Selection of <i>Bactrocera tau</i> (Walker) Reference Genes for Quantitative Real-Time PCR
The selection of appropriate reference genes is critical for standardizing quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) data, thereby ensuring accurate and reliable results of gene expression analysis. In this study, we identified 10 candidate reference genes (enc...
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MDPI AG
2025-04-01
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| author | Yutong Zhai Yonghao Yu Pengfei Xu Xianru Zeng Xiuzhen Long Dewei Wei Zhan He Xuyuan Gao |
| author_facet | Yutong Zhai Yonghao Yu Pengfei Xu Xianru Zeng Xiuzhen Long Dewei Wei Zhan He Xuyuan Gao |
| author_sort | Yutong Zhai |
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| description | The selection of appropriate reference genes is critical for standardizing quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) data, thereby ensuring accurate and reliable results of gene expression analysis. In this study, we identified 10 candidate reference genes (encoding <i>α-tubulin</i>, <i>G6PDH</i>, <i>Rab1</i>, <i>RT</i>, <i>RPS13</i>, <i>β-tubulin</i>, <i>DPH1</i>, <i>HSP90</i>, <i>GAPDH</i>, and <i>CP</i>) and evaluated their suitability for use as reference genes in the pest insect, <i>Bactrocera tau</i>. Analysis was conducted using three software-based methods—Delta CT, NormFinder, and BestKeeper—alongside the online tool RefFinder. Expression levels of these genes were analyzed across various <i>B. tau</i> developmental stages and body parts. The overall ranking of reference gene stability scores was as follows: <i>α-tubulin</i> > <i>G6PDH</i> > <i>CP</i> > <i>β-tubulin</i> > <i>RT</i> > <i>HSP90</i> > <i>GAPDH</i> > <i>DPH1</i> > <i>RPS13</i> > <i>Rab1</i>. Ultimately, <i>α-tubulin</i> and <i>G6PDH</i> were identified as the most stable reference genes for <i>B. tau</i>. |
| format | Article |
| id | doaj-art-e6a9cdfc0995465e8ff0a7797cba4237 |
| institution | Kabale University |
| issn | 2075-4450 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj-art-e6a9cdfc0995465e8ff0a7797cba42372025-08-20T03:47:57ZengMDPI AGInsects2075-44502025-04-0116544510.3390/insects16050445Selection of <i>Bactrocera tau</i> (Walker) Reference Genes for Quantitative Real-Time PCRYutong Zhai0Yonghao Yu1Pengfei Xu2Xianru Zeng3Xiuzhen Long4Dewei Wei5Zhan He6Xuyuan Gao7Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaPlant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaPlant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaPlant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaPlant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaPlant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaPlant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaPlant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaThe selection of appropriate reference genes is critical for standardizing quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) data, thereby ensuring accurate and reliable results of gene expression analysis. In this study, we identified 10 candidate reference genes (encoding <i>α-tubulin</i>, <i>G6PDH</i>, <i>Rab1</i>, <i>RT</i>, <i>RPS13</i>, <i>β-tubulin</i>, <i>DPH1</i>, <i>HSP90</i>, <i>GAPDH</i>, and <i>CP</i>) and evaluated their suitability for use as reference genes in the pest insect, <i>Bactrocera tau</i>. Analysis was conducted using three software-based methods—Delta CT, NormFinder, and BestKeeper—alongside the online tool RefFinder. Expression levels of these genes were analyzed across various <i>B. tau</i> developmental stages and body parts. The overall ranking of reference gene stability scores was as follows: <i>α-tubulin</i> > <i>G6PDH</i> > <i>CP</i> > <i>β-tubulin</i> > <i>RT</i> > <i>HSP90</i> > <i>GAPDH</i> > <i>DPH1</i> > <i>RPS13</i> > <i>Rab1</i>. Ultimately, <i>α-tubulin</i> and <i>G6PDH</i> were identified as the most stable reference genes for <i>B. tau</i>.https://www.mdpi.com/2075-4450/16/5/445reference genesRT-qPCR<i>Bactrocera tau</i>different stagesdifferent body parts |
| spellingShingle | Yutong Zhai Yonghao Yu Pengfei Xu Xianru Zeng Xiuzhen Long Dewei Wei Zhan He Xuyuan Gao Selection of <i>Bactrocera tau</i> (Walker) Reference Genes for Quantitative Real-Time PCR Insects reference genes RT-qPCR <i>Bactrocera tau</i> different stages different body parts |
| title | Selection of <i>Bactrocera tau</i> (Walker) Reference Genes for Quantitative Real-Time PCR |
| title_full | Selection of <i>Bactrocera tau</i> (Walker) Reference Genes for Quantitative Real-Time PCR |
| title_fullStr | Selection of <i>Bactrocera tau</i> (Walker) Reference Genes for Quantitative Real-Time PCR |
| title_full_unstemmed | Selection of <i>Bactrocera tau</i> (Walker) Reference Genes for Quantitative Real-Time PCR |
| title_short | Selection of <i>Bactrocera tau</i> (Walker) Reference Genes for Quantitative Real-Time PCR |
| title_sort | selection of i bactrocera tau i walker reference genes for quantitative real time pcr |
| topic | reference genes RT-qPCR <i>Bactrocera tau</i> different stages different body parts |
| url | https://www.mdpi.com/2075-4450/16/5/445 |
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