Discriminative spatial localisation in confocal reflectance and carbon dots-based fluorescence imaging using mixed-mode endoscopic scanner
Carbon dots (CDs)-based fluorescence imaging with confocal reflectance effectively localises labelled structures with clear sample visualization. Yet, integrating these modalities into single-fibre cantilever-based endoscopic scanners poses challenges. Thus, we present an endoscopic scanner with a m...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | International Journal of Optomechatronics |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/15599612.2024.2347292 |
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| author | Yang Sing Leong Mohd Hadri Hafiz Mokhtar Nur Afifah Ahmad Nazri Mohd Hafiz Abu Bakar Mohd Saiful Dzulkefly Zan Norhana Arsad Peer Mohamed Abdul Ahmad Ashrif A. Bakar |
| author_facet | Yang Sing Leong Mohd Hadri Hafiz Mokhtar Nur Afifah Ahmad Nazri Mohd Hafiz Abu Bakar Mohd Saiful Dzulkefly Zan Norhana Arsad Peer Mohamed Abdul Ahmad Ashrif A. Bakar |
| author_sort | Yang Sing Leong |
| collection | DOAJ |
| description | Carbon dots (CDs)-based fluorescence imaging with confocal reflectance effectively localises labelled structures with clear sample visualization. Yet, integrating these modalities into single-fibre cantilever-based endoscopic scanners poses challenges. Thus, we present an endoscopic scanner with a mixed-mode fibre cantilever for dual modalities imaging. The cantilever features two-attached fibre construction for multiple illumination and detection channels without additional optical components. The measured lateral and axial resolutions are 5.81 and 6.52 µm, respectively with field-of-view of 408 × 488 µm. The fluorescence detection capability was first verified using fluorescent microspheres and CD-embedded polymer film, followed by confocal reflectance and CDs-based fluorescence imaging on labelled fibrous network and plant samples. The results demonstrate the complementary strengths of confocal reflectance, which extracts surface profiles, and CDs-based fluorescence, which captures fluorescence signals, leading to precise localization of fluorescence signals within the sample. Switching modality schemes at the scanner’s back-end enables implementation of different modalities. |
| format | Article |
| id | doaj-art-e6a8bdcc57234c31bc2aa09004ffa693 |
| institution | OA Journals |
| issn | 1559-9612 1559-9620 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Optomechatronics |
| spelling | doaj-art-e6a8bdcc57234c31bc2aa09004ffa6932025-08-20T01:58:49ZengTaylor & Francis GroupInternational Journal of Optomechatronics1559-96121559-96202024-12-0118110.1080/15599612.2024.2347292Discriminative spatial localisation in confocal reflectance and carbon dots-based fluorescence imaging using mixed-mode endoscopic scannerYang Sing Leong0Mohd Hadri Hafiz Mokhtar1Nur Afifah Ahmad Nazri2Mohd Hafiz Abu Bakar3Mohd Saiful Dzulkefly Zan4Norhana Arsad5Peer Mohamed Abdul6Ahmad Ashrif A. Bakar7Department of Electrical, Electronic and Systems Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, Selangor, MalaysiaDepartment of Electrical, Electronic and Systems Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, Selangor, MalaysiaDepartment of Electrical, Electronic and Systems Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, Selangor, MalaysiaInstitute of Power Engineering, Universiti Tenaga Nasional, Kajang, Selangor, MalaysiaDepartment of Electrical, Electronic and Systems Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, Selangor, MalaysiaDepartment of Electrical, Electronic and Systems Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, Selangor, MalaysiaDepartment of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, MalaysiaDepartment of Electrical, Electronic and Systems Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, Selangor, MalaysiaCarbon dots (CDs)-based fluorescence imaging with confocal reflectance effectively localises labelled structures with clear sample visualization. Yet, integrating these modalities into single-fibre cantilever-based endoscopic scanners poses challenges. Thus, we present an endoscopic scanner with a mixed-mode fibre cantilever for dual modalities imaging. The cantilever features two-attached fibre construction for multiple illumination and detection channels without additional optical components. The measured lateral and axial resolutions are 5.81 and 6.52 µm, respectively with field-of-view of 408 × 488 µm. The fluorescence detection capability was first verified using fluorescent microspheres and CD-embedded polymer film, followed by confocal reflectance and CDs-based fluorescence imaging on labelled fibrous network and plant samples. The results demonstrate the complementary strengths of confocal reflectance, which extracts surface profiles, and CDs-based fluorescence, which captures fluorescence signals, leading to precise localization of fluorescence signals within the sample. Switching modality schemes at the scanner’s back-end enables implementation of different modalities.https://www.tandfonline.com/doi/10.1080/15599612.2024.2347292Lissajous scanningconfocal reflectancefluorescence imagingendoscopic scannercarbon dots |
| spellingShingle | Yang Sing Leong Mohd Hadri Hafiz Mokhtar Nur Afifah Ahmad Nazri Mohd Hafiz Abu Bakar Mohd Saiful Dzulkefly Zan Norhana Arsad Peer Mohamed Abdul Ahmad Ashrif A. Bakar Discriminative spatial localisation in confocal reflectance and carbon dots-based fluorescence imaging using mixed-mode endoscopic scanner International Journal of Optomechatronics Lissajous scanning confocal reflectance fluorescence imaging endoscopic scanner carbon dots |
| title | Discriminative spatial localisation in confocal reflectance and carbon dots-based fluorescence imaging using mixed-mode endoscopic scanner |
| title_full | Discriminative spatial localisation in confocal reflectance and carbon dots-based fluorescence imaging using mixed-mode endoscopic scanner |
| title_fullStr | Discriminative spatial localisation in confocal reflectance and carbon dots-based fluorescence imaging using mixed-mode endoscopic scanner |
| title_full_unstemmed | Discriminative spatial localisation in confocal reflectance and carbon dots-based fluorescence imaging using mixed-mode endoscopic scanner |
| title_short | Discriminative spatial localisation in confocal reflectance and carbon dots-based fluorescence imaging using mixed-mode endoscopic scanner |
| title_sort | discriminative spatial localisation in confocal reflectance and carbon dots based fluorescence imaging using mixed mode endoscopic scanner |
| topic | Lissajous scanning confocal reflectance fluorescence imaging endoscopic scanner carbon dots |
| url | https://www.tandfonline.com/doi/10.1080/15599612.2024.2347292 |
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