Mesenchymal stem cells from periapical lesions modulate cytokine production by local immune cells
Background/Aim. Mesenchymal stem cells (MSCs) have been shown to suppress immune and inflammatory reactions. However, it is not known whether MSCs from inflammatory tissues, such as periapical lesions (PLs) have similar effects. This question was addressed in this study in which the aim was to exami...
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| Format: | Article |
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2018-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2018/0042-84501600272M.pdf |
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| author | Marković Milan Tomić Sergej Đokić Jelena Mihajlović Dušan Vučević Dragana Gazivoda Dragan Duka Miloš Čolić Miodrag |
| author_facet | Marković Milan Tomić Sergej Đokić Jelena Mihajlović Dušan Vučević Dragana Gazivoda Dragan Duka Miloš Čolić Miodrag |
| author_sort | Marković Milan |
| collection | DOAJ |
| description | Background/Aim. Mesenchymal stem cells (MSCs) have been shown to suppress immune and inflammatory reactions. However, it is not known whether MSCs from inflammatory tissues, such as periapical lesions (PLs) have similar effects. This question was addressed in this study in which the aim was to examine the capacity of PL-MSCs for modulating cytokine production by local immune cells. Methods. PL-MSCs were isolated from asymptomatic (as) and symptomatic (sy) PLs. Their phenotype was analyzed by flow cytometry by detecting MSC surface markers. Anti-inflammatory and immunomodulatory properties of PL-MSCs were examined by measuring cytokine production in direct co-culture experiments with mononuclear cells (MNCs) isolated from asPLs and syPLs, respectively. The levels of cytokines in supernatants were determined by specific ELISA kits. Results. Both PL-MSCs lines were characterized by typical MSC phenotype, with the predominance of CD29, CD44, CD90, CD105 and CD166. However, the lines, independently of their similar phenotype had the same modulatory effect on cytokine production, but the response of asPL-MNCs and syPL-MNCs was different, in spite of similar composition of these MNCs. Both MSC lines inhibited the production of inflammatory cytokines, such as interleukin-1_ (IL-1_) and tumor necrosis factor-_ (TNF-_). However, IL-8 was only down-regulated in the co-culture of these MSC lines with syPL-MNCs. The PL-MSCs also modulated the production of immunoregulatory cytokines. Transforming growth factor-_ (TGF-_) was up-regulated by both as- and syPL-MNCs but IL-10 was up-regulated only by asPL-MNCs. Conclusion. Our results showed that PL-MSCs contribute to the restriction of local inflammatory and immune responses, but this effect is probably less efficient during the exacerbation of PL inflammation. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 175102] |
| format | Article |
| id | doaj-art-e6d978290604418bbcfe9f8fea11d6e2 |
| institution | OA Journals |
| issn | 0042-8450 2406-0720 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-e6d978290604418bbcfe9f8fea11d6e22025-08-20T02:04:11ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502406-07202018-01-0175547348010.2298/VSP160901272M0042-84501600272MMesenchymal stem cells from periapical lesions modulate cytokine production by local immune cellsMarković Milan0Tomić Sergej1Đokić Jelena2Mihajlović Dušan3Vučević Dragana4Gazivoda Dragan5Duka Miloš6Čolić Miodrag7Military Medical Academy, Institute for Medical Research, Clinic for Stomatology, Belgrade + Faculty of Medicine, NišUniversity of Defence in Belgrade, Medical Faculty of the Military Medical Academy, BelgradeMilitary Medical Academy, Institute for Medical Research, Clinic for Stomatology, Belgrade University of Defence in Belgrade, Medical Faculty of the Military Medical Academy, BelgradeMilitary Medical Academy, Institute for Medical Research, Clinic for Stomatology, Belgrade + University of Defence in Belgrade, Medical Faculty of the Military Medical Academy, BelgradeMilitary Medical Academy, Department for Oral Surgery, BelgradeMilitary Medical Academy, Department for Oral Surgery, BelgradeUniversity of Defence in Belgrade, Medical Faculty of the Military Medical Academy, Belgrade + INEP – Institute for Nuclear Energy, BelgradeBackground/Aim. Mesenchymal stem cells (MSCs) have been shown to suppress immune and inflammatory reactions. However, it is not known whether MSCs from inflammatory tissues, such as periapical lesions (PLs) have similar effects. This question was addressed in this study in which the aim was to examine the capacity of PL-MSCs for modulating cytokine production by local immune cells. Methods. PL-MSCs were isolated from asymptomatic (as) and symptomatic (sy) PLs. Their phenotype was analyzed by flow cytometry by detecting MSC surface markers. Anti-inflammatory and immunomodulatory properties of PL-MSCs were examined by measuring cytokine production in direct co-culture experiments with mononuclear cells (MNCs) isolated from asPLs and syPLs, respectively. The levels of cytokines in supernatants were determined by specific ELISA kits. Results. Both PL-MSCs lines were characterized by typical MSC phenotype, with the predominance of CD29, CD44, CD90, CD105 and CD166. However, the lines, independently of their similar phenotype had the same modulatory effect on cytokine production, but the response of asPL-MNCs and syPL-MNCs was different, in spite of similar composition of these MNCs. Both MSC lines inhibited the production of inflammatory cytokines, such as interleukin-1_ (IL-1_) and tumor necrosis factor-_ (TNF-_). However, IL-8 was only down-regulated in the co-culture of these MSC lines with syPL-MNCs. The PL-MSCs also modulated the production of immunoregulatory cytokines. Transforming growth factor-_ (TGF-_) was up-regulated by both as- and syPL-MNCs but IL-10 was up-regulated only by asPL-MNCs. Conclusion. Our results showed that PL-MSCs contribute to the restriction of local inflammatory and immune responses, but this effect is probably less efficient during the exacerbation of PL inflammation. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 175102]http://www.doiserbia.nb.rs/img/doi/0042-8450/2018/0042-84501600272M.pdfperiapical diseasesstem cellsperiapical abscessinflammationcytokinesphenotypeflow cytometry |
| spellingShingle | Marković Milan Tomić Sergej Đokić Jelena Mihajlović Dušan Vučević Dragana Gazivoda Dragan Duka Miloš Čolić Miodrag Mesenchymal stem cells from periapical lesions modulate cytokine production by local immune cells Vojnosanitetski Pregled periapical diseases stem cells periapical abscess inflammation cytokines phenotype flow cytometry |
| title | Mesenchymal stem cells from periapical lesions modulate cytokine production by local immune cells |
| title_full | Mesenchymal stem cells from periapical lesions modulate cytokine production by local immune cells |
| title_fullStr | Mesenchymal stem cells from periapical lesions modulate cytokine production by local immune cells |
| title_full_unstemmed | Mesenchymal stem cells from periapical lesions modulate cytokine production by local immune cells |
| title_short | Mesenchymal stem cells from periapical lesions modulate cytokine production by local immune cells |
| title_sort | mesenchymal stem cells from periapical lesions modulate cytokine production by local immune cells |
| topic | periapical diseases stem cells periapical abscess inflammation cytokines phenotype flow cytometry |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2018/0042-84501600272M.pdf |
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