center for Shellfish Processing (Zhanjiang), Zhanjiang 524088, China)We aimed to explore the protective effects of oyster protein-derived peptides against acute alcoholic liver disease (ALD) in mice and to screen for potential bioactive peptides with alcohol-detoxifying and liver-protecting effects. Oyster protein was extracted and hydrolyzed by two different enzymes, neutral protease and alkaline protease, into peptides. The degree of hydrolysis, molecular mass distribution, and amino acid composition of the peptides obtained after different hydrolysis times were determined. Using an acute ALD mouse model, we evaluated the ameliorative effects of these peptides on acute ALD by measuring alanine aminotransferase (ALT), aspartate aminotransferase (AST) in the serum, and alcohol dehydrogenase (ADH), acetaldehyde dehydrogenase (ALDH), superoxide dismutase (SOD), glutathione (GSH), triglyceride (TG), and malondialdehyde (MDA) in the liver, as well as conducting pathological examination of the liver. Furthermore, peptide fractions with significant protective activity against liver injury were identified by mass spectrometry (MS) and screened. The results showed that compared with the model group, the activities of ALT and AST in the serum and the contents of MDA and TG in the liver of the peptide-treated groups was significantly decreased, the activities of SOD, GSH, ADH, and ALDH in the liver was markedly increased, and the pathological state of the liver was obviously ameliorated. Among all tested peptides, the peptides obtained after 2 and 4 h hydrolysis with alkaline protease (A-2 h) and neutral protease (N-4 h), respectively were found to be the most effective. Moreover, MS analysis identified 1 536 peptides in hydrolysate A-2 h and 1 782 peptides in hydrolysate N-4 h. After activity prediction and screening, ten oyster peptides with high activity were obtained. Molecular docking results showed that FAPPR, RFFYR, and FPGQR had strong binding affinity to ADH, indicating their potential for alcohol detoxification and liver protection. Subsequent in vitro experiments confirmed that all three peptides had an activating effect on ADH. In summary, this study provides ideas for the development of oyster-based therapeutic drugs against ALD.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-11-021.pdfoyster; acute alcoholic liver disease; bioactive peptides; alcohol dehydrogenase; molecular docking |
| spellingShingle | ZHANG Xiuli, CHEN Yajing, ZHENG Zhihong, CAO Wenhong, QIN Xiaoming, LIN Haisheng, CHEN Zhongqin, ZHENG Huina, ZHU Guoping, XIA Xiaoyu, GAO Jialong Protective Effects of Oyster Peptides against Acute Alcoholic Liver Injury and Screening for Bioactive Peptides Shipin Kexue oyster; acute alcoholic liver disease; bioactive peptides; alcohol dehydrogenase; molecular docking |
| title | Protective Effects of Oyster Peptides against Acute Alcoholic Liver Injury and Screening for Bioactive Peptides |
| title_full | Protective Effects of Oyster Peptides against Acute Alcoholic Liver Injury and Screening for Bioactive Peptides |
| title_fullStr | Protective Effects of Oyster Peptides against Acute Alcoholic Liver Injury and Screening for Bioactive Peptides |
| title_full_unstemmed | Protective Effects of Oyster Peptides against Acute Alcoholic Liver Injury and Screening for Bioactive Peptides |
| title_short | Protective Effects of Oyster Peptides against Acute Alcoholic Liver Injury and Screening for Bioactive Peptides |
| title_sort | protective effects of oyster peptides against acute alcoholic liver injury and screening for bioactive peptides |
| topic | oyster; acute alcoholic liver disease; bioactive peptides; alcohol dehydrogenase; molecular docking |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-11-021.pdf |
| work_keys_str_mv | AT zhangxiulichenyajingzhengzhihongcaowenhongqinxiaominglinhaishengchenzhongqinzhenghuinazhuguopingxiaxiaoyugaojialong protectiveeffectsofoysterpeptidesagainstacutealcoholicliverinjuryandscreeningforbioactivepeptides |