Anti bacterial efficacy of photo catalytic active titanium di oxide (TiO2) nanoparticles synthesized via green science principles against food spoilage pathogenic bacteria

Green synthesis of nanoparticles is becoming one of the robust techniques, which may be suitable alternatives for chemical and physical methods. In the present research work, anti bacterial activity of green synthesized titanium di oxide (TiO2) nanoaparticles was screened against bacterial strains a...

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Main Authors: L.F.A. Anand Raj, A. Annushrie, S. Karthick Raja Namasivayam
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:The Microbe
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Online Access:http://www.sciencedirect.com/science/article/pii/S2950194625000998
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author L.F.A. Anand Raj
A. Annushrie
S. Karthick Raja Namasivayam
author_facet L.F.A. Anand Raj
A. Annushrie
S. Karthick Raja Namasivayam
author_sort L.F.A. Anand Raj
collection DOAJ
description Green synthesis of nanoparticles is becoming one of the robust techniques, which may be suitable alternatives for chemical and physical methods. In the present research work, anti bacterial activity of green synthesized titanium di oxide (TiO2) nanoaparticles was screened against bacterial strains against food spoilage pathogenic bacterial strains Escherichia coli and Bacillus subtilis isolated from spoiled canned foods was studied followed by investigating anti oxidant and photocatalytic activity. Nanoparticles were fabricated from Talinum fruticosum leaf extract as the reducing agent. The green synthesized TiO2 NPs were characterized by Fourier-transform infrared spectroscopy (FTIR), Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX), Ultraviolet–visible spectrophotometry (UV–Vis) and X-ray powder diffraction (XRD). FTIR spectrum confirmed the involvement of alkynes, alkanes, flavonoids and secondary alcohols in the capping and reduction of TiO2 NPs while XRD characterized the crystallographic nature of the TiO2 with a crystallite size 13.36, it also confirmed the presence of anatase and rutile forms of TiO2. Size of TiO2 NPs was in the range of 3–12 nm and EDX showed a strong TiO2 signal. Anti bacterial study revealed that both the bacterial strains were susceptible to the synthesized nanoparticles as dose dependent manner. The photocatalytic activity was observed to be higher at lower concentration of TiO2 NPs’ and with minimum exposure to UV. The TiO2 also showed anti-inflmmatory property as the protein degradation inhibition capacity was observed to be high. The present study implies that the presently proposed TiO2 nanoparticles can be used as an effective anti bacterial agent against food spoilage pathogens with photocatalytic activity.
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spelling doaj-art-e6c0b63abfef41d986e4253e94e822ca2025-08-20T03:10:21ZengElsevierThe Microbe2950-19462025-06-01710033110.1016/j.microb.2025.100331Anti bacterial efficacy of photo catalytic active titanium di oxide (TiO2) nanoparticles synthesized via green science principles against food spoilage pathogenic bacteriaL.F.A. Anand Raj0A. Annushrie1S. Karthick Raja Namasivayam2Department of Biotechnology, St. Joseph’s College of Engineering, OMR, Chennai 600119, India; Corresponding authors.Department of Biotechnology, St. Joseph’s College of Engineering, OMR, Chennai 600119, IndiaCentre for Applied Research, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu 602105, India; Corresponding authors.Green synthesis of nanoparticles is becoming one of the robust techniques, which may be suitable alternatives for chemical and physical methods. In the present research work, anti bacterial activity of green synthesized titanium di oxide (TiO2) nanoaparticles was screened against bacterial strains against food spoilage pathogenic bacterial strains Escherichia coli and Bacillus subtilis isolated from spoiled canned foods was studied followed by investigating anti oxidant and photocatalytic activity. Nanoparticles were fabricated from Talinum fruticosum leaf extract as the reducing agent. The green synthesized TiO2 NPs were characterized by Fourier-transform infrared spectroscopy (FTIR), Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX), Ultraviolet–visible spectrophotometry (UV–Vis) and X-ray powder diffraction (XRD). FTIR spectrum confirmed the involvement of alkynes, alkanes, flavonoids and secondary alcohols in the capping and reduction of TiO2 NPs while XRD characterized the crystallographic nature of the TiO2 with a crystallite size 13.36, it also confirmed the presence of anatase and rutile forms of TiO2. Size of TiO2 NPs was in the range of 3–12 nm and EDX showed a strong TiO2 signal. Anti bacterial study revealed that both the bacterial strains were susceptible to the synthesized nanoparticles as dose dependent manner. The photocatalytic activity was observed to be higher at lower concentration of TiO2 NPs’ and with minimum exposure to UV. The TiO2 also showed anti-inflmmatory property as the protein degradation inhibition capacity was observed to be high. The present study implies that the presently proposed TiO2 nanoparticles can be used as an effective anti bacterial agent against food spoilage pathogens with photocatalytic activity.http://www.sciencedirect.com/science/article/pii/S2950194625000998Titanium dioxide nanoprticlesTalinum fruticosumAnti bacterialFood spoilagePhotocatalyticAnti-inflammatory
spellingShingle L.F.A. Anand Raj
A. Annushrie
S. Karthick Raja Namasivayam
Anti bacterial efficacy of photo catalytic active titanium di oxide (TiO2) nanoparticles synthesized via green science principles against food spoilage pathogenic bacteria
The Microbe
Titanium dioxide nanoprticles
Talinum fruticosum
Anti bacterial
Food spoilage
Photocatalytic
Anti-inflammatory
title Anti bacterial efficacy of photo catalytic active titanium di oxide (TiO2) nanoparticles synthesized via green science principles against food spoilage pathogenic bacteria
title_full Anti bacterial efficacy of photo catalytic active titanium di oxide (TiO2) nanoparticles synthesized via green science principles against food spoilage pathogenic bacteria
title_fullStr Anti bacterial efficacy of photo catalytic active titanium di oxide (TiO2) nanoparticles synthesized via green science principles against food spoilage pathogenic bacteria
title_full_unstemmed Anti bacterial efficacy of photo catalytic active titanium di oxide (TiO2) nanoparticles synthesized via green science principles against food spoilage pathogenic bacteria
title_short Anti bacterial efficacy of photo catalytic active titanium di oxide (TiO2) nanoparticles synthesized via green science principles against food spoilage pathogenic bacteria
title_sort anti bacterial efficacy of photo catalytic active titanium di oxide tio2 nanoparticles synthesized via green science principles against food spoilage pathogenic bacteria
topic Titanium dioxide nanoprticles
Talinum fruticosum
Anti bacterial
Food spoilage
Photocatalytic
Anti-inflammatory
url http://www.sciencedirect.com/science/article/pii/S2950194625000998
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AT skarthickrajanamasivayam antibacterialefficacyofphotocatalyticactivetitaniumdioxidetio2nanoparticlessynthesizedviagreenscienceprinciplesagainstfoodspoilagepathogenicbacteria