MicroRNA‐9 exerts antitumor effects on hepatocellular carcinoma progression by targeting HMGA2
Accumulating evidence has demonstrated that the aberrant expression of microRNAs (miRs or miRNAs) may contribute to the initiation and progression of various types of human cancer and may also constitute biomarkers for cancer diagnosis and therapy. However, the specific function of miR‐9 in hepatoce...
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| Format: | Article |
| Language: | English |
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Wiley
2019-10-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.12716 |
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| author | Xiangang Xu Haibo Zou Lanyun Luo Xiankui Wang Guan Wang |
| author_facet | Xiangang Xu Haibo Zou Lanyun Luo Xiankui Wang Guan Wang |
| author_sort | Xiangang Xu |
| collection | DOAJ |
| description | Accumulating evidence has demonstrated that the aberrant expression of microRNAs (miRs or miRNAs) may contribute to the initiation and progression of various types of human cancer and may also constitute biomarkers for cancer diagnosis and therapy. However, the specific function of miR‐9 in hepatocellular carcinoma (HCC) remains unclear, and the mechanisms that underlie HCC are incompletely understood. Here, we report that miR‐9 expression was significantly decreased in clinical tumor tissue samples, as well as in a cohort of HCC cell lines. In addition, it was demonstrated that overexpression of miR‐9 suppressed the proliferative and migratory capacity of HCC cells and impaired cell cycle progression. Furthermore, high mobility group AT‐hook 2 (HMGA2) was verified as a downstream target gene of miR‐9 using a luciferase reporter assay. Quantitative RT‐PCR and western blotting implicated HMGA2 in the miR‐9‐mediated reduction of HCC cell growth. In vivo, transfection with miR‐9 mimics down‐regulated the expression of HMGA2, thus leading to a dramatic reduction in tumor growth in a mouse xenograft model. These results suggest that miR‐9 may exert critical antitumor effects on HCC by directly targeting HMGA2, and the miR9/HMGA2 signaling pathway may be of use for the diagnosis and prognosis of patients with HCC. |
| format | Article |
| id | doaj-art-e6bb461ef9724db2bb42f782aee0f237 |
| institution | OA Journals |
| issn | 2211-5463 |
| language | English |
| publishDate | 2019-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj-art-e6bb461ef9724db2bb42f782aee0f2372025-08-20T02:37:29ZengWileyFEBS Open Bio2211-54632019-10-019101784179710.1002/2211-5463.12716MicroRNA‐9 exerts antitumor effects on hepatocellular carcinoma progression by targeting HMGA2Xiangang Xu0Haibo Zou1Lanyun Luo2Xiankui Wang3Guan Wang4Department of Hepatobiliary Surgery Guizhou Provincial People's Hospital Guiyang ChinaDepartment of Hepatobiliary Surgery Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital Chengdu ChinaDepartment of Hepatobiliary Surgery Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital Chengdu ChinaDepartment of Hepatobiliary Surgery Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital Chengdu ChinaDepartment of Hepatobiliary Surgery Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital Chengdu ChinaAccumulating evidence has demonstrated that the aberrant expression of microRNAs (miRs or miRNAs) may contribute to the initiation and progression of various types of human cancer and may also constitute biomarkers for cancer diagnosis and therapy. However, the specific function of miR‐9 in hepatocellular carcinoma (HCC) remains unclear, and the mechanisms that underlie HCC are incompletely understood. Here, we report that miR‐9 expression was significantly decreased in clinical tumor tissue samples, as well as in a cohort of HCC cell lines. In addition, it was demonstrated that overexpression of miR‐9 suppressed the proliferative and migratory capacity of HCC cells and impaired cell cycle progression. Furthermore, high mobility group AT‐hook 2 (HMGA2) was verified as a downstream target gene of miR‐9 using a luciferase reporter assay. Quantitative RT‐PCR and western blotting implicated HMGA2 in the miR‐9‐mediated reduction of HCC cell growth. In vivo, transfection with miR‐9 mimics down‐regulated the expression of HMGA2, thus leading to a dramatic reduction in tumor growth in a mouse xenograft model. These results suggest that miR‐9 may exert critical antitumor effects on HCC by directly targeting HMGA2, and the miR9/HMGA2 signaling pathway may be of use for the diagnosis and prognosis of patients with HCC.https://doi.org/10.1002/2211-5463.12716antitumorhepatocellular carcinomahigh mobility group AT‐hook 2miR‐9miRNA |
| spellingShingle | Xiangang Xu Haibo Zou Lanyun Luo Xiankui Wang Guan Wang MicroRNA‐9 exerts antitumor effects on hepatocellular carcinoma progression by targeting HMGA2 FEBS Open Bio antitumor hepatocellular carcinoma high mobility group AT‐hook 2 miR‐9 miRNA |
| title | MicroRNA‐9 exerts antitumor effects on hepatocellular carcinoma progression by targeting HMGA2 |
| title_full | MicroRNA‐9 exerts antitumor effects on hepatocellular carcinoma progression by targeting HMGA2 |
| title_fullStr | MicroRNA‐9 exerts antitumor effects on hepatocellular carcinoma progression by targeting HMGA2 |
| title_full_unstemmed | MicroRNA‐9 exerts antitumor effects on hepatocellular carcinoma progression by targeting HMGA2 |
| title_short | MicroRNA‐9 exerts antitumor effects on hepatocellular carcinoma progression by targeting HMGA2 |
| title_sort | microrna 9 exerts antitumor effects on hepatocellular carcinoma progression by targeting hmga2 |
| topic | antitumor hepatocellular carcinoma high mobility group AT‐hook 2 miR‐9 miRNA |
| url | https://doi.org/10.1002/2211-5463.12716 |
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