Characterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicing
In trypanosomatids, gene expression is mainly regulated at posttranscriptional level, through mechanisms based on the interaction between RNA Binding Proteins [RBPs] and motifs present in the untranslated regions [UTRs] of them RNAs, which altogether form ribonucleoproteic complexes [RNP] that defin...
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Pontificia Universidad Javeriana
2018-09-01
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| Series: | Universitas Scientiarum |
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| Online Access: | https://revistas.javeriana.edu.co/index.php/scientarium/article/view/21288 |
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| author | César Augusto Ramírez Julián Camilo Casas María Isabel Ospina José María Requena Concepción J. Puerta |
| author_facet | César Augusto Ramírez Julián Camilo Casas María Isabel Ospina José María Requena Concepción J. Puerta |
| author_sort | César Augusto Ramírez |
| collection | DOAJ |
| description | In trypanosomatids, gene expression is mainly regulated at posttranscriptional level, through mechanisms based on the interaction between RNA Binding Proteins [RBPs] and motifs present in the untranslated regions [UTRs] of them RNAs, which altogether form ribonucleoproteic complexes [RNP] that define the fate of the mRNA. The pre-mRNA derived from the LYT1 gene of Trypanosoma cruzi, is processed by alternative trans-splicing, resulting in different mRNAs which code for the isoforms mLYT1 and kLYT1, proteins having differential expression, cellular location and function. The aim of this study was to characterize the 5’ and 3’ UTRs of the LYT1 mRNAs as the initial step towards the objective of identification of the RBPs responsible for their differential expression. The presence of the two types of 5’ UTRs were confirmed in two T. cruzi isolates belonging to the DTU I, thus, corroborating the occurrence of alternative trans-splicing also in the LYT1 gene of this T.cruzi DTU. In addition, for the first time, was unscovered the existence of two types of LYT1 mRNAs transcripts, differing in length by 116 nts, that are generated by alternative polyadenylation. Furthermore, an in-silico analysis of the experimentally obtained UTRs, and ten additional LYT1 sequences retrieved from TritrypDB and GenBank databases, together with a thoroughly search of structural motifs, showed a remarkable conservation of relevant structural motifs previously associated with RNA metabolism in the different UTRs; these elements might be involved in the differential stage-specific expression of each LYT1 isoform. |
| format | Article |
| id | doaj-art-e6d6af8dd14d483abb6939d774822164 |
| institution | DOAJ |
| issn | 0122-7483 2027-1352 |
| language | English |
| publishDate | 2018-09-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj-art-e6d6af8dd14d483abb6939d7748221642025-08-20T03:09:41ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522018-09-0123226729010.11144/Javeriana.SC23-2.cotmCharacterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicingCésar Augusto Ramírez0Julián Camilo Casas1María Isabel Ospina2José María Requena3Concepción J. Puerta4Laboratorio de Parasitología Molecular. Departamento de Microbiología. Facultad de Ciencias. Pontificia Universidad JaverianaLaboratorio de Parasitología Molecular. Departamento de Microbiología. Facultad de Ciencias. Pontificia Universidad JaverianaLaboratorio de Parasitología Molecular. Departamento de Microbiología. Facultad de Ciencias. Pontificia Universidad JaverianaCentro de Biología Molecular Severo Ochoa, Universidad Autónomade Madrid, Madrid, EspañaLaboratorio de Parasitología Molecular. Departamento de Microbiología. Facultad de Ciencias. Pontificia Universidad JaverianaIn trypanosomatids, gene expression is mainly regulated at posttranscriptional level, through mechanisms based on the interaction between RNA Binding Proteins [RBPs] and motifs present in the untranslated regions [UTRs] of them RNAs, which altogether form ribonucleoproteic complexes [RNP] that define the fate of the mRNA. The pre-mRNA derived from the LYT1 gene of Trypanosoma cruzi, is processed by alternative trans-splicing, resulting in different mRNAs which code for the isoforms mLYT1 and kLYT1, proteins having differential expression, cellular location and function. The aim of this study was to characterize the 5’ and 3’ UTRs of the LYT1 mRNAs as the initial step towards the objective of identification of the RBPs responsible for their differential expression. The presence of the two types of 5’ UTRs were confirmed in two T. cruzi isolates belonging to the DTU I, thus, corroborating the occurrence of alternative trans-splicing also in the LYT1 gene of this T.cruzi DTU. In addition, for the first time, was unscovered the existence of two types of LYT1 mRNAs transcripts, differing in length by 116 nts, that are generated by alternative polyadenylation. Furthermore, an in-silico analysis of the experimentally obtained UTRs, and ten additional LYT1 sequences retrieved from TritrypDB and GenBank databases, together with a thoroughly search of structural motifs, showed a remarkable conservation of relevant structural motifs previously associated with RNA metabolism in the different UTRs; these elements might be involved in the differential stage-specific expression of each LYT1 isoform.https://revistas.javeriana.edu.co/index.php/scientarium/article/view/21288Trypanosoma cruziUntranslated region [UTR]RNA binding proteins [RBP]Regulation of gene expressionLYT1 gene |
| spellingShingle | César Augusto Ramírez Julián Camilo Casas María Isabel Ospina José María Requena Concepción J. Puerta Characterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicing Universitas Scientiarum Trypanosoma cruzi Untranslated region [UTR] RNA binding proteins [RBP] Regulation of gene expression LYT1 gene |
| title | Characterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicing |
| title_full | Characterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicing |
| title_fullStr | Characterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicing |
| title_full_unstemmed | Characterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicing |
| title_short | Characterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicing |
| title_sort | characterization of the mrna untranslated regions utr of the trypanosoma cruzi lyt1 isoforms derived by alternative trans splicing |
| topic | Trypanosoma cruzi Untranslated region [UTR] RNA binding proteins [RBP] Regulation of gene expression LYT1 gene |
| url | https://revistas.javeriana.edu.co/index.php/scientarium/article/view/21288 |
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