Vaginal Lactoferrin Modulates PGE2, MMP-9, MMP-2, and TIMP-1 Amniotic Fluid Concentrations
Inflammation plays an important role in pregnancy, and cytokine and matrix metalloproteases (MMPs) imbalance has been associated with premature rupture of membranes and increased risk of preterm delivery. Previous studies have demonstrated that lactoferrin (LF), an iron-binding protein with anti-inf...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/3648719 |
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| author | Alessandro Trentini Martina Maritati Carlo Cervellati Maria C. Manfrinato Arianna Gonelli Carlo A. Volta Fortunato Vesce Pantaleo Greco Franco Dallocchio Tiziana Bellini Carlo Contini |
| author_facet | Alessandro Trentini Martina Maritati Carlo Cervellati Maria C. Manfrinato Arianna Gonelli Carlo A. Volta Fortunato Vesce Pantaleo Greco Franco Dallocchio Tiziana Bellini Carlo Contini |
| author_sort | Alessandro Trentini |
| collection | DOAJ |
| description | Inflammation plays an important role in pregnancy, and cytokine and matrix metalloproteases (MMPs) imbalance has been associated with premature rupture of membranes and increased risk of preterm delivery. Previous studies have demonstrated that lactoferrin (LF), an iron-binding protein with anti-inflammatory properties, is able to decrease amniotic fluid (AF) levels of IL-6. Therefore, we aimed to evaluate the effect of vaginal LF administration on amniotic fluid PGE2 level and MMP-TIMP system in women undergoing genetic amniocentesis. One hundred and eleven women were randomly divided into controls (n=57) or treated with LF 4 hours before amniocentesis (n=54). Amniotic fluid PGE2, active MMP-9 and MMP-2, and TIMP-1 and TIMP-2 concentrations were determined by commercially available assays and the values were normalized by AF creatinine concentration. PGE2, active MMP-9, and its inhibitor TIMP-1 were lower in LF-treated group than in controls (p<0.01, p<0.005, and p<0.001, resp.). Conversely, active MMP-2 (p<0.0001) and MMP-2/TIMP-2 molar ratio (p<0.001) were increased, whilst TIMP-2 was unchanged. Our data suggest that LF administration is able to modulate the inflammatory response following amniocentesis, which may counteract cytokine and prostanoid imbalance that leads to abortion. This trial is registered with Clinical Trial number NCT02695563. |
| format | Article |
| id | doaj-art-e6ca860a22fb4efab0fac2878684380b |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-e6ca860a22fb4efab0fac2878684380b2025-08-20T02:21:10ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/36487193648719Vaginal Lactoferrin Modulates PGE2, MMP-9, MMP-2, and TIMP-1 Amniotic Fluid ConcentrationsAlessandro Trentini0Martina Maritati1Carlo Cervellati2Maria C. Manfrinato3Arianna Gonelli4Carlo A. Volta5Fortunato Vesce6Pantaleo Greco7Franco Dallocchio8Tiziana Bellini9Carlo Contini10Section of Medical Biochemistry, Molecular Biology and Genetics, Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, 44121 Ferrara, ItalySection of Dermatology and Infectious Diseases, Department of Medical Sciences, University of Ferrara, 44121 Ferrara, ItalySection of Medical Biochemistry, Molecular Biology and Genetics, Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, 44121 Ferrara, ItalySection of Medical Biochemistry, Molecular Biology and Genetics, Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, 44121 Ferrara, ItalyDepartment of Morphology, Surgery and Experimental Medicine, University of Ferrara, 44121 Ferrara, ItalySection of Orthopedics, Obstetrics and Gynecology and Anesthesia and Resuscitation, Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, 44121 Ferrara, ItalySection of Dermatology and Infectious Diseases, Department of Medical Sciences, University of Ferrara, 44121 Ferrara, ItalySection of Orthopedics, Obstetrics and Gynecology and Anesthesia and Resuscitation, Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, 44121 Ferrara, ItalySection of Medical Biochemistry, Molecular Biology and Genetics, Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, 44121 Ferrara, ItalySection of Medical Biochemistry, Molecular Biology and Genetics, Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, 44121 Ferrara, ItalySection of Dermatology and Infectious Diseases, Department of Medical Sciences, University of Ferrara, 44121 Ferrara, ItalyInflammation plays an important role in pregnancy, and cytokine and matrix metalloproteases (MMPs) imbalance has been associated with premature rupture of membranes and increased risk of preterm delivery. Previous studies have demonstrated that lactoferrin (LF), an iron-binding protein with anti-inflammatory properties, is able to decrease amniotic fluid (AF) levels of IL-6. Therefore, we aimed to evaluate the effect of vaginal LF administration on amniotic fluid PGE2 level and MMP-TIMP system in women undergoing genetic amniocentesis. One hundred and eleven women were randomly divided into controls (n=57) or treated with LF 4 hours before amniocentesis (n=54). Amniotic fluid PGE2, active MMP-9 and MMP-2, and TIMP-1 and TIMP-2 concentrations were determined by commercially available assays and the values were normalized by AF creatinine concentration. PGE2, active MMP-9, and its inhibitor TIMP-1 were lower in LF-treated group than in controls (p<0.01, p<0.005, and p<0.001, resp.). Conversely, active MMP-2 (p<0.0001) and MMP-2/TIMP-2 molar ratio (p<0.001) were increased, whilst TIMP-2 was unchanged. Our data suggest that LF administration is able to modulate the inflammatory response following amniocentesis, which may counteract cytokine and prostanoid imbalance that leads to abortion. This trial is registered with Clinical Trial number NCT02695563.http://dx.doi.org/10.1155/2016/3648719 |
| spellingShingle | Alessandro Trentini Martina Maritati Carlo Cervellati Maria C. Manfrinato Arianna Gonelli Carlo A. Volta Fortunato Vesce Pantaleo Greco Franco Dallocchio Tiziana Bellini Carlo Contini Vaginal Lactoferrin Modulates PGE2, MMP-9, MMP-2, and TIMP-1 Amniotic Fluid Concentrations Mediators of Inflammation |
| title | Vaginal Lactoferrin Modulates PGE2, MMP-9, MMP-2, and TIMP-1 Amniotic Fluid Concentrations |
| title_full | Vaginal Lactoferrin Modulates PGE2, MMP-9, MMP-2, and TIMP-1 Amniotic Fluid Concentrations |
| title_fullStr | Vaginal Lactoferrin Modulates PGE2, MMP-9, MMP-2, and TIMP-1 Amniotic Fluid Concentrations |
| title_full_unstemmed | Vaginal Lactoferrin Modulates PGE2, MMP-9, MMP-2, and TIMP-1 Amniotic Fluid Concentrations |
| title_short | Vaginal Lactoferrin Modulates PGE2, MMP-9, MMP-2, and TIMP-1 Amniotic Fluid Concentrations |
| title_sort | vaginal lactoferrin modulates pge2 mmp 9 mmp 2 and timp 1 amniotic fluid concentrations |
| url | http://dx.doi.org/10.1155/2016/3648719 |
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