Health-Promoting Properties of Pectin–Polyphenol Complex Extracted from Olive Oil By-Product Alperujo: Antioxidant, Antiproliferative, and Anti-Inflammatory Activities
This research explores the health-promoting properties of the pectin–polyphenol complex extracted from alperujo, a by-product of olive oil production. This study investigates the chemical composition and antioxidant activity of the extracts, revealing their high antioxidant activity in vitro. Cell v...
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| Format: | Article |
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MDPI AG
2024-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/13/9/1066 |
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| author | Alejandra Bermúdez-Oria María Luisa Castejón Fátima Rubio-Senent Guillermo Rodríguez-Gutiérrez Juan Fernández-Bolaños |
| author_facet | Alejandra Bermúdez-Oria María Luisa Castejón Fátima Rubio-Senent Guillermo Rodríguez-Gutiérrez Juan Fernández-Bolaños |
| author_sort | Alejandra Bermúdez-Oria |
| collection | DOAJ |
| description | This research explores the health-promoting properties of the pectin–polyphenol complex extracted from alperujo, a by-product of olive oil production. This study investigates the chemical composition and antioxidant activity of the extracts, revealing their high antioxidant activity in vitro. Cell viability assays conducted on colon carcinoma cells (Caco-2) demonstrate the inhibitory effect of the extracts on cell proliferation. However, the extracts do not affect the viability of differentiated Caco-2 cells, suggesting a selective antiproliferative action. Additionally, the extracts reduce intracellular reactive oxygen species (ROS) and nitrite (NO) production in LPS-stimulated murine peritoneal macrophages. Furthermore, the extracts exhibit anti-inflammatory effects by downregulating the secretion of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 in these macrophages. These findings highlight the potential of pectin–polyphenol complexes as functional ingredients with significant health benefits, demonstrating antioxidant, antiproliferative, and anti-inflammatory properties. |
| format | Article |
| id | doaj-art-e6c0f686d198406d98ee3652e7f13f74 |
| institution | OA Journals |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-e6c0f686d198406d98ee3652e7f13f742025-08-20T01:55:57ZengMDPI AGAntioxidants2076-39212024-08-01139106610.3390/antiox13091066Health-Promoting Properties of Pectin–Polyphenol Complex Extracted from Olive Oil By-Product Alperujo: Antioxidant, Antiproliferative, and Anti-Inflammatory ActivitiesAlejandra Bermúdez-Oria0María Luisa Castejón1Fátima Rubio-Senent2Guillermo Rodríguez-Gutiérrez3Juan Fernández-Bolaños4Department of Food Phytochemistry, Instituto de la Grasa (Spanish National Research Council, CSIC), Pablo de Olavide University Campus, Building 46, Ctra. de Utrera km. 1, 41013 Seville, SpainDepartment of Food Phytochemistry, Instituto de la Grasa (Spanish National Research Council, CSIC), Pablo de Olavide University Campus, Building 46, Ctra. de Utrera km. 1, 41013 Seville, SpainDepartment of Food Phytochemistry, Instituto de la Grasa (Spanish National Research Council, CSIC), Pablo de Olavide University Campus, Building 46, Ctra. de Utrera km. 1, 41013 Seville, SpainDepartment of Food Phytochemistry, Instituto de la Grasa (Spanish National Research Council, CSIC), Pablo de Olavide University Campus, Building 46, Ctra. de Utrera km. 1, 41013 Seville, SpainDepartment of Food Phytochemistry, Instituto de la Grasa (Spanish National Research Council, CSIC), Pablo de Olavide University Campus, Building 46, Ctra. de Utrera km. 1, 41013 Seville, SpainThis research explores the health-promoting properties of the pectin–polyphenol complex extracted from alperujo, a by-product of olive oil production. This study investigates the chemical composition and antioxidant activity of the extracts, revealing their high antioxidant activity in vitro. Cell viability assays conducted on colon carcinoma cells (Caco-2) demonstrate the inhibitory effect of the extracts on cell proliferation. However, the extracts do not affect the viability of differentiated Caco-2 cells, suggesting a selective antiproliferative action. Additionally, the extracts reduce intracellular reactive oxygen species (ROS) and nitrite (NO) production in LPS-stimulated murine peritoneal macrophages. Furthermore, the extracts exhibit anti-inflammatory effects by downregulating the secretion of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 in these macrophages. These findings highlight the potential of pectin–polyphenol complexes as functional ingredients with significant health benefits, demonstrating antioxidant, antiproliferative, and anti-inflammatory properties.https://www.mdpi.com/2076-3921/13/9/1066olive oil by-productsalperujopectin–polyphenol complexantioxidant activityantiproliferative effectsinflammation |
| spellingShingle | Alejandra Bermúdez-Oria María Luisa Castejón Fátima Rubio-Senent Guillermo Rodríguez-Gutiérrez Juan Fernández-Bolaños Health-Promoting Properties of Pectin–Polyphenol Complex Extracted from Olive Oil By-Product Alperujo: Antioxidant, Antiproliferative, and Anti-Inflammatory Activities Antioxidants olive oil by-products alperujo pectin–polyphenol complex antioxidant activity antiproliferative effects inflammation |
| title | Health-Promoting Properties of Pectin–Polyphenol Complex Extracted from Olive Oil By-Product Alperujo: Antioxidant, Antiproliferative, and Anti-Inflammatory Activities |
| title_full | Health-Promoting Properties of Pectin–Polyphenol Complex Extracted from Olive Oil By-Product Alperujo: Antioxidant, Antiproliferative, and Anti-Inflammatory Activities |
| title_fullStr | Health-Promoting Properties of Pectin–Polyphenol Complex Extracted from Olive Oil By-Product Alperujo: Antioxidant, Antiproliferative, and Anti-Inflammatory Activities |
| title_full_unstemmed | Health-Promoting Properties of Pectin–Polyphenol Complex Extracted from Olive Oil By-Product Alperujo: Antioxidant, Antiproliferative, and Anti-Inflammatory Activities |
| title_short | Health-Promoting Properties of Pectin–Polyphenol Complex Extracted from Olive Oil By-Product Alperujo: Antioxidant, Antiproliferative, and Anti-Inflammatory Activities |
| title_sort | health promoting properties of pectin polyphenol complex extracted from olive oil by product alperujo antioxidant antiproliferative and anti inflammatory activities |
| topic | olive oil by-products alperujo pectin–polyphenol complex antioxidant activity antiproliferative effects inflammation |
| url | https://www.mdpi.com/2076-3921/13/9/1066 |
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