Impact of respiratory syncytial virus on Streptococcus pneumoniae resistance to β-lactam antibiotics: an investigative study in children
Abstract Background This study characterizes the molecular interplay between respiratory syncytial virus (RSV) glycoproteins (G/F) and Streptococcus pneumoniae (S.pn) penicillin-binding proteins (PBPs), while evaluating RSV’s potential role in modulating S.pn β-lactam resistance. Methods Protein doc...
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2025-06-01
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| Online Access: | https://doi.org/10.1186/s12866-025-04066-5 |
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| author | Lu Li Yushun Wan Yuncheng Wang Ye Tao Xiao Long Enmei Liu Yu Deng |
| author_facet | Lu Li Yushun Wan Yuncheng Wang Ye Tao Xiao Long Enmei Liu Yu Deng |
| author_sort | Lu Li |
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| description | Abstract Background This study characterizes the molecular interplay between respiratory syncytial virus (RSV) glycoproteins (G/F) and Streptococcus pneumoniae (S.pn) penicillin-binding proteins (PBPs), while evaluating RSV’s potential role in modulating S.pn β-lactam resistance. Methods Protein docking and pull-down assays assessed G/F-PBP interactions. In vitro RSV-S.pn co-culture experiments evaluated β-lactam susceptibility (MIC determination). We retrospectively analyzed 2012–2021 antimicrobial resistance data from 1-59-month-old community-acquired pneumonia patients at Chongqing Medical University Children’s Hospital with confirmed S.pn and/or RSV nasopharyngeal carriage. Results Computational modeling revealed low G/F-PBP binding affinity (iPTM < 0.6), corroborated by absent PBP1a interaction in pull-down assays. RSV exposure did not alter S.pn β-lactam MICs (penicillin/amoxicillin ≤ 2 µg/mL; cefepime/cefotaxime ≤ 1 µg/mL; meropenem ≤ 0.25 µg/mL). Retrospective data showed elevated penicillin resistance in RSV + S.pn co-detections vs. S.pn alone during 2012 (2.8% vs. 40.9%), 2017 (2.8% vs. 30.4%), and 2018 (6.2% vs. 38.6%) (all p < 0.001). No RSV-associated resistance increases occurred for amoxicillin, cephalosporins, or meropenem. Conclusions RSV demonstrates negligible impact on S.pn β-lactam resistance mechanisms, elevated resistance rates to amoxicillin and cephalosporins necessitate enhanced antimicrobial stewardship through diagnostic-guided prescribing and resistance surveillance to optimize β-lactam efficacy in pediatric care. |
| format | Article |
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| spelling | doaj-art-e6ad6fb7bff046edb4ec438dd41b85022025-08-20T02:06:20ZengBMCBMC Microbiology1471-21802025-06-012511910.1186/s12866-025-04066-5Impact of respiratory syncytial virus on Streptococcus pneumoniae resistance to β-lactam antibiotics: an investigative study in childrenLu Li0Yushun Wan1Yuncheng Wang2Ye Tao3Xiao Long4Enmei Liu5Yu Deng6Department of Respiratory Medicine, National Clinical Research for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Child Rare Diseases in Infection and Immunity, Key Laboratory of Children’s Important Organ Development and Diseases of Chongqing Municipal Health Commission, Children’s Hospital of Chongqing Medical UniversityDepartment of Respiratory Medicine, National Clinical Research for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Child Rare Diseases in Infection and Immunity, Key Laboratory of Children’s Important Organ Development and Diseases of Chongqing Municipal Health Commission, Children’s Hospital of Chongqing Medical UniversityCollege of Basic Medicine of Chongqing Medical UniversityDepartment of Laboratory of Children’s Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of PediatricsDepartment of Respiratory Medicine, National Clinical Research for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Child Rare Diseases in Infection and Immunity, Key Laboratory of Children’s Important Organ Development and Diseases of Chongqing Municipal Health Commission, Children’s Hospital of Chongqing Medical UniversityDepartment of Respiratory Medicine, National Clinical Research for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Child Rare Diseases in Infection and Immunity, Key Laboratory of Children’s Important Organ Development and Diseases of Chongqing Municipal Health Commission, Children’s Hospital of Chongqing Medical UniversityDepartment of Respiratory Medicine, National Clinical Research for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Child Rare Diseases in Infection and Immunity, Key Laboratory of Children’s Important Organ Development and Diseases of Chongqing Municipal Health Commission, Children’s Hospital of Chongqing Medical UniversityAbstract Background This study characterizes the molecular interplay between respiratory syncytial virus (RSV) glycoproteins (G/F) and Streptococcus pneumoniae (S.pn) penicillin-binding proteins (PBPs), while evaluating RSV’s potential role in modulating S.pn β-lactam resistance. Methods Protein docking and pull-down assays assessed G/F-PBP interactions. In vitro RSV-S.pn co-culture experiments evaluated β-lactam susceptibility (MIC determination). We retrospectively analyzed 2012–2021 antimicrobial resistance data from 1-59-month-old community-acquired pneumonia patients at Chongqing Medical University Children’s Hospital with confirmed S.pn and/or RSV nasopharyngeal carriage. Results Computational modeling revealed low G/F-PBP binding affinity (iPTM < 0.6), corroborated by absent PBP1a interaction in pull-down assays. RSV exposure did not alter S.pn β-lactam MICs (penicillin/amoxicillin ≤ 2 µg/mL; cefepime/cefotaxime ≤ 1 µg/mL; meropenem ≤ 0.25 µg/mL). Retrospective data showed elevated penicillin resistance in RSV + S.pn co-detections vs. S.pn alone during 2012 (2.8% vs. 40.9%), 2017 (2.8% vs. 30.4%), and 2018 (6.2% vs. 38.6%) (all p < 0.001). No RSV-associated resistance increases occurred for amoxicillin, cephalosporins, or meropenem. Conclusions RSV demonstrates negligible impact on S.pn β-lactam resistance mechanisms, elevated resistance rates to amoxicillin and cephalosporins necessitate enhanced antimicrobial stewardship through diagnostic-guided prescribing and resistance surveillance to optimize β-lactam efficacy in pediatric care.https://doi.org/10.1186/s12866-025-04066-5Respiratory syncytial virusStreptococcus pneumoniaeAntibiotic resistanceGlycoproteinPenicillin-binding protein |
| spellingShingle | Lu Li Yushun Wan Yuncheng Wang Ye Tao Xiao Long Enmei Liu Yu Deng Impact of respiratory syncytial virus on Streptococcus pneumoniae resistance to β-lactam antibiotics: an investigative study in children BMC Microbiology Respiratory syncytial virus Streptococcus pneumoniae Antibiotic resistance Glycoprotein Penicillin-binding protein |
| title | Impact of respiratory syncytial virus on Streptococcus pneumoniae resistance to β-lactam antibiotics: an investigative study in children |
| title_full | Impact of respiratory syncytial virus on Streptococcus pneumoniae resistance to β-lactam antibiotics: an investigative study in children |
| title_fullStr | Impact of respiratory syncytial virus on Streptococcus pneumoniae resistance to β-lactam antibiotics: an investigative study in children |
| title_full_unstemmed | Impact of respiratory syncytial virus on Streptococcus pneumoniae resistance to β-lactam antibiotics: an investigative study in children |
| title_short | Impact of respiratory syncytial virus on Streptococcus pneumoniae resistance to β-lactam antibiotics: an investigative study in children |
| title_sort | impact of respiratory syncytial virus on streptococcus pneumoniae resistance to β lactam antibiotics an investigative study in children |
| topic | Respiratory syncytial virus Streptococcus pneumoniae Antibiotic resistance Glycoprotein Penicillin-binding protein |
| url | https://doi.org/10.1186/s12866-025-04066-5 |
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