The CIN-TCP transcription factors regulate endocycle progression and pavement cell size by promoting cell wall pectin degradation
Abstract In plants, endoreplication, the process where nuclear DNA replicates in the absence of mitosis, and remodeling of the primary cell walls are both coupled with cell expansion. However, the mechanisms by which these two processes coordinate to determine cell size remain largely elusive. Here,...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-05-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-59336-7 |
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| author | Feng Shen He Zhang Miaomiao Wan Yanzhi Yang Zheng Kuang Liang Xiao Daqing Zuo Zhan Li Genji Qin Lei Li |
| author_facet | Feng Shen He Zhang Miaomiao Wan Yanzhi Yang Zheng Kuang Liang Xiao Daqing Zuo Zhan Li Genji Qin Lei Li |
| author_sort | Feng Shen |
| collection | DOAJ |
| description | Abstract In plants, endoreplication, the process where nuclear DNA replicates in the absence of mitosis, and remodeling of the primary cell walls are both coupled with cell expansion. However, the mechanisms by which these two processes coordinate to determine cell size remain largely elusive. Here, employing the tcpΔ7 septuple mutant disabling seven of the eight CIN-TCP transcription factors in Arabidopsis, we find that hindered endoreplication progression in tcpΔ7 whereby ploidy increases from 8 C to beyond is correlated with an increase in cell wall pectin. CIN-TCPs transcriptionally activate POLYGALACTURONASE LIKE 1 (PGL1), which encodes a polygalacturonase downregulating both abundance and molecular mass of pectin polymers. Genetic analysis of PGL1 in both the wild type and tcpΔ7 backgrounds confirm that pectin reduction promotes endocycle progression and cell enlargement. Collectively, these findings reveal a critical role of pectin in regulating endoreplication, providing insights in the understanding of cell growth and organ development in plants. |
| format | Article |
| id | doaj-art-e6b76c64fb8f45958a1bc2f84065bfe5 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e6b76c64fb8f45958a1bc2f84065bfe52025-08-20T03:52:20ZengNature PortfolioNature Communications2041-17232025-05-0116111610.1038/s41467-025-59336-7The CIN-TCP transcription factors regulate endocycle progression and pavement cell size by promoting cell wall pectin degradationFeng Shen0He Zhang1Miaomiao Wan2Yanzhi Yang3Zheng Kuang4Liang Xiao5Daqing Zuo6Zhan Li7Genji Qin8Lei Li9Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangPeking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangPeking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangPeking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangSchool of Advanced Agricultural Sciences and School of Life Sciences, Peking UniversityPeking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangPeking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangSchool of Advanced Agricultural Sciences and School of Life Sciences, Peking UniversitySchool of Advanced Agricultural Sciences and School of Life Sciences, Peking UniversityPeking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangAbstract In plants, endoreplication, the process where nuclear DNA replicates in the absence of mitosis, and remodeling of the primary cell walls are both coupled with cell expansion. However, the mechanisms by which these two processes coordinate to determine cell size remain largely elusive. Here, employing the tcpΔ7 septuple mutant disabling seven of the eight CIN-TCP transcription factors in Arabidopsis, we find that hindered endoreplication progression in tcpΔ7 whereby ploidy increases from 8 C to beyond is correlated with an increase in cell wall pectin. CIN-TCPs transcriptionally activate POLYGALACTURONASE LIKE 1 (PGL1), which encodes a polygalacturonase downregulating both abundance and molecular mass of pectin polymers. Genetic analysis of PGL1 in both the wild type and tcpΔ7 backgrounds confirm that pectin reduction promotes endocycle progression and cell enlargement. Collectively, these findings reveal a critical role of pectin in regulating endoreplication, providing insights in the understanding of cell growth and organ development in plants.https://doi.org/10.1038/s41467-025-59336-7 |
| spellingShingle | Feng Shen He Zhang Miaomiao Wan Yanzhi Yang Zheng Kuang Liang Xiao Daqing Zuo Zhan Li Genji Qin Lei Li The CIN-TCP transcription factors regulate endocycle progression and pavement cell size by promoting cell wall pectin degradation Nature Communications |
| title | The CIN-TCP transcription factors regulate endocycle progression and pavement cell size by promoting cell wall pectin degradation |
| title_full | The CIN-TCP transcription factors regulate endocycle progression and pavement cell size by promoting cell wall pectin degradation |
| title_fullStr | The CIN-TCP transcription factors regulate endocycle progression and pavement cell size by promoting cell wall pectin degradation |
| title_full_unstemmed | The CIN-TCP transcription factors regulate endocycle progression and pavement cell size by promoting cell wall pectin degradation |
| title_short | The CIN-TCP transcription factors regulate endocycle progression and pavement cell size by promoting cell wall pectin degradation |
| title_sort | cin tcp transcription factors regulate endocycle progression and pavement cell size by promoting cell wall pectin degradation |
| url | https://doi.org/10.1038/s41467-025-59336-7 |
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