Single-nucleus profiling of the left ventricle of the mouse heart after chronic stress
Abstract It is widely accepted that stress and cardiac disease are related, but the exact mechanism is still up for debate. Although stress has been scientifically confirmed as a causative factor, there is still a lack of qualitative and quantitative indicator systems for stress-induced cardiac inju...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-05556-x |
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| author | Yaping Li Xia Liu Huaxing Zhang Qian Wang Yan Zhu Haibo Xu Qinmin Chen Shulin Xiang Han Xiao Qian Qi Bin Cong |
| author_facet | Yaping Li Xia Liu Huaxing Zhang Qian Wang Yan Zhu Haibo Xu Qinmin Chen Shulin Xiang Han Xiao Qian Qi Bin Cong |
| author_sort | Yaping Li |
| collection | DOAJ |
| description | Abstract It is widely accepted that stress and cardiac disease are related, but the exact mechanism is still up for debate. Although stress has been scientifically confirmed as a causative factor, there is still a lack of qualitative and quantitative indicator systems for stress-induced cardiac injury. The forensic evidence frequently indicates that cases of sudden cardiac death are often preceded by prolonged exposure to chronic stress factors. However, the cellular responses and mechanisms that trigger and regulate these activities in the pathological and physiological processes of chronic stress are still poorly understood. The left ventricle (LV), as a critical target organ in cardiovascular diseases, still has unclear cellular heterogeneity under chronic stress. Using single-nucleus RNA sequencing (snRNA-seq), we established a cellular atlas of 99,154 LV cells (42,491 stress and 56,663 control), comprehensively profiling all major cardiac cell types. The resulting dataset not only provides a foundation for deciphering the molecular mechanisms underlying chronic stress in cardiovascular dysfunction but also enables the identification of potential biomarkers for future diagnostic and therapeutic strategies. |
| format | Article |
| id | doaj-art-e6dddbd2d6634892a49e1321344bb4b9 |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-e6dddbd2d6634892a49e1321344bb4b92025-08-20T03:42:30ZengNature PortfolioScientific Data2052-44632025-07-011211810.1038/s41597-025-05556-xSingle-nucleus profiling of the left ventricle of the mouse heart after chronic stressYaping Li0Xia Liu1Huaxing Zhang2Qian Wang3Yan Zhu4Haibo Xu5Qinmin Chen6Shulin Xiang7Han Xiao8Qian Qi9Bin Cong10College of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identifications, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, Chinese Academy of Medical SciencesCollege of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identifications, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, Chinese Academy of Medical SciencesCore Facilities and Centers, Hebei Medical UniversityCollege of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identifications, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, Chinese Academy of Medical SciencesCore Facilities and Centers, Hebei Medical UniversityThe First Department of Pulmonary and Critical Care Medicine, The Second Hospital of Hebei Medical University, Hebei Key Laboratory of Respiratory Critical Care, Hebei Institute of Respiratory DiseasesCollege of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identifications, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, Chinese Academy of Medical SciencesCollege of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identifications, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, Chinese Academy of Medical SciencesCollege of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identifications, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, Chinese Academy of Medical SciencesCollege of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identifications, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, Chinese Academy of Medical SciencesCollege of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identifications, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, Chinese Academy of Medical SciencesAbstract It is widely accepted that stress and cardiac disease are related, but the exact mechanism is still up for debate. Although stress has been scientifically confirmed as a causative factor, there is still a lack of qualitative and quantitative indicator systems for stress-induced cardiac injury. The forensic evidence frequently indicates that cases of sudden cardiac death are often preceded by prolonged exposure to chronic stress factors. However, the cellular responses and mechanisms that trigger and regulate these activities in the pathological and physiological processes of chronic stress are still poorly understood. The left ventricle (LV), as a critical target organ in cardiovascular diseases, still has unclear cellular heterogeneity under chronic stress. Using single-nucleus RNA sequencing (snRNA-seq), we established a cellular atlas of 99,154 LV cells (42,491 stress and 56,663 control), comprehensively profiling all major cardiac cell types. The resulting dataset not only provides a foundation for deciphering the molecular mechanisms underlying chronic stress in cardiovascular dysfunction but also enables the identification of potential biomarkers for future diagnostic and therapeutic strategies.https://doi.org/10.1038/s41597-025-05556-x |
| spellingShingle | Yaping Li Xia Liu Huaxing Zhang Qian Wang Yan Zhu Haibo Xu Qinmin Chen Shulin Xiang Han Xiao Qian Qi Bin Cong Single-nucleus profiling of the left ventricle of the mouse heart after chronic stress Scientific Data |
| title | Single-nucleus profiling of the left ventricle of the mouse heart after chronic stress |
| title_full | Single-nucleus profiling of the left ventricle of the mouse heart after chronic stress |
| title_fullStr | Single-nucleus profiling of the left ventricle of the mouse heart after chronic stress |
| title_full_unstemmed | Single-nucleus profiling of the left ventricle of the mouse heart after chronic stress |
| title_short | Single-nucleus profiling of the left ventricle of the mouse heart after chronic stress |
| title_sort | single nucleus profiling of the left ventricle of the mouse heart after chronic stress |
| url | https://doi.org/10.1038/s41597-025-05556-x |
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