Landscape and regulation of mRNA translation in the early C. elegans embryo
Summary: Animal embryos rely on regulated translation of maternally deposited mRNAs to drive early development. Using low-input ribosome profiling combined with RNA sequencing on precisely staged embryos, we measure mRNA translation during the first four cell cycles of C. elegans development. We unc...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124725005492 |
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| author | Yash Shukla Vighnesh Ghatpande Cindy F. Hu Daniel J. Dickinson Can Cenik |
| author_facet | Yash Shukla Vighnesh Ghatpande Cindy F. Hu Daniel J. Dickinson Can Cenik |
| author_sort | Yash Shukla |
| collection | DOAJ |
| description | Summary: Animal embryos rely on regulated translation of maternally deposited mRNAs to drive early development. Using low-input ribosome profiling combined with RNA sequencing on precisely staged embryos, we measure mRNA translation during the first four cell cycles of C. elegans development. We uncover stage-specific patterns of developmentally coordinated translational regulation. Our results confirm that mRNA localization correlates with translational efficiency, though initial translational repression in germline precursors occurs before P-granule association. Our analysis suggests that the RNA-binding protein OMA-1 represses the translation of its target mRNAs in a stage-specific manner while indirectly promoting the translational efficiency of other transcripts. These findings illuminate how post-transcriptional mechanisms shape the embryonic proteome to direct cell differentiation, with implications for understanding similar regulation across species where maternal factors guide early development. |
| format | Article |
| id | doaj-art-e6c6af22470c4247845d4e8f9b1e478b |
| institution | Kabale University |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-e6c6af22470c4247845d4e8f9b1e478b2025-08-20T03:55:22ZengElsevierCell Reports2211-12472025-06-0144611577810.1016/j.celrep.2025.115778Landscape and regulation of mRNA translation in the early C. elegans embryoYash Shukla0Vighnesh Ghatpande1Cindy F. Hu2Daniel J. Dickinson3Can Cenik4Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USADepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USADepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USADepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA; Corresponding authorDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA; Corresponding authorSummary: Animal embryos rely on regulated translation of maternally deposited mRNAs to drive early development. Using low-input ribosome profiling combined with RNA sequencing on precisely staged embryos, we measure mRNA translation during the first four cell cycles of C. elegans development. We uncover stage-specific patterns of developmentally coordinated translational regulation. Our results confirm that mRNA localization correlates with translational efficiency, though initial translational repression in germline precursors occurs before P-granule association. Our analysis suggests that the RNA-binding protein OMA-1 represses the translation of its target mRNAs in a stage-specific manner while indirectly promoting the translational efficiency of other transcripts. These findings illuminate how post-transcriptional mechanisms shape the embryonic proteome to direct cell differentiation, with implications for understanding similar regulation across species where maternal factors guide early development.http://www.sciencedirect.com/science/article/pii/S2211124725005492CP: Molecular biologyCP: Developmental biology |
| spellingShingle | Yash Shukla Vighnesh Ghatpande Cindy F. Hu Daniel J. Dickinson Can Cenik Landscape and regulation of mRNA translation in the early C. elegans embryo Cell Reports CP: Molecular biology CP: Developmental biology |
| title | Landscape and regulation of mRNA translation in the early C. elegans embryo |
| title_full | Landscape and regulation of mRNA translation in the early C. elegans embryo |
| title_fullStr | Landscape and regulation of mRNA translation in the early C. elegans embryo |
| title_full_unstemmed | Landscape and regulation of mRNA translation in the early C. elegans embryo |
| title_short | Landscape and regulation of mRNA translation in the early C. elegans embryo |
| title_sort | landscape and regulation of mrna translation in the early c elegans embryo |
| topic | CP: Molecular biology CP: Developmental biology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124725005492 |
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